Endophytic bacteria B20-006,an antagonistic microbe,was applied to coat maize seeds and resulted in 67.9% control effect on maize sheath blight caused by Rhizoctonia solani.Moreover,B20-006 was ensured the ability to ...Endophytic bacteria B20-006,an antagonistic microbe,was applied to coat maize seeds and resulted in 67.9% control effect on maize sheath blight caused by Rhizoctonia solani.Moreover,B20-006 was ensured the ability to penetrate roots,and in turn move along vascular elements of maize plants.For further study of biocontrol mechanism of the antagonistic agent,the changes of major defensive enzymes(PAL,POD,SOD and EST) in specific activity were assayed in plants due to interaction between B20-006 and host maize.展开更多
文摘Endophytic bacteria B20-006,an antagonistic microbe,was applied to coat maize seeds and resulted in 67.9% control effect on maize sheath blight caused by Rhizoctonia solani.Moreover,B20-006 was ensured the ability to penetrate roots,and in turn move along vascular elements of maize plants.For further study of biocontrol mechanism of the antagonistic agent,the changes of major defensive enzymes(PAL,POD,SOD and EST) in specific activity were assayed in plants due to interaction between B20-006 and host maize.
文摘对前期筛选得到的猕猴桃溃疡病病原菌丁香假单胞菌亚种(Pseudomonas syringae pv.actinidiae,PSA)拮抗菌进行了鉴定和抗菌物质分离及分析。用革兰氏染色鉴别菌株;将硫酸铵沉淀溶液进行牛津杯抑菌试验,观察抑菌圈的大小;利用Tricine-SDS-PAGE电泳分析硫酸铵沉淀的抑菌物质;然后溶解沉淀进行去离子水透析脱盐,超滤浓缩后用葡聚糖凝胶柱进行分子筛凝胶过滤层析,最后采用高效液相色谱分析其分子量大小。结果发现:可判断有效抑制PSA生长的假单胞菌菌落形态特征为圆形菌落、乳白色、光滑、凸起、边缘整齐、不透明、不运动,为革兰氏阴性菌,菌体呈短杆状;其抑菌物质为细菌素,在40%和60%硫酸铵饱和度下提取效果最明显,抑菌作用最好,细菌素分子量大小为7 k Da左右。为研发细菌素产品提供了新思路,为防治猕猴桃溃疡病提供了理论基础和应用指导。
