《全美助华联合总会新闻报》(News of United China Relief)是二战期间《时代》周刊创始人亨利·卢斯组建的"全美助华联合总会"的官方新闻报刊。该报刊主要面向美国民众宣传援华抗战,动员民间各方募捐筹款,帮助中国人民...《全美助华联合总会新闻报》(News of United China Relief)是二战期间《时代》周刊创始人亨利·卢斯组建的"全美助华联合总会"的官方新闻报刊。该报刊主要面向美国民众宣传援华抗战,动员民间各方募捐筹款,帮助中国人民抗击日本。本文认为,该报通过对中国地理景观、民俗年节、书画艺术的报道,构建了一个对中国地域、生活、艺术文化的认同场域,激发了更高层次的援华动员。展开更多
Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located...Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus production.Methods Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'UTR.Dicistronic expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,respecitvely.Results tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'UTR.Furthermore,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.展开更多
Similarly to what happens in other European countries, Portugal has been feeling the need to rethink its public state subsidy scheme to media. In this context, this paper presents an analysis of the impacts arising fr...Similarly to what happens in other European countries, Portugal has been feeling the need to rethink its public state subsidy scheme to media. In this context, this paper presents an analysis of the impacts arising from the provision of such subventions in local and regional media, questioning if the state support system is adequate to the business reality and contributing to improving it. Hence, this work reflects regional realities in the national context, being also, in a greater scale, a mirror of the models prevalent in Europe, a continent marked by significant state intervention. This study also establishes a diverse set of information, including an intensive collection of empirical data on the impact of direct incentives on the companies＇ performance, product quality, market competitiveness and pluralism of voices in the regional and local information market, based on a case study on the Portuguese state support system and the analysis of 21 local and regional media companies. In fact, the results of this research point out that, although these incentives are considered by the affected media companies as very important, having positive impacts regarding the improvement of journalistic products （such as online presence on the Intemet）, maintaining human resources and promoting the pluralism of voices; they do not seem to be adequately efficient to provide a sustainable growth in local and regional media.展开更多
This article seeks to analyze the contradictions and recommendations to China＇s insurance industry in the context of low-growth and low interest market. Under the pressure caused by the contradictions between the sus...This article seeks to analyze the contradictions and recommendations to China＇s insurance industry in the context of low-growth and low interest market. Under the pressure caused by the contradictions between the sustainable growth in the insurance premium income and the low ROI, it is very important to solve these contradictions for maintaining a sustainable and steady growth in insurance industry, at the same time, it is also favorable to play a role as a social stabilizer as well as an economic booster for insurance industry. This paper can be divided into four parts： Part I analyzes the five basic characteristics of Chinese low-growth and low-interest insurance industry; part II elaborates the principal contradictions faced by Chinese low-growth and low-interest insurance industry; part III analyzes the development environment of Chinese insurance industry; part IV proposes the recommendations to solve these contradictions.展开更多
文摘《全美助华联合总会新闻报》(News of United China Relief)是二战期间《时代》周刊创始人亨利·卢斯组建的"全美助华联合总会"的官方新闻报刊。该报刊主要面向美国民众宣传援华抗战,动员民间各方募捐筹款,帮助中国人民抗击日本。本文认为,该报通过对中国地理景观、民俗年节、书画艺术的报道,构建了一个对中国地域、生活、艺术文化的认同场域,激发了更高层次的援华动员。
文摘Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus production.Methods Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'UTR.Dicistronic expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,respecitvely.Results tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'UTR.Furthermore,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.
文摘Similarly to what happens in other European countries, Portugal has been feeling the need to rethink its public state subsidy scheme to media. In this context, this paper presents an analysis of the impacts arising from the provision of such subventions in local and regional media, questioning if the state support system is adequate to the business reality and contributing to improving it. Hence, this work reflects regional realities in the national context, being also, in a greater scale, a mirror of the models prevalent in Europe, a continent marked by significant state intervention. This study also establishes a diverse set of information, including an intensive collection of empirical data on the impact of direct incentives on the companies＇ performance, product quality, market competitiveness and pluralism of voices in the regional and local information market, based on a case study on the Portuguese state support system and the analysis of 21 local and regional media companies. In fact, the results of this research point out that, although these incentives are considered by the affected media companies as very important, having positive impacts regarding the improvement of journalistic products （such as online presence on the Intemet）, maintaining human resources and promoting the pluralism of voices; they do not seem to be adequately efficient to provide a sustainable growth in local and regional media.
文摘This article seeks to analyze the contradictions and recommendations to China＇s insurance industry in the context of low-growth and low interest market. Under the pressure caused by the contradictions between the sustainable growth in the insurance premium income and the low ROI, it is very important to solve these contradictions for maintaining a sustainable and steady growth in insurance industry, at the same time, it is also favorable to play a role as a social stabilizer as well as an economic booster for insurance industry. This paper can be divided into four parts： Part I analyzes the five basic characteristics of Chinese low-growth and low-interest insurance industry; part II elaborates the principal contradictions faced by Chinese low-growth and low-interest insurance industry; part III analyzes the development environment of Chinese insurance industry; part IV proposes the recommendations to solve these contradictions.
