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国外水稻新品种(系)对我国不同稻瘟病菌的抗谱分析
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作者 袁筱萍 魏兴华 +3 位作者 徐群 王一平 余汉勇 王彩红 《中国稻米》 北大核心 2016年第3期17-20,共4页
选用19个不同来源的稻瘟病菌株,对577份国外引进水稻新品种(系)进行不同稻瘟病菌的抗谱分析。研究结果表明,其中有11份品种(系)对19个参测菌株均具有抗性,1份品种(系)对19个参测菌株均为感病;19个参测菌株中,CH187、CH171的致病性最强,... 选用19个不同来源的稻瘟病菌株,对577份国外引进水稻新品种(系)进行不同稻瘟病菌的抗谱分析。研究结果表明,其中有11份品种(系)对19个参测菌株均具有抗性,1份品种(系)对19个参测菌株均为感病;19个参测菌株中,CH187、CH171的致病性最强,而CH362、CH154的致病性最弱;籼稻较粳稻表现出更强的稻瘟病抗性。 展开更多
关键词 国外引种 稻瘟病菌 抗谱分析
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普通小麦中来自黑麦的抗白粉病Pm20基因的抗谱分析和AFLP定位 被引量:7
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作者 王新望 王军丽 +4 位作者 段霞瑜 周文娟 盛宝钦 朱立煌 张文俊 《科学通报》 EI CAS CSCD 北大核心 2001年第8期666-669,共4页
用24个在我国具有代表性的小麦白粉菌已知毒性菌株,对两个不同来源的小麦一黑麦6BS_6RL易位系进行了抗病性鉴定,表明 Pm20基因已发生抗谱分化.以这两个材料为亲本配制 TAM104R×TAM104S杂交组合获得... 用24个在我国具有代表性的小麦白粉菌已知毒性菌株,对两个不同来源的小麦一黑麦6BS_6RL易位系进行了抗病性鉴定,表明 Pm20基因已发生抗谱分化.以这两个材料为亲本配制 TAM104R×TAM104S杂交组合获得 F2分离群体,对亲本和 F2分离群体进行 AFLP分析.亲本 DNA经 PstⅠ/TaqⅠ双酶切消化,并接上各自的接头,然后以其接头为基础加1个或3个碱基,分别经过两次PCR扩增,结果 16对 PstⅠ/TaqⅠ引物组合(4个 PstⅠ引物, 4个 TaqⅠ引物)PCR扩增出 2290条带,共获得 3个与该基因连锁的分子标记,分别位于 Pm20基因的一端 2.6和 11.6 cM,另一端 3.6 cM处,实现了对 Pm20基因的连锁分析. 展开更多
关键词 小麦 白粉病基因 AFLP定位 抗谱分析
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GEO卫星电离层延迟时间序列抗差谱分析 被引量:1
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作者 任夏 杨元喜 《大地测量与地球动力学》 CSCD 北大核心 2014年第2期115-119,共5页
用北斗GEO卫星的观测数据,通过双频电离层改正算法获取卫星约一天内的电离层延迟数据。利用抗差谱分析方法,分离电离层延迟时间序列中的长期项、周期项和残差项,以控制异常误差对周期项的影响。计算表明,电离层时间序列谱分析拟合后的... 用北斗GEO卫星的观测数据,通过双频电离层改正算法获取卫星约一天内的电离层延迟数据。利用抗差谱分析方法,分离电离层延迟时间序列中的长期项、周期项和残差项,以控制异常误差对周期项的影响。计算表明,电离层时间序列谱分析拟合后的残差仍然受有色噪声的影响,因此,基于AR模型对残差项进行有色噪声拟合。结果表明,每天的电离层延迟不仅表现出趋势项、周期项,而且含有有色噪声。经过分段多项式、周期函数和有色噪声拟合后,电离层延迟剩余误差表现出明显的白噪声现象,证明了针对GEO卫星电离层建模的有效性。 展开更多
关键词 电离层延迟 时间序列 谱分析 有色噪声 AR模型
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地心运动对大地坐标的影响及其抗差谱分析
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作者 郭海荣 焦文海 《解放军测绘研究所学报》 2002年第3期12-15,共4页
为了明晰地分析地心运动对地面点的水平分量和垂直分量的影响规律,本文首先将地心运动时间序列从空间直角坐标转换到大地坐标,而后用抗差谱分析进行了分析,计算结果表明,地心运动在水平方向和径向都有比较明显的主周期分量,地质质... 为了明晰地分析地心运动对地面点的水平分量和垂直分量的影响规律,本文首先将地心运动时间序列从空间直角坐标转换到大地坐标,而后用抗差谱分析进行了分析,计算结果表明,地心运动在水平方向和径向都有比较明显的主周期分量,地质质心存在明显的长期北移倾向,地心运动还导致地面点在水平方向有顺时针方向的长期运动。 展开更多
关键词 地心运动 谱分析 时间序列 地球质心 空间直角坐标 大地坐标
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抗真菌菌株JW-725的分离、鉴定及发酵产物性质的初步分析 被引量:12
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作者 伍明俊 金丹 +2 位作者 李晖 陈金瑞 刘成君 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2003年第5期945-948,共4页
JW 725菌株是从西藏类乌齐协马山口高山草甸的土壤中分离得到的一株细菌.经初步鉴定为多粘芽孢杆菌(Bacilluspolymyxa).该菌株的发酵液经抗菌谱分析具很强的抗真菌活性,尤其是对柑橘青霉菌(Penicilliumitalicum)的抗性很高,且对革兰氏... JW 725菌株是从西藏类乌齐协马山口高山草甸的土壤中分离得到的一株细菌.经初步鉴定为多粘芽孢杆菌(Bacilluspolymyxa).该菌株的发酵液经抗菌谱分析具很强的抗真菌活性,尤其是对柑橘青霉菌(Penicilliumitalicum)的抗性很高,且对革兰氏阳性菌如金黄色葡萄球菌(Staphylococcusaureus)也有一定的抗性作用. 展开更多
关键词 生素 多粘芽孢杆菌 谱分析
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水稻抗白叶枯病新基因Xa32(t)的鉴定和初步定位 被引量:41
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作者 郑崇珂 王春连 +2 位作者 于元杰 梁云涛 赵开军 《作物学报》 CAS CSCD 北大核心 2009年第7期1173-1180,共8页
通过多菌系接种鉴定及抗谱分析,并与目前国际上已知抗白叶枯病基因比较,证明在水稻抗源C4064中含有一个新的抗白叶枯病基因,暂命名为Xa32(t)。应用分离集团分析法(BSA),借助SSR和EST等分子标记,对该基因进行了分子标记定位。通过对F2分... 通过多菌系接种鉴定及抗谱分析,并与目前国际上已知抗白叶枯病基因比较,证明在水稻抗源C4064中含有一个新的抗白叶枯病基因,暂命名为Xa32(t)。应用分离集团分析法(BSA),借助SSR和EST等分子标记,对该基因进行了分子标记定位。通过对F2分离群体及F3家系单株进行遗传连锁性检测,发现6个位于水稻第11染色体长臂末端的分子标记RM27256、RM27274、RM2064、ZCK24、RM6293和RM5926与Xa32(t)基因连锁。它们与Xa32(t)基因间的遗传距离分别为2.1、1.0、1.0、0.5、1.5和2.6cM。其中标记RM6293和RM5926位于染色体近端粒一侧,其他4个标记RM27256、RM27274、RM2064和ZCK24位于基因的另一侧。将Xa32(t)定位在水稻第11染色体长臂末端2.0cM范围内。 展开更多
关键词 水稻白叶枯病 抗谱分析 Xa32(t) 分子标记定位
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卫星导航系统入站干扰信号的综合分析
