[ Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method ] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form an...[ Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method ] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form and function of typical candidate gene were analyzed. [ Result] The identified snR95 box H/ACA snoRNA had conservative component and structural features of box C/D snoRNA family, possessed two more than 10 nt long rRNA antisense elements. The result revealed that the novel snoRNA is a partial counterpart of the rice Z270, named box C/D snoRNA -AthZ270. [ Conclusion ] Z270 snoRNA in Arabidopsis thalianan has different function with common snoRNA.展开更多
在1983年1月于迈阿密召开的"植物和动物分子遗传学"冬季研讨会上,以比利时 Gent 大学 MarcVan Montagu 和 Jeff Schell 为首以及孟山都公司以 Rob Frally 为首的研究小组提交了三篇文章,这标志着植物遗传工程的开始。他们把...在1983年1月于迈阿密召开的"植物和动物分子遗传学"冬季研讨会上,以比利时 Gent 大学 MarcVan Montagu 和 Jeff Schell 为首以及孟山都公司以 Rob Frally 为首的研究小组提交了三篇文章,这标志着植物遗传工程的开始。他们把根癌土壤杆菌(一种能转移部分 Ti 质粒—称之为 T-DNA—的细菌)中的脱毒 Ti 质粒转移到了植物基因组。从该 T-DNA 区除去致冠瘿病的基因而保留完整的转移机制。展开更多
The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesi...The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications.展开更多
基金Supported by Natural Science Project Establishment Subject of Jiangxi Science & Technology Normal University(KY2008ZY03 )Teaching Research Provincial Project Establishment Subject of Higher Education of Jiangxi Province (JXJG-13-29)~~
文摘[ Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method ] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form and function of typical candidate gene were analyzed. [ Result] The identified snR95 box H/ACA snoRNA had conservative component and structural features of box C/D snoRNA family, possessed two more than 10 nt long rRNA antisense elements. The result revealed that the novel snoRNA is a partial counterpart of the rice Z270, named box C/D snoRNA -AthZ270. [ Conclusion ] Z270 snoRNA in Arabidopsis thalianan has different function with common snoRNA.
文摘在1983年1月于迈阿密召开的"植物和动物分子遗传学"冬季研讨会上,以比利时 Gent 大学 MarcVan Montagu 和 Jeff Schell 为首以及孟山都公司以 Rob Frally 为首的研究小组提交了三篇文章,这标志着植物遗传工程的开始。他们把根癌土壤杆菌(一种能转移部分 Ti 质粒—称之为 T-DNA—的细菌)中的脱毒 Ti 质粒转移到了植物基因组。从该 T-DNA 区除去致冠瘿病的基因而保留完整的转移机制。
基金supported by the Chinese Academy of Sciences and China Scholarship Council(201206050103)
文摘The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications.
