促分裂原活化蛋白激酶(Mitogen Actived Protein Kinase,MAPK)是一种蛋白激酶,蛋白激酶又称蛋白磷酸化酶,其可通过将ATP上的磷酸基团转移到底物蛋白质氨基酸残基上,来催化底物蛋白质磷酸化。MAPK级联途径广泛存在于真核生物中。植物中的...促分裂原活化蛋白激酶(Mitogen Actived Protein Kinase,MAPK)是一种蛋白激酶,蛋白激酶又称蛋白磷酸化酶,其可通过将ATP上的磷酸基团转移到底物蛋白质氨基酸残基上,来催化底物蛋白质磷酸化。MAPK级联途径广泛存在于真核生物中。植物中的MAPK级联途径与动物和酵母类似,都包括MAPKKK、MAPKK和MAPK 3种蛋白激酶。大量的研究表明,植物中的MAPK级联途径不仅能被多种生物与非生物胁迫所激活,同时也参与激素信号转导以及植物的生长发育进程。文章就植物中MAPK级联途径及其功能的研究情况进行了综述,并展望了相关研究的发展趋势。展开更多
Effects of dietary supplementation of chitosan-oligosaccharides （COS） on the growth performance, immune response, stress resistance, and disease resistance of juvenile rainbow trout Oncorhynchus mykiss were studied....Effects of dietary supplementation of chitosan-oligosaccharides （COS） on the growth performance, immune response, stress resistance, and disease resistance of juvenile rainbow trout Oncorhynchus mykiss were studied. Four experimental diets containing 0, 20, 40, or 60 mg/kg COS （COSO, COS20, COS40, and COS60, respectively） were fed to juvenile rainbow trout （initial weight = 5.2 ± 0.3 g） for 8 weeks. By the end of the feeding trial, representative groups of fish from each dietary treatment were challenged with stressor （30 see air exposure） and pathogen exposure （intraperitoneal injection with Aeromonas hydrophila ）. Results showed that supplementation of COS in diets did not affect production performance and body composition of rainbow trout. However, fish fed the COS40 diet demonstrated improved phagocytic activities, respiratory burst activities and decreased serum cortisol level. Additionally, survival following A. hydrophila challenge was significant higher among fish fed the COS-supplemented feeds, although there was no difference based on the level of supplementation. The present study suggests that COS can be used as an immuno-stimulant in rainbow trout feeds展开更多
AIM： To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer （mCRC） with the aim of identifying prognostic markers.METHODS： The expression of 44 angiogenesis-...AIM： To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer （mCRC） with the aim of identifying prognostic markers.METHODS： The expression of 44 angiogenesis- secreted factors was measured by a novel cytokine antibody array methodology. The study evaluated vas- cular endothelial growth factor （VEGF） and its soluble vascular endothelial growth factor receptor （sVEGFR）-I protein levels by enzyme immunoassay （EIA） in a panel of 16 CRC cell lines, mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction （Q-RT-PCR） and vascular en- dothelial growth factor receptor （VEGFR）-2 expressionwas analyzed by flow cytometry.展开更多
Objective： The aim of our study was to explore the inhibitory effect of Tagalsin on murine transplanted tumour and its anti-tumour mechanisms. Methods： Animal models were established by transplanting H22 hepatoma ce...Objective： The aim of our study was to explore the inhibitory effect of Tagalsin on murine transplanted tumour and its anti-tumour mechanisms. Methods： Animal models were established by transplanting H22 hepatoma cells to the left oxter of mice, and ten days later they were randomly divided into five groups： blank control group （edible oil）, positive control group （HCFU） and Tagalsin group, including low-dose, middle-dose and high-dose group. All mice were killed 24 h after medication, during which observation was conducted concerning survival conditions, body weight changes, spleen weight and tumor weight of tumor-bearing mice; the spleen index and the tumor inhibitor rate （IR） were calculated and pathological changes of tumor-bearing mice were observed by HE dye. Apoptosis factors p53 and Survivin mRNA were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results： Tagalsin can inhibit hepatoma growth effectively without influencing spleen index and body weight, the tumor inhibitor rate （IR） of low, middle and high dose group of Tagalsin were 15.81%, 36.75% and 74.79% respectively, the tumor inhibitor rate （IR） of HCFU were 73.93%. Apoptosis cells could be found from the specimen of the positive control group and Tagalsin groups. Reverse transcription polymerase chain reaction （RT-PCR） results showed that positive control group’s and Tagalsin treatment groups’ p53 gene expression enhanced significantly and Survivin gene expression dropped comparing with blank group （P 0.05）. Conclusion： Tagalsin can inhibit growth of the H22 hepatoma cells significantly, the mechanism of anti-tumor effect may work by up-regulating p53 expression and down-regulating Survivin expression. Tagalsin may be considered as a potential candidate for chemoprevention.展开更多
