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体外拮抗幽门螺杆菌抑菌方法的研究 被引量:2
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作者 喻钢 田万红 +3 位作者 王斌 许文香 王军志 曾明 《药物分析杂志》 CAS CSCD 北大核心 2014年第10期1897-1901,共5页
目的:建立和优化针对幽门螺杆菌的体外抑菌方法,为筛选拮抗幽门螺杆菌的菌株和活性物质提供简单有效的操作方法。方法:将幽门螺杆菌NCTC 11637国际标准株作为指示菌,比较研究打孔扩散法、纸片法、点状覆盖法、共培养活菌计数法等常规抑... 目的:建立和优化针对幽门螺杆菌的体外抑菌方法,为筛选拮抗幽门螺杆菌的菌株和活性物质提供简单有效的操作方法。方法:将幽门螺杆菌NCTC 11637国际标准株作为指示菌,比较研究打孔扩散法、纸片法、点状覆盖法、共培养活菌计数法等常规抑菌方法,对各种方法在体外拮抗幽门螺杆菌实验的适用性、操作难度、判定时间等方面进行评价。结果:纸片法样品装载量少,点状覆盖法无法检测,活菌计数法操作烦琐,批处理量小,而优化后的打孔扩散法具有简单高效,判定时间短,观察结果直观等特点。结论:优化后的打孔扩散法符合幽门螺杆菌体外拮抗实验方法的要求,适用于多样品检测,为筛选拮抗幽门螺杆菌菌株和分离纯化活性物质过程中的各步骤间活性检测提供了极大的帮助。 展开更多
关键词 幽门螺杆 体外抑试验方法 枯草芽孢杆 抗菌活性物质筛选
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Phytochemical Screening and Antimicrobial Evaluation of the Aqueous Extracts of Ammoides verticillata, an Endemic Species
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作者 Oumessaad Toubal Abdelghani Djahoudi +1 位作者 Cherifa Henchiri Mohamed Bouazza 《Journal of Life Sciences》 2012年第3期243-247,共5页
Ammoides verticillata (Desf.) Briq. is an Algerian endemic species; the phytochemical screening (methods of Harborne, 1973) of the aerials parts wealth in polyphenol compounds: flavonoids, saponins, leucoanthocya... Ammoides verticillata (Desf.) Briq. is an Algerian endemic species; the phytochemical screening (methods of Harborne, 1973) of the aerials parts wealth in polyphenol compounds: flavonoids, saponins, leucoanthocyanes, terpens and steroids and tannins; there is no alkaloids. There is an important quantity of essential oils in the flowers; the interest of this study is that this species remains until then it is not very known. The results of Aromatogram method by incorporation of Mtiller-Hinton on solid medium, showed a significant antimicrobial activity (method of Duraffourd, 1987) of the infusion and the ethanolic extract; the infusion of stems and flowers is indeed much more active on Echerichia coli, Citrobacter, Enterobacter, Staph aureus and Staph epidermidis, such as flowers extracts demonstrate an important antimicrobial activity on Staph aureus, Staph epidermidis, Streptococcus, Pseudomonas and Acinetobacter recognized as antibiotic resistant. This could give opportunities for using this species in the treatment of diverse infections and as a disinfecting additive on nosocomial area. The valorization, preservation and sustainable use ofAmmoides verticillata require the protection of its habitats. 展开更多
关键词 Ammoides verticillata aqueous extracts phytochemical screening antimicrobial activity disinfection.
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In vitro Antimicrobial Activity and Phytochemical Screening of Local Plants
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作者 Shital Chandrashekhar Lolge Sunita Pandit Zanke +1 位作者 Dipfi Ramrao Patil Sureshchandra Popat Zambare 《Journal of Pharmacy and Pharmacology》 2016年第4期151-154,共4页
Antimicrobial activity of extracts of local plants was evaluated, with two strain i.e E. coil and S. aurri (gram positive and gram negative) microorganisms. The in vitro anti bacterial activity was performed by agar... Antimicrobial activity of extracts of local plants was evaluated, with two strain i.e E. coil and S. aurri (gram positive and gram negative) microorganisms. The in vitro anti bacterial activity was performed by agar disc diffusion method. The most active antibacterial plants were S. trifiliatus and A. mexicana. The significant antibacterial activity of active extracts was compared with the standered, antimicrobics, streptomycin (10 μg/disc). The results obtained in the present study suggest that S. trifoliatus and A. mexicana can be used in treating diseases caused by the test organism. 展开更多
关键词 ANTIMICROBIAL ANTIBACTERIAL plant extract S. trifoliatus A. mexicana.
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