A novel antibacterial biomimetic porous titanium implant with good osseointegration was prepared by freeze-casting and thermal oxidation.Bone integration properties of the porous titanium implant were evaluated by cel...A novel antibacterial biomimetic porous titanium implant with good osseointegration was prepared by freeze-casting and thermal oxidation.Bone integration properties of the porous titanium implant were evaluated by cell proliferation assay,alkaline phosphatase activity assay,X-ray examination and hard bone tissue biopsy.The in vitro cell proliferation and the level of differentiation of the group with a modified nano-porous implant surface were significantly higher than those in the group without surface modification and the dense titanium control group(P<0.05).In vivo,bone growth and osteogenesis were found in the experimental groups with modified and unmodified porous titanium implants;osteoblasts in the modified group had more mature differentiation in the pores compared to the unmodified group.Such implants can form solid,biologically compatible bone grafts with bone tissues,exhibiting good osseointegration.展开更多
To design and synthesize neamine analogues modified at 5 position offing Ⅱ, which could improve the binding affinity of aminoglycosides to 16S RNA. Started from neomycin B, modified neamine analogues were synthesized...To design and synthesize neamine analogues modified at 5 position offing Ⅱ, which could improve the binding affinity of aminoglycosides to 16S RNA. Started from neomycin B, modified neamine analogues were synthesized through organic reactions such as hydrolysis, protection, nucleophilic substitution, deprotection and reduction. The interaction of the target compounds with A-site RNA in E. coli. ribosome （16S RNA） was determined by surface plasmon resonance （SPR）, respectively. Six target compounds were synthesized. Some of them showed antibacterial activities and enhanced affinity to 16S RNA at 10^-3M in vitro. Introduction amino or aliphatic amino group at 5 position offing Ⅱ in neamine would maintained antibacterial activities as well as increase binding affinity to 16S RNA. Furthermore, there is almost no influence on the stability of drug/16S RNA complex by inverting the configuration of 5-hydroxyl group at ring Ⅱ.展开更多
To make further investigation of the IgE antibodyrepertoire in Trichosanthin (TCS) allergic responses, amurine IgE phage surface display library was constructed(3.0×105 independent clones). We first constructed t...To make further investigation of the IgE antibodyrepertoire in Trichosanthin (TCS) allergic responses, amurine IgE phage surface display library was constructed(3.0×105 independent clones). We first constructed theVe cDNA library (4.6×105 independent clones) and VκcDNA library (3.0×105 independent clones). Then, theVε and Vκ gene segments were amplified from both libraries by PCR respectively, and assembled into Fab fragment by SOE PCR. The phage library containing Fabs wasthus constructed. The diversity of Vε from this library wasanalyzed and proved. Fab clones with high specificity toTCS have been screened out.展开更多
基金Projects (51290295,51305464) supported by the National Natural Science Foundation of ChinaProject (2016JJ6156) supported by the Natural Science Foundation of Hunan Province,China+1 种基金Project (2016JC2064) supported by Key Research and Development Project of Hunan Province,ChinaProject (20130162120094) supported by Specialized Research Fund for the Doctoral Program of Higher Education,China
文摘A novel antibacterial biomimetic porous titanium implant with good osseointegration was prepared by freeze-casting and thermal oxidation.Bone integration properties of the porous titanium implant were evaluated by cell proliferation assay,alkaline phosphatase activity assay,X-ray examination and hard bone tissue biopsy.The in vitro cell proliferation and the level of differentiation of the group with a modified nano-porous implant surface were significantly higher than those in the group without surface modification and the dense titanium control group(P<0.05).In vivo,bone growth and osteogenesis were found in the experimental groups with modified and unmodified porous titanium implants;osteoblasts in the modified group had more mature differentiation in the pores compared to the unmodified group.Such implants can form solid,biologically compatible bone grafts with bone tissues,exhibiting good osseointegration.
基金National Natural Science Foundation of China(Grant No.20332010)the Ministry of Science and Technology of China(Grant No.2005BA711A04).
文摘To design and synthesize neamine analogues modified at 5 position offing Ⅱ, which could improve the binding affinity of aminoglycosides to 16S RNA. Started from neomycin B, modified neamine analogues were synthesized through organic reactions such as hydrolysis, protection, nucleophilic substitution, deprotection and reduction. The interaction of the target compounds with A-site RNA in E. coli. ribosome （16S RNA） was determined by surface plasmon resonance （SPR）, respectively. Six target compounds were synthesized. Some of them showed antibacterial activities and enhanced affinity to 16S RNA at 10^-3M in vitro. Introduction amino or aliphatic amino group at 5 position offing Ⅱ in neamine would maintained antibacterial activities as well as increase binding affinity to 16S RNA. Furthermore, there is almost no influence on the stability of drug/16S RNA complex by inverting the configuration of 5-hydroxyl group at ring Ⅱ.
文摘To make further investigation of the IgE antibodyrepertoire in Trichosanthin (TCS) allergic responses, amurine IgE phage surface display library was constructed(3.0×105 independent clones). We first constructed theVe cDNA library (4.6×105 independent clones) and VκcDNA library (3.0×105 independent clones). Then, theVε and Vκ gene segments were amplified from both libraries by PCR respectively, and assembled into Fab fragment by SOE PCR. The phage library containing Fabs wasthus constructed. The diversity of Vε from this library wasanalyzed and proved. Fab clones with high specificity toTCS have been screened out.
