The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positi...The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.展开更多
Objective: To evaluate the clinical utility of testingserum anti-treponema pallidum IgM antibody in thediagnosis of syphilis patients. Methods: Seventy-two cases of syphilis were testedfor specific IgM antibody with E...Objective: To evaluate the clinical utility of testingserum anti-treponema pallidum IgM antibody in thediagnosis of syphilis patients. Methods: Seventy-two cases of syphilis were testedfor specific IgM antibody with ELISA, and the resultswere compared with RPR and TPPA. Results: The sensitivity of IgM antibody was 73.3%(11/15) in primary syphilis, 88.9% (16/18) in sec-ondary syphilis, and there was no significant differ-ence between these values (x^2=1.6363, P>0.10). Thesensitivity of IgM antibody in diagnosing latent syphi-lis was only 26.1% (6/23), much lower than the detec-tion rate in symptomatic early syphilis (x^2=17.6189, P<0.005). RPR and TPPA were both 100% sensitive inlatent and early symptomatic syphilis. Two were posi-tive for IgM in the 16 cases who had received regulartreatments 2 to 24 months before enrolled. Conclusions: Specific IgM antibody detection doesnot appear superior to RPR and TPPA in diagnosingprimary syphilis. The diagnosis of latent syphilisshould mainly rely on RPR and TPPA, since there arelow titers of IgM antibody at that stage. IgM antibodytesting alone should not be recommended for monitor-ing syphilis development or treatment efficacy. Fur-ther studies should be concerned.展开更多
检测对象为济南市2004—2006年无偿献血者163854人，均体检合格，年龄18～55岁。抗-HCV试剂购自北京金豪公司、北京万泰公司、上海科华公司，批检合格，有效期内使用，严格按说明书操作。全自动样品处理仪Microlab AT plus2，全自动酶...检测对象为济南市2004—2006年无偿献血者163854人，均体检合格，年龄18～55岁。抗-HCV试剂购自北京金豪公司、北京万泰公司、上海科华公司，批检合格，有效期内使用，严格按说明书操作。全自动样品处理仪Microlab AT plus2，全自动酶免分析系统MicrolabFAME，购自瑞士哈密顿公司。检测用ELISA方法，用不同厂家试剂进行初复检。展开更多
文摘The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.
文摘Objective: To evaluate the clinical utility of testingserum anti-treponema pallidum IgM antibody in thediagnosis of syphilis patients. Methods: Seventy-two cases of syphilis were testedfor specific IgM antibody with ELISA, and the resultswere compared with RPR and TPPA. Results: The sensitivity of IgM antibody was 73.3%(11/15) in primary syphilis, 88.9% (16/18) in sec-ondary syphilis, and there was no significant differ-ence between these values (x^2=1.6363, P>0.10). Thesensitivity of IgM antibody in diagnosing latent syphi-lis was only 26.1% (6/23), much lower than the detec-tion rate in symptomatic early syphilis (x^2=17.6189, P<0.005). RPR and TPPA were both 100% sensitive inlatent and early symptomatic syphilis. Two were posi-tive for IgM in the 16 cases who had received regulartreatments 2 to 24 months before enrolled. Conclusions: Specific IgM antibody detection doesnot appear superior to RPR and TPPA in diagnosingprimary syphilis. The diagnosis of latent syphilisshould mainly rely on RPR and TPPA, since there arelow titers of IgM antibody at that stage. IgM antibodytesting alone should not be recommended for monitor-ing syphilis development or treatment efficacy. Fur-ther studies should be concerned.
文摘检测对象为济南市2004—2006年无偿献血者163854人，均体检合格，年龄18～55岁。抗-HCV试剂购自北京金豪公司、北京万泰公司、上海科华公司，批检合格，有效期内使用，严格按说明书操作。全自动样品处理仪Microlab AT plus2，全自动酶免分析系统MicrolabFAME，购自瑞士哈密顿公司。检测用ELISA方法，用不同厂家试剂进行初复检。