文摘促分裂原活化蛋白激酶(Mitogen Actived Protein Kinase,MAPK)是一种蛋白激酶,蛋白激酶又称蛋白磷酸化酶,其可通过将ATP上的磷酸基团转移到底物蛋白质氨基酸残基上,来催化底物蛋白质磷酸化。MAPK级联途径广泛存在于真核生物中。植物中的MAPK级联途径与动物和酵母类似,都包括MAPKKK、MAPKK和MAPK 3种蛋白激酶。大量的研究表明,植物中的MAPK级联途径不仅能被多种生物与非生物胁迫所激活,同时也参与激素信号转导以及植物的生长发育进程。文章就植物中MAPK级联途径及其功能的研究情况进行了综述,并展望了相关研究的发展趋势。
基金Financial support was provided by 11th 5-year National Key Technologies R & D Program Project No.2006BAD12B06,2006BAD12B08
文摘Effects of dietary supplementation of chitosan-oligosaccharides （COS） on the growth performance, immune response, stress resistance, and disease resistance of juvenile rainbow trout Oncorhynchus mykiss were studied. Four experimental diets containing 0, 20, 40, or 60 mg/kg COS （COSO, COS20, COS40, and COS60, respectively） were fed to juvenile rainbow trout （initial weight = 5.2 ± 0.3 g） for 8 weeks. By the end of the feeding trial, representative groups of fish from each dietary treatment were challenged with stressor （30 see air exposure） and pathogen exposure （intraperitoneal injection with Aeromonas hydrophila ）. Results showed that supplementation of COS in diets did not affect production performance and body composition of rainbow trout. However, fish fed the COS40 diet demonstrated improved phagocytic activities, respiratory burst activities and decreased serum cortisol level. Additionally, survival following A. hydrophila challenge was significant higher among fish fed the COS-supplemented feeds, although there was no difference based on the level of supplementation. The present study suggests that COS can be used as an immuno-stimulant in rainbow trout feeds
基金Supported by A grant of "Department of Health,Government of Navarra,Spain (23/2009)"
文摘AIM： To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer （mCRC） with the aim of identifying prognostic markers.METHODS： The expression of 44 angiogenesis- secreted factors was measured by a novel cytokine antibody array methodology. The study evaluated vas- cular endothelial growth factor （VEGF） and its soluble vascular endothelial growth factor receptor （sVEGFR）-I protein levels by enzyme immunoassay （EIA） in a panel of 16 CRC cell lines, mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction （Q-RT-PCR） and vascular en- dothelial growth factor receptor （VEGFR）-2 expressionwas analyzed by flow cytometry.
基金Supported by grants from the National "863" Scientific Item (No.2006AA09Z446)Science and Technology of Qingdao (No. 07-2-1-8-NSH-1)
文摘Objective： The aim of our study was to explore the inhibitory effect of Tagalsin on murine transplanted tumour and its anti-tumour mechanisms. Methods： Animal models were established by transplanting H22 hepatoma cells to the left oxter of mice, and ten days later they were randomly divided into five groups： blank control group （edible oil）, positive control group （HCFU） and Tagalsin group, including low-dose, middle-dose and high-dose group. All mice were killed 24 h after medication, during which observation was conducted concerning survival conditions, body weight changes, spleen weight and tumor weight of tumor-bearing mice; the spleen index and the tumor inhibitor rate （IR） were calculated and pathological changes of tumor-bearing mice were observed by HE dye. Apoptosis factors p53 and Survivin mRNA were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results： Tagalsin can inhibit hepatoma growth effectively without influencing spleen index and body weight, the tumor inhibitor rate （IR） of low, middle and high dose group of Tagalsin were 15.81%, 36.75% and 74.79% respectively, the tumor inhibitor rate （IR） of HCFU were 73.93%. Apoptosis cells could be found from the specimen of the positive control group and Tagalsin groups. Reverse transcription polymerase chain reaction （RT-PCR） results showed that positive control group’s and Tagalsin treatment groups’ p53 gene expression enhanced significantly and Survivin gene expression dropped comparing with blank group （P 0.05）. Conclusion： Tagalsin can inhibit growth of the H22 hepatoma cells significantly, the mechanism of anti-tumor effect may work by up-regulating p53 expression and down-regulating Survivin expression. Tagalsin may be considered as a potential candidate for chemoprevention.
