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镁离子对碱性磷酸酶折叠过程的影响 被引量:2
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作者 宋晓红 闫淑莲 +2 位作者 田晓娟 张英侠 周海梦 《首都医科大学学报》 CAS 2003年第3期221-224,共4页
对小牛肠碱性磷酸酶再折叠的机制及其影响因素进行了探讨。在 2 5℃室温下 ,以 3mol/L盐酸胍对小牛肠碱性磷酸酶 (CIP)进行了 2h的去折叠实验 ,然后再以Tris HCl缓冲液对变性体系进行 2 0倍稀释 ,观察该酶活力恢复的动力学过程并与镁离... 对小牛肠碱性磷酸酶再折叠的机制及其影响因素进行了探讨。在 2 5℃室温下 ,以 3mol/L盐酸胍对小牛肠碱性磷酸酶 (CIP)进行了 2h的去折叠实验 ,然后再以Tris HCl缓冲液对变性体系进行 2 0倍稀释 ,观察该酶活力恢复的动力学过程并与镁离子参与下的稀释复性过程进行比较。并就不同实验条件下含镁稀释或不含镁稀释 ,获得的最终酶活力、酶活力恢复水平、活力恢复速率常数及数据的相对标准偏差等指标进行了探讨。认为镁是CIP位点的特异性“效应分子” ,它与酶中有关基团的配位 ,稳定了酶活性部位的构象 。 展开更多
关键词 镁离子 碱性磷酸酶 折叠实验 CIP 缓冲溶液
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Reanalysis of Elastic Scattering of ~6Li + ^(209)Bi Reaction Using a New DensityDistribution of ~6Li Nucleus
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作者 M. Aygun 《Communications in Theoretical Physics》 SCIE CAS CSCD 2013年第7期69-72,共4页
In the present paper, the elastic scattering of6 Li + 209 Bi system is reanalyzed by using the double folding model (DFM) at energies near the Coulomb barrier (ELab=29.9 and 32.8 MeV). With this goal, a new density di... In the present paper, the elastic scattering of6 Li + 209 Bi system is reanalyzed by using the double folding model (DFM) at energies near the Coulomb barrier (ELab=29.9 and 32.8 MeV). With this goal, a new density distribution of6 Li nucleus, the no-core full configuration (NCFC) density distribution (DD), is used to obtain the real potentials in DFM calculations. The NCFC DD results are compared with the results of both gaussian shape (GS) DD and an earlier study as well as the experimental data. This comparison provides information about the similarities and differences of the models used in calculations. 展开更多
关键词 optical model microscopic approaches double folding model elastic scattering
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Folding Model Analysis of Elastic Scattering of 11B from Light,Medium,and Heavy Nuclei
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作者 M.Aygun 《Communications in Theoretical Physics》 SCIE CAS CSCD 2016年第11期531-540,共10页
The elastic scattering angular distributions of z113 projectile on light, medium, and heavy target nuclei including 7Li, 9Be, 12C, 16O, 24,25,26Mg, 27Al, 28Si, 40Ca, 58Ni, 59Co, 60Ni, 197Au, 208Pb, and 209Bi have been... The elastic scattering angular distributions of z113 projectile on light, medium, and heavy target nuclei including 7Li, 9Be, 12C, 16O, 24,25,26Mg, 27Al, 28Si, 40Ca, 58Ni, 59Co, 60Ni, 197Au, 208Pb, and 209Bi have been analyzed at various incident energies. The theoretical results have been obtained by using two different nuclear potentials within the framework of the optical model (OM). Firstly, the double folding potential for real part and the Wood-Saxon (WS) potential for imaginary part have been applied. Secondly, the calculations with double folding potential for both real and imaginary part have been performed and compared with the experimental data. It has been seen that the results are in very good agreement with the experimental data. Also, the volume integrals and cross-sections for each reaction have been obtained. Finally, a new and simple formula for the imaginary potential depth has been derived to clarify the nuclear interactions of 11B nucleus at low energy reactions. 展开更多
关键词 optical model elastic scattering
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