为研究水稻抗重金属的特性,构建了水稻金属硫蛋白(metallothionein,MT)原核表达载体PGEX4T-3-Os MT-1-4c,并转化到Rossetta菌株中诱导表达,得到大小为33.643 k Da的GST-Os MT-1-4c重组蛋白。将得到的沉淀用1×PBS溶解并在低温下超...为研究水稻抗重金属的特性,构建了水稻金属硫蛋白(metallothionein,MT)原核表达载体PGEX4T-3-Os MT-1-4c,并转化到Rossetta菌株中诱导表达,得到大小为33.643 k Da的GST-Os MT-1-4c重组蛋白。将得到的沉淀用1×PBS溶解并在低温下超声破碎,离心并电泳检测,然后纯化得到融合蛋白。并在重金属Cd^(2+)胁迫下诱导表达重组载体和空载体菌株,发现能表达GST-Os MT-1-4c蛋白菌株比表达GST蛋白菌株抗重金属的能力强。展开更多
In this paper, we describe the synthesis of a novel copper ion hapten using the copper mercaptide of penicillenic acid （CMPA） derived from penicillin. Results from tests with immune rabbits indicate that： （i） A n...In this paper, we describe the synthesis of a novel copper ion hapten using the copper mercaptide of penicillenic acid （CMPA） derived from penicillin. Results from tests with immune rabbits indicate that： （i） A new antigen synthesized with CMPA has good stability and is safe for immunizing animals with no toxic phenomena being found in animal experiments; （ii） the immunogenic antigen （CMPA-BSA） can stimulate the immune system to produce specific antibodies with high titrations, up to 150000; and （iii） antibodies in antisera showed higher affinity to OVA-GSH-CuC1 than OVA-GSH, which indicates that the antibodies have specific affinity towards copper ions. These results confirm that the novel hapten and relevant antigen for copper ion have been successfully synthesized, giving progress towards an immunoassay for copper ions in environmental and food samples.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 30821005)National High Technology Research and Development Program of China (Grant No. 2007AA10Z401)
文摘In this paper, we describe the synthesis of a novel copper ion hapten using the copper mercaptide of penicillenic acid （CMPA） derived from penicillin. Results from tests with immune rabbits indicate that： （i） A new antigen synthesized with CMPA has good stability and is safe for immunizing animals with no toxic phenomena being found in animal experiments; （ii） the immunogenic antigen （CMPA-BSA） can stimulate the immune system to produce specific antibodies with high titrations, up to 150000; and （iii） antibodies in antisera showed higher affinity to OVA-GSH-CuC1 than OVA-GSH, which indicates that the antibodies have specific affinity towards copper ions. These results confirm that the novel hapten and relevant antigen for copper ion have been successfully synthesized, giving progress towards an immunoassay for copper ions in environmental and food samples.
