背景:有研究表明,超声增强抗生素杀菌能力的效力表现为强度依赖性,即超声强度越高其效力越大。目的:观察不同强度超声对金黄色葡萄球菌及其生物膜的破坏作用。方法:采用引导片培养法体外培养金黄色葡萄球菌生物膜,分为3组干预,对照组不...背景:有研究表明,超声增强抗生素杀菌能力的效力表现为强度依赖性,即超声强度越高其效力越大。目的:观察不同强度超声对金黄色葡萄球菌及其生物膜的破坏作用。方法:采用引导片培养法体外培养金黄色葡萄球菌生物膜,分为3组干预,对照组不作任何处理;低强度超声组采用强度为500 m W/cm、频率为200 k Hz的脉冲超声波干预10 min;高强度超声组采用强度为40 W、频率1 MHz的连续超声波干预10 min。采用超声振荡-活菌计数法计数细菌菌落;采用碘化丙啶(PI)和FITC-Con A分别标记细菌DNA和多糖,激光扫描共聚焦显微镜检测生物膜成型情况。结果与结论:高强度超声组细菌菌落低于对照组、低强度超声组(P<0.05),后两组间细菌菌落比较差异无显著性意义。低强度超声组红色荧光和绿色荧光的数量及密集程度与对照组无明显区别,高强度超声组的红色荧光和绿色荧光相对于前两组有明显减少。表明低强度超声不能杀灭细菌且对细菌生物膜的破坏作用微小,高强度超声能有效杀灭细菌,并且对细菌生物膜具有显著破坏作用。展开更多
The anti-virus and anti-bacteria active components were extracted from some Chinese medicine,such as the honeysuckle,forsythia and the licorice.Using a w/o/w emulsion method,the active components were fabricated to un...The anti-virus and anti-bacteria active components were extracted from some Chinese medicine,such as the honeysuckle,forsythia and the licorice.Using a w/o/w emulsion method,the active components were fabricated to uniform particulate microcapsule with sustained-release properties.The polypropylene punched felt was finished with the finishing agent of microcapsule,nano ZnO and TiO 2 and polymer adhesive,and the composite air filter with anti-virus and anti-bacteria properties were formed,staphylococcus aureus,colibacillus and candida albicans were applied to antibacterial experiments.The results indicate that the anti-bacteria rate are all 100%,and the virus inactivation rate also reaches 100% to pandemic influenza A virus.展开更多
基金the National Natural Science Foundation of China,No.81260228,81460278the Natural Science Foundation of Yunnan Province,China,No.2013FZ276,2014FA048Yunnan Provincial High-level Personnel Training Fund,China,No.2012HB032,D-201222~~
文摘背景:有研究表明,超声增强抗生素杀菌能力的效力表现为强度依赖性,即超声强度越高其效力越大。目的:观察不同强度超声对金黄色葡萄球菌及其生物膜的破坏作用。方法:采用引导片培养法体外培养金黄色葡萄球菌生物膜,分为3组干预,对照组不作任何处理;低强度超声组采用强度为500 m W/cm、频率为200 k Hz的脉冲超声波干预10 min;高强度超声组采用强度为40 W、频率1 MHz的连续超声波干预10 min。采用超声振荡-活菌计数法计数细菌菌落;采用碘化丙啶(PI)和FITC-Con A分别标记细菌DNA和多糖,激光扫描共聚焦显微镜检测生物膜成型情况。结果与结论:高强度超声组细菌菌落低于对照组、低强度超声组(P<0.05),后两组间细菌菌落比较差异无显著性意义。低强度超声组红色荧光和绿色荧光的数量及密集程度与对照组无明显区别,高强度超声组的红色荧光和绿色荧光相对于前两组有明显减少。表明低强度超声不能杀灭细菌且对细菌生物膜的破坏作用微小,高强度超声能有效杀灭细菌,并且对细菌生物膜具有显著破坏作用。
基金supported by the National Science and Technology Small and Medium Sized Enterprises Technological Innovation Fund (Grant No.10C26213201127)Key Laboratory Foundation of Shannxi Province Education Department (Grant No.11JS044)the Shannxi Province Science and Technique Innovation Fund (Grant No. 2010K10-09)
文摘The anti-virus and anti-bacteria active components were extracted from some Chinese medicine,such as the honeysuckle,forsythia and the licorice.Using a w/o/w emulsion method,the active components were fabricated to uniform particulate microcapsule with sustained-release properties.The polypropylene punched felt was finished with the finishing agent of microcapsule,nano ZnO and TiO 2 and polymer adhesive,and the composite air filter with anti-virus and anti-bacteria properties were formed,staphylococcus aureus,colibacillus and candida albicans were applied to antibacterial experiments.The results indicate that the anti-bacteria rate are all 100%,and the virus inactivation rate also reaches 100% to pandemic influenza A virus.