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作者 张婷 《现代导航》 2013年第1期11-13,共3页
本文首先介绍了在使用GEO卫星组网的某卫星导航系统中卫星入站干扰信号的特征,然后结合2012年3月2日~3月9日一周的入站干扰电平数据,采用抗差谱分析方法进行了详细分析。结果表明:入站干扰信号电平含有明显的周期变化,主周期是2.67天、... 本文首先介绍了在使用GEO卫星组网的某卫星导航系统中卫星入站干扰信号的特征,然后结合2012年3月2日~3月9日一周的入站干扰电平数据,采用抗差谱分析方法进行了详细分析。结果表明:入站干扰信号电平含有明显的周期变化,主周期是2.67天、1.52天、1.07天、0.82天和0.50天的周期变化,其中2.67天和1.52天周期变化的贡献相对较大。 展开更多
关键词 GEO 入站信号 干扰信号电平 谱分析
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Anti-ultraviolet aging tests of asphalts adapting to environment in Tibetan Plateau of China 被引量:15
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作者 廖公云 黄晓明 巴桑顿珠 《Journal of Southeast University(English Edition)》 EI CAS 2008年第4期503-507,共5页
Ultraviolet (UV) aging is one of the main factors which cause premature damage of asphalt pavements in the Tibetan Plateau, China. According to the measured levels of UV radiation, aging tests of styrene-butadiene r... Ultraviolet (UV) aging is one of the main factors which cause premature damage of asphalt pavements in the Tibetan Plateau, China. According to the measured levels of UV radiation, aging tests of styrene-butadiene rubber (SBR) asphalts with different contents of three anti-UV-aging agents including nano-TiO2, CeO2 and carbon black are performed. Common indices, which include retained penetration after thin film oven tests (TFOT) and softening point, and strategic highway research program (SHRP) indices of aged asphalts are evaluated. Infrared absorption spectral analysis is performed on asphalt specimens with 0. 8% carbon black which have been aged for different aging times (500, 1000 and 1 500 h). By grey incidence analysis, the optimal contents of anti-UV-aging agents are determined. The results show that TiO2 and CeO2 are not only good UV absorbing or shielding agents, but also strong oxidants. Carbon black is a good anti-UV-aging agent, and its optimal content is about 0. 8% of asphalt weight. UV aging of asphalt mainly occurs in the early stages of aging. The longer the aging time, the more severe the aging of asphalt. 展开更多
关键词 ultraviolet (UV) aging agent carbon black infrared absorption spectral analysis grey incidence analysis
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Colorectal carcinoma-associated antigen Ca-Hb3 detected by one-dimensional SDS-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry
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作者 Shuo Sun Feng-Jie Guo Yong-Qing Tong Jian-Gao Zhu Guan-Cheng Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第10期1588-1591,共4页
AIM: To comprehensively identify the proteins of tumor relative antigen Ca-Hb3 recognized by colorectal carcinoma monoclonal antibody Hb3. METHODS: Ca-Hb3 was isolated by SDS-polyacrylamide gel electrophoresis (PAGE) ... AIM: To comprehensively identify the proteins of tumor relative antigen Ca-Hb3 recognized by colorectal carcinoma monoclonal antibody Hb3. METHODS: Ca-Hb3 was isolated by SDS-polyacrylamide gel electrophoresis (PAGE) followed by digestion with trypsin. Trypsin peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS). The proteins identified by mass spectrometry were analyzed using bioinformatics. RESULTS: Ca-Hb3 was identified as a CKAP4-like protein by Nano HPLC tandem mass spectrometry analysis. The molecular weight of CKAP4-like protein was 62.02 kDa, including one hydrophobic region, one transmembrane domain, five coiled coils, four glycosylation sites and forty-nine phosphorylation sites. CKAP4-like protein had a high homogeneity with DeltaNp63α. The characteristic expression of DeltaNp63α that is considered a potential oncogene in the isoforms of p63 was similar to that of Ca-Hb3. CONCLUSION: Ca-Hb3 is probably a CKAP4-like protein, belonging to DeltaNp63α isoform of p63 family. 展开更多
