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“抗酮灵”对围产期母牛血液学,血液生化学和乳成分的影响 被引量:1
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作者 肖定汉 吴发荣 《北京奶牛》 1992年第1期18-21,27,共5页
关键词 母牛 围产期 血液学 血液生化学 乳成分 抗酮灵
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“抗酮灵”对产后母牛酮病的疗效观察
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作者 肖定汉 李兰华 《北京奶牛》 1991年第3期7-12,共6页
关键词 牛病 母牛 抗酮灵 疗效
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“抗酮灵”对产后母牛酮病的预防效果
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作者 李兰华 肖定汉 《北京奶牛》 1991年第4期10-13,共4页
关键词 牛病 母牛 产后 抗酮灵 预防 代谢疾病
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Apoptosis induction of colorectal cancer cells HTL-9 in vitro by the transformed products of soybean isoflavones by Ganoderma lucidum 被引量:4
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作者 Mei-lin CUI Huan-yi YANG Guo-qing HE 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2017年第12期1101-1112,共12页
Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of... Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of Ganoderma lucidum. The resultant transformed products(TSI) contained(703.21±4.35) mg/g of genistein, with transformed rates of 96.63% and 87.82% of daidzein and genistein, respectively, and TSI also could enrich the bioactive metabolites of G. lucidum. The antitumor effects of TSI on human colorectal cancer cell line HTL-9, human breast cancer cell line MCF-7, and human immortalized gastric epithelial cell line GES-1 were also studied. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay showed that TSI could dramatically reduce the viability rates of HTL-9 cells and MCF-7 cells without detectable cytotoxicity on GES-1 normal cells when the TSI concentration was lower than 100 μg/ml. With 100 μg/ml of TSI, HTL-9 cells were arrested in the G1 phase, and late-apoptosis was primarily induced, accompanied with partial early-apoptosis. TSI could induce primarily earlyapoptosis by arresting cells in the G1 phase of MCF-7 cells. For HTL-9 cells, Western-blot and reverse-transcriptase polymerase chain reaction(RT-PCR) analysis showed that TSI(100 μg/ml) can up-regulate the expression of Bax, Caspase-3, Caspase-8, and cytochrome c(Cyto-c), indicating that TSI could induce cell apoptosis mainly through the mitochondrial pathway. In addition, the expression of p53 was up-regulated, while the expression of Survivin and nuclear factor κB(NF-κB) was down-regulated. All these results showed that TSI could induce apoptosis of HTL-9 cells by the regulation of multiple apoptosis-related genes. 展开更多
关键词 Soybean isoflavones Ganoderma lucidum TRANSFORMATION Antiproliferative activity APOPTOSIS
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