Nanomaterials have been used increasingly in a wide variety of applications, and some of them have shown toxic effects on experimental animals and cells. In this study, a previously established photoelectrochemical DN...Nanomaterials have been used increasingly in a wide variety of applications, and some of them have shown toxic effects on experimental animals and cells. In this study, a previously established photoelectrochemical DNA sensor was employed to rapidly detect DNA damage induced by polystyrene nanosphere （PSNS） suspensions. In the sensor, a double-stranded DNA film was assembled on a semiconductor electrode, and a DNA intercalator, Ru（bpy）2（dppz）2＋ （bpy = 2,2＇-bipyridine, dppz = dipyrido[3,2-a：2＇,3＇-c]phenazine） was used as the photoelectrochemical signal indicator. After the DNA-modified electrode was exposed to 2.0 mg/mL PSNS suspension, photocurrent of DNA-bound Ru（bpy）2（dppz）2＋ decreased by about 20%. The decrease is attributed to the chemical damage of DNA and consequently less binding of Ru（bpy）2（dppz）2＋ molecules to the electrode. Gel electrophoresis of DNA samples incubated with PSNS suspension confirmed DNA damage after the chemical exposure. However, in both photoelectrochemical and gel electrophoresis experiments, extensively washed PSNS did not induce any DNA damage, and the supernatant of PSNS suspension exhibited comparable DNA damage as the unwashed PSNS suspension. Furthermore, UV-visible absorption spectrum of the supematant displayed a pattern very similar to that of styrene oxide （SO）, a compound which has been shown to induce DNA damage by forming covalent DNA adducts. It is therefore suggested that styrene oxide and other residual chemicals in the PSNS may be responsible for the observed DNA damage. The results highlight the importance of full characterization of nanomaterials before their toxicity study, and demonstrate the utility of photoelectrochemical DNA sensors in the rapid assessment of DNA damage induced by chemicals and nanomaterials.展开更多
Vibration behaviors of bogie hunting motion contain key information that dominates the dynamic performance of rail vehicles,in which the eigenvalue of each mode reflects the damping ratio and the natural frequency.Thi...Vibration behaviors of bogie hunting motion contain key information that dominates the dynamic performance of rail vehicles,in which the eigenvalue of each mode reflects the damping ratio and the natural frequency.This paper focuses on the root loci curves of bogie hunting motion,starting from a rigid bogie,then to a bogie with flexible primary suspension.With regard to the rigid bogie,analytical formulas for the eigenvalues,the critical speed as well as the corresponding hunting frequency are derived and verified.While for the flexible bogie,the root loci curves are calculated numerically.The study shows that both free rigid bogie and free wheelset are dynamically unstable at any speed.The critical speed increases with diminished wheel-rail conicity,track gauge,and wheelset and bogie inertia,and with increased wheelbase and wheel radius.The dominating factors such as the stiffness of the primary suspension and the wheel-rail conicity should be optimized for a practical design.The influences of the damping coefficients and the variations of creep coefficients are negligible.The motor suspension affects the root loci curves and the critical speed significantly.Both inappropriate motor suspension design and rigidly suspended motor reduce the critical speed.The increase of critical speed by a motor suspension can only be achieved when the lower natural frequency of the motor-bogie frame-wheelsets system coincides with or is close to the hunting frequency.Special care should be taken for the design of motor suspension,the first is to avoid the decreased damping ratio in a certain speed range below the critical speed and the second is that the variations of parameters should not induce the rapid reduction of the critical speed.The main feature of the present study is that the root loci curves,which are derived as analytical formulas or calculated numerically,are used to study the vibrational behaviors of bogie hunting motion.Both the influencing laws of the dominating parameters and the principles regarding the motor suspension are significant for the stability design of modem railway vehicles which may use innovative structures/materials as well as modem control and monitoring technologies.展开更多
基金supported by the National Basic Research Program of China (2011CB936001)the National Natural Science Foundation of China (20825519, 20890112 & 20921063)Beijing Municipal Education Committee (KZ201110005006)
文摘Nanomaterials have been used increasingly in a wide variety of applications, and some of them have shown toxic effects on experimental animals and cells. In this study, a previously established photoelectrochemical DNA sensor was employed to rapidly detect DNA damage induced by polystyrene nanosphere （PSNS） suspensions. In the sensor, a double-stranded DNA film was assembled on a semiconductor electrode, and a DNA intercalator, Ru（bpy）2（dppz）2＋ （bpy = 2,2＇-bipyridine, dppz = dipyrido[3,2-a：2＇,3＇-c]phenazine） was used as the photoelectrochemical signal indicator. After the DNA-modified electrode was exposed to 2.0 mg/mL PSNS suspension, photocurrent of DNA-bound Ru（bpy）2（dppz）2＋ decreased by about 20%. The decrease is attributed to the chemical damage of DNA and consequently less binding of Ru（bpy）2（dppz）2＋ molecules to the electrode. Gel electrophoresis of DNA samples incubated with PSNS suspension confirmed DNA damage after the chemical exposure. However, in both photoelectrochemical and gel electrophoresis experiments, extensively washed PSNS did not induce any DNA damage, and the supernatant of PSNS suspension exhibited comparable DNA damage as the unwashed PSNS suspension. Furthermore, UV-visible absorption spectrum of the supematant displayed a pattern very similar to that of styrene oxide （SO）, a compound which has been shown to induce DNA damage by forming covalent DNA adducts. It is therefore suggested that styrene oxide and other residual chemicals in the PSNS may be responsible for the observed DNA damage. The results highlight the importance of full characterization of nanomaterials before their toxicity study, and demonstrate the utility of photoelectrochemical DNA sensors in the rapid assessment of DNA damage induced by chemicals and nanomaterials.
基金supported by the National Natural Science Foundation of China(Grant Nos.51805452,and 51935002)the Independent Research Project of State Key Laboratory of Traction Power(Grant No.2020TPL-T02).
文摘Vibration behaviors of bogie hunting motion contain key information that dominates the dynamic performance of rail vehicles,in which the eigenvalue of each mode reflects the damping ratio and the natural frequency.This paper focuses on the root loci curves of bogie hunting motion,starting from a rigid bogie,then to a bogie with flexible primary suspension.With regard to the rigid bogie,analytical formulas for the eigenvalues,the critical speed as well as the corresponding hunting frequency are derived and verified.While for the flexible bogie,the root loci curves are calculated numerically.The study shows that both free rigid bogie and free wheelset are dynamically unstable at any speed.The critical speed increases with diminished wheel-rail conicity,track gauge,and wheelset and bogie inertia,and with increased wheelbase and wheel radius.The dominating factors such as the stiffness of the primary suspension and the wheel-rail conicity should be optimized for a practical design.The influences of the damping coefficients and the variations of creep coefficients are negligible.The motor suspension affects the root loci curves and the critical speed significantly.Both inappropriate motor suspension design and rigidly suspended motor reduce the critical speed.The increase of critical speed by a motor suspension can only be achieved when the lower natural frequency of the motor-bogie frame-wheelsets system coincides with or is close to the hunting frequency.Special care should be taken for the design of motor suspension,the first is to avoid the decreased damping ratio in a certain speed range below the critical speed and the second is that the variations of parameters should not induce the rapid reduction of the critical speed.The main feature of the present study is that the root loci curves,which are derived as analytical formulas or calculated numerically,are used to study the vibrational behaviors of bogie hunting motion.Both the influencing laws of the dominating parameters and the principles regarding the motor suspension are significant for the stability design of modem railway vehicles which may use innovative structures/materials as well as modem control and monitoring technologies.