关键词 Colorectal neoplasm Monoclonal antibody ANTIGEN Mass spectrometry P63
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Anti-inflammation Activities of Essential Oil and Anti-microbial Activities Of Ethanol Extraction from China's Rosemary 被引量:1
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作者 Jingbo ZHOU Ruqiang HUANG +1 位作者 Jihong HUANG Junwei FENG 《Agricultural Science & Technology》 CAS 2015年第5期1034-1037,1047,共5页
Rosemary(Rosmarius officinalis L.), an endemic plant species in south region of China, is traditionally used as a spice. In this research, the anti-inflammatory activities of essential oil and the antibacterial activi... Rosemary(Rosmarius officinalis L.), an endemic plant species in south region of China, is traditionally used as a spice. In this research, the anti-inflammatory activities of essential oil and the antibacterial activities of ethanol extraction were determined, respectively. Results showed that based on the GC-MS analysis there were 35 kinds of active ingredients in the essential oil in totally, mainly including D-limonene(24.158 ml/L), α-Pinene(23.325 ml/L), Camphor(9.855 ml/L),Camphene(7.076 ml/L), Verbenone(6.685 ml/L), Borneol(5.580 ml/L), etc. The LCUV determination indicated that the main components in the ethanol extractionwere rosmarinic acid(3 910 mg/kg) and carnosic acid(2 970 mg/kg). By mice peritoneal macrophage phagocytosis of chicken erythrocytes experiment, the essential oil of rosemary was shown having a significant role in anti-inflammation. And the ethanol extraction had broad-spectrum antibacterial effects, but had no effect on mold by the agar diffusion method of 8 bacteria. As a result, both rosemary essential oil and ethanol extraction had good potential medicinal values. 展开更多
关键词 China's rosemary Essential oil EXTRACTION Anti-inflammation activity Anti-microbial activity
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Improvement in Oxidative Stability of Soybean Oil by Mango Kernel Extracts
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作者 Ambati Padmaja Nunna B. L. Prasad 《Journal of Food Science and Engineering》 2012年第2期95-105,共11页
This work was undertaken to explore the potential of fruit waste materials as sources of natural antioxidants. Kernels of four varieties of mangoes of Indian origin were studied. Kernels were extracted with five diffe... This work was undertaken to explore the potential of fruit waste materials as sources of natural antioxidants. Kernels of four varieties of mangoes of Indian origin were studied. Kernels were extracted with five different solvents, tested for extraction efficiency and total phenolic content (TPC). As the methanol proved to be the best solvent, it was used for further analysis. The methanolic extracts of kernels had reducing power (RP) and scavenged 2, 2 diphenyl-l-picryl hydrazyl (DPPH) radical, which is related to their antioxidant activity (AA). When analyzed in HPLC, the extracts showed the presence of phenolic compounds. The extracts had the higher capacity to reduce the formation of peroxides and slow down the rate of oxidation than butylated hydroxyl toluene (BHT) in refined, bleached deodorized soybean oil (RBD SBO). Based on the results obtained mango kernels are potential source of natural antioxidants owing to their antioxidant activity. 展开更多
关键词 Mango kernels soybean oil (RBD SBO) Gallic acid 2 2 diphenyl-l-picryl hydrazyl (DPPH) activity
