[Objective] The aim was to study the relationship of plant characters with bud living rate and mother stem emergence rate of ratoon rice before harvest. [Method] Eighteen mid-season hybrid rice cultivators approved re...[Objective] The aim was to study the relationship of plant characters with bud living rate and mother stem emergence rate of ratoon rice before harvest. [Method] Eighteen mid-season hybrid rice cultivators approved recently were taken as materials and relationship of plant characters including bud living rate and moth- er stem emergence rate of ratoon rice before harvest was explored, based on relat- ed data. [Result] The shorter rice with lower harvesting index is the main character of high emergence rate for first cropping rice; decline of plant height and increase of ear-bearing percentage would improve emergence of regenerative seedlings. [Conclusion] The research provided scientific reference for breeding of rice cultivars with high regenerative capacity.展开更多
AIM: To investigate the prognostic significance of phosphatase regenerating liver 3 (PRL-3) protein expression in gastric cancer.METHODS: PRL-3 expression in paraffin-embedded tumor specimens from 293 patients wit...AIM: To investigate the prognostic significance of phosphatase regenerating liver 3 (PRL-3) protein expression in gastric cancer.METHODS: PRL-3 expression in paraffin-embedded tumor specimens from 293 patients with gastric cancer was studied retrospectively by immunohistochemistry. Nonoclonal antibody specifically against PRL-3, 3B6, was obtained with hybridoma technique.RESULTS: Positive PRL-3 expression was detected in 43.3% (227 of 293) of gastric cancer cases. High expression of PRL-3 was positively correlated with tumor size, depth of invasion, vascular/lymphatic invasion, lymph node metastasis, high TNM stage and tumor recurrence. Patients with positive PRL-3 expression had a significantly lower 5-year survival rate than those with negative expression (28.3% vs 52.9%, P 〈 0.0001). Patients who received curative surgery, and with positive PRL-3 expression had a significant shorter overall survival and disease-free disadvantage over patients with negative expression (hazard ratio of 16.7 and 16.6, respectively; P 〈 0.0001 for both). Multivariate analysis revealed that PRL-3 expression was an independent prognostic indicator for overall and disease-free survival of gastric cancer patients, particularly for survival in TNM stage Ⅲ patients. CONCLUSION: PRL-3 expression is a new independent prognostic indicator to predict the potential of recurrence and survival in patients with gastric cancer at the time of tumor resection,展开更多
In the study, Southwest peony varieties group of Paeonia suffruticosa "Hongdan Lan" were analyzed, test was mainly divided into two parts, one is the nature of the low temperature, through the external environment i...In the study, Southwest peony varieties group of Paeonia suffruticosa "Hongdan Lan" were analyzed, test was mainly divided into two parts, one is the nature of the low temperature, through the external environment is mainly (temperature, light) gibberellin and nutrition solution regulation, on the Tianpeng peony florescence control technological research. The second is to explore the flower forcing peony after anthesis regeneration technique. The main research results: comprehensive gibberellin peony flower induction of morphological and physiological indexes, such as gibberellin concentration of 150 mgFL and 200 mg/L the effect of flower induction was better than the other treatments; gibberelin (GA3) breaking the dormancy of the peony to blossom ahead of time has the promoter action, also has certain effect on nutritional growth; to premature flowering the abscisic acid (ABA) has opposite effect; whereas auxin (IAA) and corn (ZT) effect was not obvious; rejuvenation results showed that after a year of conservation and managemental measures, flower induction after the flower of peony played a very important role in the restoration. After the winter flower induction, peony flowerpot of soil hardening, through the soil replacement, repair of roots, flower peony recovery showed a very significant effect.展开更多
OBJECTIVE To analyze the expression and levels of serum proinflammatory cytokines including tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6 in patients with hepatocellular carcinoma (HCC), who recei...OBJECTIVE To analyze the expression and levels of serum proinflammatory cytokines including tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6 in patients with hepatocellular carcinoma (HCC), who received orthotopic liver transplantation (OLT).METHODS The blood samples of 20 consecutive HCC patients who underwent liver transplantation were detected and analyzed for the clinical serum biochemical parameters, TNF-α and IL-6.Blood samples were drawn from the radial artery at planned time points: preoperatively, intraoperatively, and postoperatively.Levels of serum TNF-α and IL-6 were detected with enzymelinked immunosorbent assay (ELISA).RESULTS The levels of serum TNF-αand IL-6 increased significantly at reperfusion phase compared with those detected preoperatively (P 〈 0.01), and the level of serum IL-6 remained significantly higher until the third day after the liver transplantation. There was a significant correlation between TNF-αand IL-6 (P〈0.001).CONCLUSION This research into the effects of the proinflammatory cytokines on liver transplantation has provided new insights into the mechanisms of ischemia and reperfusion injury to OLT.展开更多
Objective:To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.Methods:Sixty male white Wistar rats were divided into four experimental groups randomly...Objective:To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.Methods:Sixty male white Wistar rats were divided into four experimental groups randomly (n=15).In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle.In treatment group the defect was bridged using a silicone graft filled with 10μL VEGF.In silicone group the graft was filled with phosphate-buffered saline.In sham-operated group the sciatic nerve was exposed and manipulated.Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4,8 and 12 weeks after operation.Results:Behavioral test,functional study of sciatic nerve,gastrocnemius muscle mass and morphometric indices confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P〈0.05).In immunohistochemical assessment,reactions to S-100 in VEGF group were more positive than that in silicone group.Conclusion:Local administration of VEGF will improve functional recovery and morphometric indices of sciatic nerve.展开更多
基金Supported by Sichuan Financial and Engineering Foundation for the Young(2007QNJJ-020)Sichuan Cultivation Foundation for Academy leaders+3 种基金Sichuan Key Project of Rice BreedingConstruction of Rice Industry System in SouthwestNational Crop-harvesting ProjectSichuan Financial Promotion~~
文摘[Objective] The aim was to study the relationship of plant characters with bud living rate and mother stem emergence rate of ratoon rice before harvest. [Method] Eighteen mid-season hybrid rice cultivators approved recently were taken as materials and relationship of plant characters including bud living rate and moth- er stem emergence rate of ratoon rice before harvest was explored, based on relat- ed data. [Result] The shorter rice with lower harvesting index is the main character of high emergence rate for first cropping rice; decline of plant height and increase of ear-bearing percentage would improve emergence of regenerative seedlings. [Conclusion] The research provided scientific reference for breeding of rice cultivars with high regenerative capacity.
