为筛选对黄瓜白粉病菌具有拮抗作用的生防菌株,从温室土壤样品中分离到细菌菌株692株。经室内平板拮抗性筛选和盆栽防治试验,筛选出菌株Bs-18对黄瓜白粉病具有良好的防治作用,盆栽防效达82.22%。对菌株Bs-18进行形态特征、生理生化特性...为筛选对黄瓜白粉病菌具有拮抗作用的生防菌株,从温室土壤样品中分离到细菌菌株692株。经室内平板拮抗性筛选和盆栽防治试验,筛选出菌株Bs-18对黄瓜白粉病具有良好的防治作用,盆栽防效达82.22%。对菌株Bs-18进行形态特征、生理生化特性和16S r DNA测序分析,将其鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。展开更多
[Objective] This study was conducted to screen resources for biological control of soil-borne diseases of tomato. [Method] Antagonistic bacteria against Ral- stonia solanacearum and Fusarium oxysporum were isolated an...[Objective] This study was conducted to screen resources for biological control of soil-borne diseases of tomato. [Method] Antagonistic bacteria against Ral- stonia solanacearum and Fusarium oxysporum were isolated and purified from soil samples by dilution plating and confronting incubation on PDA plates, then the antibacterial spectrum and metabolic secretions of the bacterial isolates were measured, and their species were identified by establishing phylogenetic trees with Neighbor-Joining method. [Result] From the 60 soil samples, 10 of 59 antagonistic bacteria isolates against R. solanacearum showed inhibition zone diameter 〉 25 mm, and 4 of 30 strains against F. oxysporum exhibited inhibition rate 〉 30%. The bacteriostatic substances and antibacterial spectrum analysis on above 14 isolates revealed that four strains N23-2, N58-2, NF59-3 and NF61-1 had good antago- nism against pathogenic bacteria and fungi; 12 strains produced cellulose, 11 strains produced proteases, and 6 strains produced siderophores. The molecular identification experiments indicated that four strains were members of Paenibacillus, three strains of Streptomyces, three strains of Pseudomonas strains, and four strains of Bacillus strains.展开更多
[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl...[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified enclophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [ Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [ Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.展开更多
文摘为筛选对黄瓜白粉病菌具有拮抗作用的生防菌株,从温室土壤样品中分离到细菌菌株692株。经室内平板拮抗性筛选和盆栽防治试验,筛选出菌株Bs-18对黄瓜白粉病具有良好的防治作用,盆栽防效达82.22%。对菌株Bs-18进行形态特征、生理生化特性和16S r DNA测序分析,将其鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。
文摘[Objective] This study was conducted to screen resources for biological control of soil-borne diseases of tomato. [Method] Antagonistic bacteria against Ral- stonia solanacearum and Fusarium oxysporum were isolated and purified from soil samples by dilution plating and confronting incubation on PDA plates, then the antibacterial spectrum and metabolic secretions of the bacterial isolates were measured, and their species were identified by establishing phylogenetic trees with Neighbor-Joining method. [Result] From the 60 soil samples, 10 of 59 antagonistic bacteria isolates against R. solanacearum showed inhibition zone diameter 〉 25 mm, and 4 of 30 strains against F. oxysporum exhibited inhibition rate 〉 30%. The bacteriostatic substances and antibacterial spectrum analysis on above 14 isolates revealed that four strains N23-2, N58-2, NF59-3 and NF61-1 had good antago- nism against pathogenic bacteria and fungi; 12 strains produced cellulose, 11 strains produced proteases, and 6 strains produced siderophores. The molecular identification experiments indicated that four strains were members of Paenibacillus, three strains of Streptomyces, three strains of Pseudomonas strains, and four strains of Bacillus strains.
文摘[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified enclophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [ Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [ Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.