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Seismic Assessment of Asymmetric Structures Behaviour by Using Static Non-linear Analysis
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作者 Ivana Marino Luigi Petti 《Journal of Civil Engineering and Architecture》 2011年第5期402-411,共10页
The goal of this work is to investigate the seismic behaviour of plan-asymmetric structures by considering the least seismic-resistant directions and the spatial features of the seismic event. New tools for the analys... The goal of this work is to investigate the seismic behaviour of plan-asymmetric structures by considering the least seismic-resistant directions and the spatial features of the seismic event. New tools for the analysis of the seismic behaviour of plan-asymmetric structures are herein presented and the concepts of "Polar Spectrum" and limit domains are discussed. In particular, the capacity of the structure is described by using the limit domains based on the Non Linear Static Procedures, while the seismic demand is analysed by introducing a new representation of the spectral response. This representation is based on the construction of a spectral surface obtained by the spectral seismic response for different in-plan directions and the in-plan projection of this surface is herein defined "Polar Spectrum". The obtained results for two benchmark structures, verified by means of non-linear incremental dynamic analyses, have pointed out that non-linear static analyses, carried out for different in-plan directions of the incoming seismic action, have allowed us to accurately evaluate the least seismic resistant directions. 展开更多
关键词 Plan-asymmetric structures non-linear analyses polar spectrum.
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A portable single-cell analysis system integrating hydrodynamic trapping with broadband impedance spectroscopy
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作者 TANG WenLai TANG DeZhi +2 位作者 NI ZhongHua XIANG Nan YI Hong 《Science China(Technological Sciences)》 SCIE EI CAS CSCD 2017年第11期1707-1715,共9页
In this paper, we present a portable single-cell analysis system with the hydrodynamic cell trapping and the broadband electrical impedance spectroscopy (EIS). Using the least flow resistance path principle, the hyd... In this paper, we present a portable single-cell analysis system with the hydrodynamic cell trapping and the broadband electrical impedance spectroscopy (EIS). Using the least flow resistance path principle, the hydrodynamic cell trapping in serpentine arrays can be carried out in a deterministic and automatic manner without the assistance of any external fields. The experimental results show that a cell trap rate of higher than 95% can be easily achieved in our ceil trapping microdevices. Using the maximum length sequences (MLS) technique, our home-made EIS is capable of measuring the impedance spectrum ranging from 1.953 kHz to 1 MHz in approximately 0.5 ms. Finally, on the basis of the developed single-cell analysis system, we precisely monitor the trapping process of human breast tumor cells (MCF-7 cells) according to the changes of electrical impedance. The MCF-7 cells with different trapping conditions or sizes can also be clearly distinguished through the impedance signals. Our portable single-cell analysis system may provide a promising tool to monitor single cells for long periods of time or to discriminate cell types. 展开更多
关键词 single-cell analysis hydrodynamic cell trapping broadband impedance spectroscopy maximum length sequences
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