目的:天智颗粒联合西药治疗血管性认知功能障碍的临床疗效。方法:将107例我科室住院的血管性认知功能障碍患者按随机数字表法随机分为两组。对照组50例给予阿斯匹林肠溶片0.1 g,1 d 1次,口服;阿托伐他汀钙片20 mg,每晚1次,口服。治疗组5...目的:天智颗粒联合西药治疗血管性认知功能障碍的临床疗效。方法:将107例我科室住院的血管性认知功能障碍患者按随机数字表法随机分为两组。对照组50例给予阿斯匹林肠溶片0.1 g,1 d 1次,口服;阿托伐他汀钙片20 mg,每晚1次,口服。治疗组57例在对照组治疗基础上加服天智颗粒(天麻、钩藤、石决明、杜仲、桑寄生、茯神、首乌藤、槐花、栀子、黄芩、川牛膝、益母草等),1次5 g,1 d 3次;盐酸多奈哌齐片,1次5 mg,1 d 1次,晚间口服。两组均以8周为1个疗程,共治疗1个疗程。结果:治疗组显效10例,有效39例,无效8例,有效率占86.1%;对照组显效5例,有效25例,无效20例,有效率占60.0%。两组对比,差别有统计学意义(P<0.05)。结论:天智颗粒联合西药治疗血管性认知功能障碍,疗效显著。展开更多
[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua ...[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua No.11 as materials,the changes of rice seedlings under the treatment conditions of Cd,Cd＋CAT inhibitor,Cd＋APX inhibitor were studied.[Result] Under Cd stress,inhibition of CAT activity caused the significant inhibition on the growth of aerial parts,decreased the number of adventitious roots and lateral roots,but it can significant promote the elongation growth of adventitious roots and lateral roots.Moreover,the length of the first lateral root from root tip on the primary roots and adventitious roots was also increased than control.When APX activity was inhibited,the growth changes of rice were similar with that treated by CAT inhibitor.[Conclusion] CAT and APX may play important roles in the regulation of rice root system growth in both non-stress and Cd-stressed rice展开更多
[Objective] This study was conducted to investigate the regulation of heat shock factor AtHsfA1a on ascorbate peroxidase under heat stress in Arabidopsis thaliana. [Method] After heat stress treatment on transgenetic ...[Objective] This study was conducted to investigate the regulation of heat shock factor AtHsfA1a on ascorbate peroxidase under heat stress in Arabidopsis thaliana. [Method] After heat stress treatment on transgenetic A. thaliana with silenced endogenetic AtHsfA1a gene and wild A. thaliana plants as materials, the change in activity of APX enzyme was analyzed by spectrophotometry, the expression level of APX gene was investigated by real-time fluorescent quantitative PCR, and the binding condition of AtHsfAla with the promoter region of APX gene was analyzed by chromatin immunoprecipitation assay. [Result] The activity and mRNA level of APX in plants with silenced endogenetic AtHsfAla gene were higher than those in wild plants. Fragments of the promoter region of APX gene were not screened from the plants with silenced endogenetic AtHsfA1a gene, but found in wild plants. [Conclusion] This study provides a theoretical basis for the understanding of the important role of AtHsfAla in resistance to stress in plant, and is of great significance to the revealing of mechanism of resistance to stress in plant.展开更多
By pot experiment under artificially simulated water stress conditions, soluble protein content, MDA content and SOD, POD, CAT and APX activities in Malus sieversfi leaves were determined to reveal the response mechan...By pot experiment under artificially simulated water stress conditions, soluble protein content, MDA content and SOD, POD, CAT and APX activities in Malus sieversfi leaves were determined to reveal the response mechanism of M. sieversii to changes of relative soil water content. According to the results, with the decrease of relative soil water content, MDA content in M. sieversii leaves increased by mem- brane lipid peroxidation. Cells resist water stress-induced membrane lipid peroxidation and clear the increased reactive oxygen species by improving soluble protein content and SOD, POD, CAT and APX activities. However, various enzymes were involved in the response to water stress under different moisture conditions. In addition, the results indicated that M. sieversii had a good adaptability to higher relative soil water contents.展开更多
