隐花色素(cryptochrome,简称CRY)是与DNA光解酶氨基酸序列高度同源的黄素蛋白,但不具有光解酶活性。拟南芥的隐花色素家族中隐花色素1(CRY1)主要抑制下胚轴伸长,隐花色素2(CRY2)主要调节光周期开花,并且这两个光受体具有部分重叠的功能...隐花色素(cryptochrome,简称CRY)是与DNA光解酶氨基酸序列高度同源的黄素蛋白,但不具有光解酶活性。拟南芥的隐花色素家族中隐花色素1(CRY1)主要抑制下胚轴伸长,隐花色素2(CRY2)主要调节光周期开花,并且这两个光受体具有部分重叠的功能。cry1cry2双突变体在长日条件下比野生型晚开花。采用激活标签的方法,在cry1cry2双突变体背景下筛选到一个早开花突变体scc17-D(suppressor of cry1cry2#17-Dominant)。TAIL-PCR结果表明,scc17-D突变体的T-DNA插入在第一条染色体上的At1g25440和At1g25450两个基因之间。scc17-D突变体表现出植株矮化、叶片变小、果荚变小和子叶表皮细胞形状发生改变等性状。展开更多
采取在高盐平板上萌发的方法,对一个雌激素诱导激活型拟南芥突变体库进行了耐盐突变体的筛选,最终得到了2株稳定的耐盐突变体。本文中对其中的一株耐盐突变体,命名为stg2(salt tolerance during germination 2),进行了研究。遗传实验表...采取在高盐平板上萌发的方法,对一个雌激素诱导激活型拟南芥突变体库进行了耐盐突变体的筛选,最终得到了2株稳定的耐盐突变体。本文中对其中的一株耐盐突变体,命名为stg2(salt tolerance during germination 2),进行了研究。遗传实验表明它的耐盐特性是受雌激素诱导的,是功能获得型的耐盐突变体。本实验中还探讨了stg2突变体的筛选过程及耐盐生理特点。展开更多
Dear Editor, Forward genetic screens are commonly used as unbiased tools to isolate genes responsible for a phenotype of interest. In Arabidopsis thaliana, especially T-DNA activation tagging pop- ulations are freque...Dear Editor, Forward genetic screens are commonly used as unbiased tools to isolate genes responsible for a phenotype of interest. In Arabidopsis thaliana, especially T-DNA activation tagging pop- ulations are frequently employed. These populations are gener- ated using vectors containing multiple copies of the constitutive 35S promoters derived from cau and often result in isolation i flower mosaic virus （35S CaMV） of dominant gain-of-function alleles （Weigel et al., 2000; Nakazawa et al., 2003）. This allows the study of members of large gene families that are often func- tionally redundant and, therefore, hard to identify in loss-of- function screens. Moreover, due to the dominant nature,展开更多
文摘隐花色素(cryptochrome,简称CRY)是与DNA光解酶氨基酸序列高度同源的黄素蛋白,但不具有光解酶活性。拟南芥的隐花色素家族中隐花色素1(CRY1)主要抑制下胚轴伸长,隐花色素2(CRY2)主要调节光周期开花,并且这两个光受体具有部分重叠的功能。cry1cry2双突变体在长日条件下比野生型晚开花。采用激活标签的方法,在cry1cry2双突变体背景下筛选到一个早开花突变体scc17-D(suppressor of cry1cry2#17-Dominant)。TAIL-PCR结果表明,scc17-D突变体的T-DNA插入在第一条染色体上的At1g25440和At1g25450两个基因之间。scc17-D突变体表现出植株矮化、叶片变小、果荚变小和子叶表皮细胞形状发生改变等性状。
文摘采取在高盐平板上萌发的方法,对一个雌激素诱导激活型拟南芥突变体库进行了耐盐突变体的筛选,最终得到了2株稳定的耐盐突变体。本文中对其中的一株耐盐突变体,命名为stg2(salt tolerance during germination 2),进行了研究。遗传实验表明它的耐盐特性是受雌激素诱导的,是功能获得型的耐盐突变体。本实验中还探讨了stg2突变体的筛选过程及耐盐生理特点。
文摘Dear Editor, Forward genetic screens are commonly used as unbiased tools to isolate genes responsible for a phenotype of interest. In Arabidopsis thaliana, especially T-DNA activation tagging pop- ulations are frequently employed. These populations are gener- ated using vectors containing multiple copies of the constitutive 35S promoters derived from cau and often result in isolation i flower mosaic virus （35S CaMV） of dominant gain-of-function alleles （Weigel et al., 2000; Nakazawa et al., 2003）. This allows the study of members of large gene families that are often func- tionally redundant and, therefore, hard to identify in loss-of- function screens. Moreover, due to the dominant nature,
