[Objective] This study was to identify the expression of exogenous antimicrobial peptide in transgenic Houttuynia cordata Thunb. plants,and analyze their resistance to stem rot disease. [Methods] SDS-PAGE and Western ...[Objective] This study was to identify the expression of exogenous antimicrobial peptide in transgenic Houttuynia cordata Thunb. plants,and analyze their resistance to stem rot disease. [Methods] SDS-PAGE and Western blot analysis were employed to detect expression of exogenous antimicrobial peptide in transgenic H. cordata plants. Both wild type and transgenic H. cordata plants were inoculated with different concentrations of Rhizoctonia solani spores for detecting their resistance. [Results] The exogenous antimicrobial peptide was detected at translation level. The optimal parameters for detecting the resistance of transgenic H. cordata plants to R. solani was inoculation of spores at a concentration of 3×105 ind./ml and cultured for three days. The results showed that resistance of transgenic H. cordata plants to R. solani was enhanced in comparison with CKs. [Conclusion] Expression of exogenous antimicrobial peptide can enhance the resistance of transgenic H. cordata plants to stem rot disease.展开更多
Three gram negative bacteria isolated from infected koi carp, Cyprinus carpio L. and screened for sensitivity to thirty antibiotics by the Bauer disc diffusion method. Bacteria evaluated were Aeromonas hydrophila, Pse...Three gram negative bacteria isolated from infected koi carp, Cyprinus carpio L. and screened for sensitivity to thirty antibiotics by the Bauer disc diffusion method. Bacteria evaluated were Aeromonas hydrophila, Pseudomonas aeruginosa and Edwardseilla tarda. A. hydrophila was resistant to ampicilin, cephalothin, sulphatriad, cefpodoxime and cefoxitin. Highest sensitivity was recorded to Colistin methane sulphonate and Ceftriaxone. High resistance was observed for P. aeruginosa in Kanamysin, Cephalothin, Sulphatriad, Colistin methane sulphonate, Tobramysin, Nitro Furantoin, Cefpodoxime, Cefoxitin and Aztreonam. Highest sensitivity recorded with Moxifloxacin. High resistance was observed in Co-trimoxamole and higher sensitivity performed for E. tarda by Tetracyclin, Cephotaxime, Ciprofloxacin, Norfloxacin, Ofloxacin, Moxifloxacin, Gatifloxacin and Levofloxacin. Out of that highest sensitivity recorded with Moxifloxacin High sensitivity was recorded for Moxifloxacin, Ciprofloxacin, Gatifloxacin, Levofloxacin. By means of antibiotic sensitivity test highest resistance was recorded to P. aeruginosa （MAR index-0.3） and least resistant was recorded to E, tarda （MAR index- 0.033）.展开更多
基金Supported by National Natural Science Foundation of China(30772737)~~
文摘[Objective] This study was to identify the expression of exogenous antimicrobial peptide in transgenic Houttuynia cordata Thunb. plants,and analyze their resistance to stem rot disease. [Methods] SDS-PAGE and Western blot analysis were employed to detect expression of exogenous antimicrobial peptide in transgenic H. cordata plants. Both wild type and transgenic H. cordata plants were inoculated with different concentrations of Rhizoctonia solani spores for detecting their resistance. [Results] The exogenous antimicrobial peptide was detected at translation level. The optimal parameters for detecting the resistance of transgenic H. cordata plants to R. solani was inoculation of spores at a concentration of 3×105 ind./ml and cultured for three days. The results showed that resistance of transgenic H. cordata plants to R. solani was enhanced in comparison with CKs. [Conclusion] Expression of exogenous antimicrobial peptide can enhance the resistance of transgenic H. cordata plants to stem rot disease.
文摘Three gram negative bacteria isolated from infected koi carp, Cyprinus carpio L. and screened for sensitivity to thirty antibiotics by the Bauer disc diffusion method. Bacteria evaluated were Aeromonas hydrophila, Pseudomonas aeruginosa and Edwardseilla tarda. A. hydrophila was resistant to ampicilin, cephalothin, sulphatriad, cefpodoxime and cefoxitin. Highest sensitivity was recorded to Colistin methane sulphonate and Ceftriaxone. High resistance was observed for P. aeruginosa in Kanamysin, Cephalothin, Sulphatriad, Colistin methane sulphonate, Tobramysin, Nitro Furantoin, Cefpodoxime, Cefoxitin and Aztreonam. Highest sensitivity recorded with Moxifloxacin. High resistance was observed in Co-trimoxamole and higher sensitivity performed for E. tarda by Tetracyclin, Cephotaxime, Ciprofloxacin, Norfloxacin, Ofloxacin, Moxifloxacin, Gatifloxacin and Levofloxacin. Out of that highest sensitivity recorded with Moxifloxacin High sensitivity was recorded for Moxifloxacin, Ciprofloxacin, Gatifloxacin, Levofloxacin. By means of antibiotic sensitivity test highest resistance was recorded to P. aeruginosa （MAR index-0.3） and least resistant was recorded to E, tarda （MAR index- 0.033）.