基金Supported by Grant for Key Technology Research and Development Program 2002BA711A06National Basic Research Priorities Program 973 Project 1998051203the Ministry of Science and Technology of China,and grant H020920030390,from the Beijing Science and Technology Commission
文摘AIM: To investigate the prognostic significance of phosphatase regenerating liver 3 (PRL-3) protein expression in gastric cancer.METHODS: PRL-3 expression in paraffin-embedded tumor specimens from 293 patients with gastric cancer was studied retrospectively by immunohistochemistry. Nonoclonal antibody specifically against PRL-3, 3B6, was obtained with hybridoma technique.RESULTS: Positive PRL-3 expression was detected in 43.3% (227 of 293) of gastric cancer cases. High expression of PRL-3 was positively correlated with tumor size, depth of invasion, vascular/lymphatic invasion, lymph node metastasis, high TNM stage and tumor recurrence. Patients with positive PRL-3 expression had a significantly lower 5-year survival rate than those with negative expression (28.3% vs 52.9%, P 〈 0.0001). Patients who received curative surgery, and with positive PRL-3 expression had a significant shorter overall survival and disease-free disadvantage over patients with negative expression (hazard ratio of 16.7 and 16.6, respectively; P 〈 0.0001 for both). Multivariate analysis revealed that PRL-3 expression was an independent prognostic indicator for overall and disease-free survival of gastric cancer patients, particularly for survival in TNM stage Ⅲ patients. CONCLUSION: PRL-3 expression is a new independent prognostic indicator to predict the potential of recurrence and survival in patients with gastric cancer at the time of tumor resection,
文摘In the study, Southwest peony varieties group of Paeonia suffruticosa "Hongdan Lan" were analyzed, test was mainly divided into two parts, one is the nature of the low temperature, through the external environment is mainly (temperature, light) gibberellin and nutrition solution regulation, on the Tianpeng peony florescence control technological research. The second is to explore the flower forcing peony after anthesis regeneration technique. The main research results: comprehensive gibberellin peony flower induction of morphological and physiological indexes, such as gibberellin concentration of 150 mgFL and 200 mg/L the effect of flower induction was better than the other treatments; gibberelin (GA3) breaking the dormancy of the peony to blossom ahead of time has the promoter action, also has certain effect on nutritional growth; to premature flowering the abscisic acid (ABA) has opposite effect; whereas auxin (IAA) and corn (ZT) effect was not obvious; rejuvenation results showed that after a year of conservation and managemental measures, flower induction after the flower of peony played a very important role in the restoration. After the winter flower induction, peony flowerpot of soil hardening, through the soil replacement, repair of roots, flower peony recovery showed a very significant effect.
文摘OBJECTIVE To analyze the expression and levels of serum proinflammatory cytokines including tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6 in patients with hepatocellular carcinoma (HCC), who received orthotopic liver transplantation (OLT).METHODS The blood samples of 20 consecutive HCC patients who underwent liver transplantation were detected and analyzed for the clinical serum biochemical parameters, TNF-α and IL-6.Blood samples were drawn from the radial artery at planned time points: preoperatively, intraoperatively, and postoperatively.Levels of serum TNF-α and IL-6 were detected with enzymelinked immunosorbent assay (ELISA).RESULTS The levels of serum TNF-αand IL-6 increased significantly at reperfusion phase compared with those detected preoperatively (P 〈 0.01), and the level of serum IL-6 remained significantly higher until the third day after the liver transplantation. There was a significant correlation between TNF-αand IL-6 (P〈0.001).CONCLUSION This research into the effects of the proinflammatory cytokines on liver transplantation has provided new insights into the mechanisms of ischemia and reperfusion injury to OLT.
文摘Objective:To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.Methods:Sixty male white Wistar rats were divided into four experimental groups randomly (n=15).In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle.In treatment group the defect was bridged using a silicone graft filled with 10μL VEGF.In silicone group the graft was filled with phosphate-buffered saline.In sham-operated group the sciatic nerve was exposed and manipulated.Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4,8 and 12 weeks after operation.Results:Behavioral test,functional study of sciatic nerve,gastrocnemius muscle mass and morphometric indices confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P〈0.05).In immunohistochemical assessment,reactions to S-100 in VEGF group were more positive than that in silicone group.Conclusion:Local administration of VEGF will improve functional recovery and morphometric indices of sciatic nerve.