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抗菌肽在细菌感染动物模型中的抗菌活性和毒性评价方法研究进展 被引量:4
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作者 黄海凤 缑三虎 +1 位作者 马银云 倪京满 《动物医学进展》 北大核心 2016年第3期103-108,共6页
抗菌肽作为新型的潜在抗菌药物,在耐药问题日益严峻的今天引起了广泛关注。在对抗菌肽体外研究不断深入的同时,抗菌肽体内研究也逐渐成为关注重点。论文主要从抗菌肽的体内抗菌活性研究和毒性评价两个方面进行了综述,在体内抗菌活性研... 抗菌肽作为新型的潜在抗菌药物,在耐药问题日益严峻的今天引起了广泛关注。在对抗菌肽体外研究不断深入的同时,抗菌肽体内研究也逐渐成为关注重点。论文主要从抗菌肽的体内抗菌活性研究和毒性评价两个方面进行了综述,在体内抗菌活性研究中介绍了模型设计及动物试验原则、接种途径和模型鉴定方法,并对常用于抗菌肽体内研究的几种典型细菌感染动物模型建立及活性试验方法进行了总结,在毒性评价中主要介绍了测定半数致死量(LD50)法,同时对建模及活性毒性评价研究中的不同方法进行了对比分析。 展开更多
关键词 动物模型 抗菌活性评价 毒性评价
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青楷槭抗菌药皂的制备
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作者 巩凤蓓 杨娜 +3 位作者 王松泽 张美慧 高航 崔恩姬 《山东化工》 CAS 2021年第19期45-46,49,共3页
本实验以抗菌活性为导向,筛选对青楷槭树皮活性最强的提取方法,发现70%乙醇冷浸法为最佳提取方法,且青楷槭树皮70%乙醇提取的乙酸乙酯层为活性部位。为了利用活性部位制备抗菌药皂,以抗菌活性为指标,优化抗菌药皂的制备工艺。结果表明,... 本实验以抗菌活性为导向,筛选对青楷槭树皮活性最强的提取方法,发现70%乙醇冷浸法为最佳提取方法,且青楷槭树皮70%乙醇提取的乙酸乙酯层为活性部位。为了利用活性部位制备抗菌药皂,以抗菌活性为指标,优化抗菌药皂的制备工艺。结果表明,最佳制备工艺是:乙酸乙酯萃取物用量为15 mg,甘油用量为300μL,温度为80℃。 展开更多
关键词 青楷槭 抗菌活性评价 提取方法 药皂制备
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纺织品抗菌整理 被引量:2
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作者 P.Senthilkumar 刘蒙稣 《国际纺织导报》 2017年第5期34-35,50,共3页
随着消费者健康生活方式意识的与日俱增,对大部分纺织品进行抗菌整理势在必行。纺织纤维固有的特性为细菌的滋长提供了空间,基布的结构和化学加工过程也会导致细菌的滋长,温暖与潮湿的环境加剧了此问题。细菌感染会引起病原体交叉感染,... 随着消费者健康生活方式意识的与日俱增,对大部分纺织品进行抗菌整理势在必行。纺织纤维固有的特性为细菌的滋长提供了空间,基布的结构和化学加工过程也会导致细菌的滋长,温暖与潮湿的环境加剧了此问题。细菌感染会引起病原体交叉感染,并使贴身衣物散发出异味。细菌侵害还会导致纺织基布的沾污和变质。鉴于此,为保护穿着者和纺织基布本身,需对纺织材料进行抗菌整理。 展开更多
关键词 纺织品 整理 机理 抗菌活性评价
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Preliminary Studies and Antimicrobial Evaluation of the Aerial Parts of Genista numidica ssp. numidica 被引量:1
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作者 Oumessaad Toubal Abdelghani Djahoudi Amel Bouzabata 《Journal of Life Sciences》 2011年第11期954-959,共6页
Genista numidica Spach ssp numidica (Spach) Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aeri... Genista numidica Spach ssp numidica (Spach) Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aerial parts of this species (stems, flowers, seeds and pods); there is a wealth in polyphenolic compounds: flavonoids (method of Charaux, Paris, 1964) and saponins (method of Braune et al, 1969), tannins, terpenes, sterols and fatty acids. The microscopic analysis doesn't allow a differential identification of the species. The results of aromatogram by the incorporation method of Muller-Hinton on solid medium, showed a significant antimicrobial activity (method Duraffourd,1987) of the extracts, infusion, and the active substances towards bacterial strains, essentially those recognized as antibiotic resistant (Staphylococcus, Acinetobacter, Pseudomonas, Enterobacter, Citrobacter); the results obtained indicate that the most efficient antibacterial activity was obtained with the pod infusion, the seed extract and the flavonoids. The raw extract has an important antifungal activity on Candida albicans. This could open opportunities for using this species in the treatment of urinary, respiratory, intestinals and cutaneous infections, and as a disinfecting additive on nosocomial area. 展开更多
关键词 Genista numidica ssp. numidica phytochemical screening fatty acids antimicrobial activity DISINFECTION
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Isolation and Antifungal Evaluation of Rumex cyprius Murb Extracts
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作者 Ahmad Ibrahim Husem Mohammed A. AI-Nuri +2 位作者 Nidal A. Zatar Waheed Jondi Mohammed Saleem Ali-Shtayeh 《Journal of Chemistry and Chemical Engineering》 2012年第6期547-550,共4页
Ethanolic extract of dried Rumex cyprius Murb (polygonaceae) was tested against four pathogenic fungi: three dermatophytes, Microsporum canis, Trichophyton mentagrophytes and T. rubrum, and the causative agent of c... Ethanolic extract of dried Rumex cyprius Murb (polygonaceae) was tested against four pathogenic fungi: three dermatophytes, Microsporum canis, Trichophyton mentagrophytes and T. rubrum, and the causative agent of chalkbrood disease of bees, Ascophaera apis. The extract showed considerable activity against all these fungi. One of the main constituents ofRumex cyprius was isolated and tested for its antimycotic activity. It showed a significant activity against the test fungi and was identified as 1,3,8-trihydroxy-6-methylanthracene-9,10-dione based on its IR, UV-V and 1^H NMR spectra. 展开更多
关键词 1 3 8-trihydroxy-6-methylanthracene-9 10-dione antifungal activity medicinal plants Rumex cyprius Murb.
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Evaluation of antioxidant activity of marine Agrobacterium SP. 1202
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作者 YAN Xue-fen HUANG Dan-hong ZHENG Zhong-hui SONG Si-yang ZHANG Lian-ru 《Journal of Life Sciences》 2008年第5期29-33,共5页
The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water ... The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water (WtF) from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence (CL) assay and hydroxyl free radical-initiated chemiluminescence (CL) assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent. 展开更多
关键词 antioxidant activities DPPH (1 1-Diphenyl-2-picryl-hydrazyl) CHEMILUMINESCENCE
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