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多糖的研究进展 被引量:24
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作者 丁一芳 李连闯 赵玺 《黑龙江医药》 CAS 2006年第2期123-126,共4页
多糖类成分广泛存在于中草药中,具有多种生理活性,主要为增强免疫,抗肿瘤、抗衰老、抗病毒,保护心脏、肝脏、肾脏等。本文对多糖的药理作用作一简要综述。
关键词 免疲作用 多糖 抗肿瘤 抗表老 抗病毒
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Related gene expressions in anti-keratinocyte aging induced by Ganoderma lucidum polysaccharides 被引量:7
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作者 Xie Shaoqiong Liao Wanqing +1 位作者 Yao Zhirong Wang Zhidong 《Journal of Medical Colleges of PLA(China)》 CAS 2008年第3期167-175,共9页
Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes ... Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes lines were cultured and treated with the polysaccharides.The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription.The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip.The images were scanned and analyzed with special software.The scan data were analyzed with software and checked by real time PCR.Results:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones down-regulated.It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging.Two ways are anastomotic.Conclusion:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging.It shows that the results of gene array reliable by real time PCR. 展开更多
关键词 Ganoderma lucidum polysaccharides Skin aging Gene chip KERATINOCYTE
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Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice 被引量:5
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作者 Shiyu Dai Tao Zhang +2 位作者 Yanfang Zhang Hualin Wang Fei Deng 《Virologica Sinica》 SCIE CAS CSCD 2018年第3期213-226,共14页
The newly emerged mosquito-borne Zika virus(ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. Several ZIKV vaccine candidates have been proposed, including in... The newly emerged mosquito-borne Zika virus(ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. Several ZIKV vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, DNA and RNA vaccines. These have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. Virus-like particles(VLPs) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies. In this study, we used a new strategy to produce ZIKV VLPs based on the baculovirus expression system and demonstrated the feasibility of their use as a vaccine candidate. The pre-membrane(prM) and envelope(E) proteins were co-expressed in insect cells and selfassembled into particles similar to ZIKV. We found that the ZIKV VLPs could be quickly and easily prepared in large quantities using this system. The VLPs were shown to have good immunogenicity in immunized mice, as they stimulated high levels of virus neutralizing antibody titers, ZIKV-specific IgG titers and potent memory T cell responses. Thus, the baculovirus-based ZIKV VLP vaccine is a safe, effective and economical vaccine candidate for use against ZIKV. 展开更多
关键词 ZIKV Baculovirus expression system. Virus-like particles (VLPs) Neutralizing antibodies
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