The amino acid sequences of N-terminal and internal peptide of OPHC2,purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab,are determined.The full-length organphosphorus hydrolase gene ophc2 is cloned by ...The amino acid sequences of N-terminal and internal peptide of OPHC2,purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab,are determined.The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR.The ophc2 gene is 975 bp long with G+C content of 63%,comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD.The nucleotide sequence of ophc2 shows low homolo- gies with those organphosphorus hydrolase genes deposited in Gen- Bank,one of which exhibits the highest homology of 46.4% with ophc2.The organphosphorus hydrolase protein expressed in E.coli bears normal bioactivity.展开更多
Accumulating evidence has suggested that epigenetic marks including DNA methylation,small RNA and histone modification may involve hybrid vigor in plants.However,knowledge about how epigenetic marks in hybrids regulat...Accumulating evidence has suggested that epigenetic marks including DNA methylation,small RNA and histone modification may involve hybrid vigor in plants.However,knowledge about how epigenetic marks in hybrids regulate gene expression is still limited.Based on genome-wide DNA methylation landscapes of Arabidopsis thaliana Ler and C24 ecotypes and their reciprocal F1 hybrids which were obtained in our previous work,we analyzed allele-specific DNA methylation and distinguished cis-and trans-regulated DNA methylation in hybrids.Our study indicated that both cis-and trans-regulated DNA methylation played roles in hybrids,when cis-regulation played a major role in CG methylation and trans-regulation played major roles in CHG and CHH methylation.In addition,we observed correlations between trans-regulated DNA methylation and siRNA densities.Enriched siRNA regions were significantly concurrent with highly trans-regulated DNA methylation regions.Our results illustrated DNA methylation regulation patterns integrated with siRNAs in Arabidopsis hybrids,and shed light on understanding the mechanism of epigenetic reprogramming for hybrid vigor.展开更多
文摘The amino acid sequences of N-terminal and internal peptide of OPHC2,purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab,are determined.The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR.The ophc2 gene is 975 bp long with G+C content of 63%,comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD.The nucleotide sequence of ophc2 shows low homolo- gies with those organphosphorus hydrolase genes deposited in Gen- Bank,one of which exhibits the highest homology of 46.4% with ophc2.The organphosphorus hydrolase protein expressed in E.coli bears normal bioactivity.
基金supported by Ministry of Science and Technology of China(2013CBA01402)Ministry of Science and Technology of China(2012CB910900)+1 种基金Ministry of Agriculture of China(2010ZX08010-003)Peking University 985 Program
文摘Accumulating evidence has suggested that epigenetic marks including DNA methylation,small RNA and histone modification may involve hybrid vigor in plants.However,knowledge about how epigenetic marks in hybrids regulate gene expression is still limited.Based on genome-wide DNA methylation landscapes of Arabidopsis thaliana Ler and C24 ecotypes and their reciprocal F1 hybrids which were obtained in our previous work,we analyzed allele-specific DNA methylation and distinguished cis-and trans-regulated DNA methylation in hybrids.Our study indicated that both cis-and trans-regulated DNA methylation played roles in hybrids,when cis-regulation played a major role in CG methylation and trans-regulation played major roles in CHG and CHH methylation.In addition,we observed correlations between trans-regulated DNA methylation and siRNA densities.Enriched siRNA regions were significantly concurrent with highly trans-regulated DNA methylation regions.Our results illustrated DNA methylation regulation patterns integrated with siRNAs in Arabidopsis hybrids,and shed light on understanding the mechanism of epigenetic reprogramming for hybrid vigor.
