Objective: To analyze the genotypes of CCR5 △ 32,CCR2b-64I and SDF 1-3 A and mutation frequencies of allelicgenes in Chinese populations infected with HIV-1. Methods: Genome DNA from peripheral blood mononuclearcells...Objective: To analyze the genotypes of CCR5 △ 32,CCR2b-64I and SDF 1-3 A and mutation frequencies of allelicgenes in Chinese populations infected with HIV-1. Methods: Genome DNA from peripheral blood mononuclearcells (PBMCs) of 78 HIV-1 infectors was amplified bypolymerase chain reaction (PCR). CCR5, CCR2b and SDF1gene fragments were obtained from restrictive fragmentlength polymorphism (RFLP) and/or CCR△32, CCR5m303,CCR2b-64I and SDF1-3' A allelic genes' mutationalfrequencies were sequenced directly from PCR products. Results: None of CCR5△32, CCR5m303 gene mutationwere found in 78 subjects with HIV-1 infection. The allelicgene mutation frequencies of CCR2b-64I and SDF1-3'Acorresponding to 14.9-34.0% and 17.6-38.2% of 95% CI, were22.79% and 26.92% respectively. Their colony distributionconformed to the Hardy-Weinberg equilibrium. Conclusion: The HIV-1 infections found at present are allsusceptible population of CCR5△32 and CCR5m303. Thepolymorphism and frequencies of CCR5△32, CCR5m303,CCR2b-64I and SDF1-3'A alleles from Chinese HIV-1infected population were disclosed in this study for the firsttime, which is of significance for studying the geneticresistance to susceptibility to HIV-1 infection as well as AIDSdisease progression.展开更多
文摘Objective: To analyze the genotypes of CCR5 △ 32,CCR2b-64I and SDF 1-3 A and mutation frequencies of allelicgenes in Chinese populations infected with HIV-1. Methods: Genome DNA from peripheral blood mononuclearcells (PBMCs) of 78 HIV-1 infectors was amplified bypolymerase chain reaction (PCR). CCR5, CCR2b and SDF1gene fragments were obtained from restrictive fragmentlength polymorphism (RFLP) and/or CCR△32, CCR5m303,CCR2b-64I and SDF1-3' A allelic genes' mutationalfrequencies were sequenced directly from PCR products. Results: None of CCR5△32, CCR5m303 gene mutationwere found in 78 subjects with HIV-1 infection. The allelicgene mutation frequencies of CCR2b-64I and SDF1-3'Acorresponding to 14.9-34.0% and 17.6-38.2% of 95% CI, were22.79% and 26.92% respectively. Their colony distributionconformed to the Hardy-Weinberg equilibrium. Conclusion: The HIV-1 infections found at present are allsusceptible population of CCR5△32 and CCR5m303. Thepolymorphism and frequencies of CCR5△32, CCR5m303,CCR2b-64I and SDF1-3'A alleles from Chinese HIV-1infected population were disclosed in this study for the firsttime, which is of significance for studying the geneticresistance to susceptibility to HIV-1 infection as well as AIDSdisease progression.