[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T a...[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.展开更多
文摘目的:天智颗粒联合西药治疗血管性认知功能障碍的临床疗效。方法:将107例我科室住院的血管性认知功能障碍患者按随机数字表法随机分为两组。对照组50例给予阿斯匹林肠溶片0.1 g,1 d 1次,口服;阿托伐他汀钙片20 mg,每晚1次,口服。治疗组57例在对照组治疗基础上加服天智颗粒(天麻、钩藤、石决明、杜仲、桑寄生、茯神、首乌藤、槐花、栀子、黄芩、川牛膝、益母草等),1次5 g,1 d 3次;盐酸多奈哌齐片,1次5 mg,1 d 1次,晚间口服。两组均以8周为1个疗程,共治疗1个疗程。结果:治疗组显效10例,有效39例,无效8例,有效率占86.1%;对照组显效5例,有效25例,无效20例,有效率占60.0%。两组对比,差别有统计学意义(P<0.05)。结论:天智颗粒联合西药治疗血管性认知功能障碍,疗效显著。
基金Supported by National Natural Science Foundation of China(30671126)~~
文摘[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua No.11 as materials,the changes of rice seedlings under the treatment conditions of Cd,Cd＋CAT inhibitor,Cd＋APX inhibitor were studied.[Result] Under Cd stress,inhibition of CAT activity caused the significant inhibition on the growth of aerial parts,decreased the number of adventitious roots and lateral roots,but it can significant promote the elongation growth of adventitious roots and lateral roots.Moreover,the length of the first lateral root from root tip on the primary roots and adventitious roots was also increased than control.When APX activity was inhibited,the growth changes of rice were similar with that treated by CAT inhibitor.[Conclusion] CAT and APX may play important roles in the regulation of rice root system growth in both non-stress and Cd-stressed rice
文摘[Objective] This study was conducted to investigate the regulation of heat shock factor AtHsfA1a on ascorbate peroxidase under heat stress in Arabidopsis thaliana. [Method] After heat stress treatment on transgenetic A. thaliana with silenced endogenetic AtHsfA1a gene and wild A. thaliana plants as materials, the change in activity of APX enzyme was analyzed by spectrophotometry, the expression level of APX gene was investigated by real-time fluorescent quantitative PCR, and the binding condition of AtHsfAla with the promoter region of APX gene was analyzed by chromatin immunoprecipitation assay. [Result] The activity and mRNA level of APX in plants with silenced endogenetic AtHsfAla gene were higher than those in wild plants. Fragments of the promoter region of APX gene were not screened from the plants with silenced endogenetic AtHsfA1a gene, but found in wild plants. [Conclusion] This study provides a theoretical basis for the understanding of the important role of AtHsfAla in resistance to stress in plant, and is of great significance to the revealing of mechanism of resistance to stress in plant.
基金Supported by Science and Technology Innovation Project of Ji'nan City "Identification of Stress-resistant Malus sieversii Germplasm Resources and Screening of Stressresistance Functional Genes"(201401125)~~
文摘By pot experiment under artificially simulated water stress conditions, soluble protein content, MDA content and SOD, POD, CAT and APX activities in Malus sieversfi leaves were determined to reveal the response mechanism of M. sieversii to changes of relative soil water content. According to the results, with the decrease of relative soil water content, MDA content in M. sieversii leaves increased by mem- brane lipid peroxidation. Cells resist water stress-induced membrane lipid peroxidation and clear the increased reactive oxygen species by improving soluble protein content and SOD, POD, CAT and APX activities. However, various enzymes were involved in the response to water stress under different moisture conditions. In addition, the results indicated that M. sieversii had a good adaptability to higher relative soil water contents.
基金Supported by Agricultural Research Project of Guizhou Province([2010]No.3045)~~
文摘[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.
