Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study...Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.展开更多
Objective To investigate the etiology and relative factors of dilated cardiomyopathy (DCM) in Chinese patients. Methods A case-control study was conducted to compare 233 patients with DCM in high-incidence areas (c...Objective To investigate the etiology and relative factors of dilated cardiomyopathy (DCM) in Chinese patients. Methods A case-control study was conducted to compare 233 patients with DCM in high-incidence areas (case group) and 150 patients with stable angina pectoris (control group). Life styles and history of diseases information was collected by questionaire; human anti-myocardial antibody lgG (AMA- IgG), human Coxsackie B virus IgG (CBV- IgG) and human adenovirus antibody IgG (ADV- IgG) were measured with ELISA. General chemical and toxicological indicators in drink water from high and low prevalence areas and serum trace elements also were compared. Results 1) Compared with the control group, the case group had more farmers (P 〈 0.01), with low average incomes (P 〈 0.01 ), higher alcohol consumption (P 〈 0.01) and higher incidence of the history of myocarditis (P 〈 0.01). 2) AMA-IgG, CBV-IgG and ADV-IgG levels were low and the positive rates ofAMA-IgG, CBV-IgG and ADV-IgG of patients with DCM were respectively 7.78%, 6.67% and 6.67%, no statistical significance comparing with those in the control group. 3) The content of iron (1.36±2.18 vs 0.39±0.67 rag/L, P〈0.05) and manganese (0.38±0.35 vs 0.15±0.14, P〈0.01) in drinking water of high-incidence areas was significantly higher than that in low-incidence areas. 4) The content of serum iron (69.1±57.8 vs 20.0±17.5 umol/L, P〈0.01 ) and copper (25.7±4.2 vs 19.7±4.5 umol/L, P〈0.01) in the case group evidently exceeded the normal range and obviously higher than that in the control group. Conclusions 1) The incidence of some DCM is related with low incomes, high alcohol consumption and myoearditis. 2) These data do not support that DCM is related with persistent virus infection and autoimmunization; 3) Iron and manganese contents exceeding standards in drinking water and the high content of serum iron and copper is comparatively related with the incidence of DCM.展开更多
AIM:To establish an appropriate primate model of fulminant hepatic failure (FHF).METHODS:We have,for the first time,established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and...AIM:To establish an appropriate primate model of fulminant hepatic failure (FHF).METHODS:We have,for the first time,established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin.Clinical features,biochemical indexes,histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model.RESULTS:Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration.Coagulation activity was significantly decreased.Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h.The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h,respectively.The autopsy showed typical yellow atrophy of the liver.Hepatic encephalopathy of the models was also confirmed by hepatic coma,MRI and pathological changes of cerebral edema.The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices,imaging and histopathology.CONCLUSION:We have established an appropriate large primate model of FHF,which is closely similar to clinic cases,and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.展开更多
Analytic hierarchy process(Group AHP) is combined with two different methods of assigning experts' priority to weight indicators in building energy efficiency assessment.One is to assign the experts' priority ...Analytic hierarchy process(Group AHP) is combined with two different methods of assigning experts' priority to weight indicators in building energy efficiency assessment.One is to assign the experts' priority averagely,and the other is to use cluster analysis to assign experts' priority.The results show that,1) Different expert's priority assigns result in great different weights of indicators in building energy efficiency assessment,therefore,the method of assigning experts' priority should be taken into account carefully while weighting indicators of building energy efficiency assessment using Group AHP;2) Three indicators are found to be overwhelmingly important in residential building energy efficiency assessment in the hot summer and cold winter zone in China.They are 'Outdoor & indoor shadow','Heating & air-conditioning facilities' and 'Insulation of envelope';3) The method combining cluster analysis with Group AHP to weight indicator of building energy efficiency assessment has the advantage of finding overwhelming important indicator,whereas,some less important indicators have a tendency to be ignored.A useful reference is provided for building energy conservation including policy revision and energy efficient residential building design.展开更多
AIM:To assess the hepatic changes after induction of different periods of renal ischemia. METHODS:Rats were subjected to either sham operation or ischemia (30,45 and 60 min) followed by 60 min reperfusion. Liver and r...AIM:To assess the hepatic changes after induction of different periods of renal ischemia. METHODS:Rats were subjected to either sham operation or ischemia (30,45 and 60 min) followed by 60 min reperfusion. Liver and renal functional indices were measured. Hepatic glutathione (GSH) and ferric reducing antioxidant power levels and the concentration of interleukin (IL)-10 and tumor necrosis factor (TNF-α) were evaluated. Portions of liver and kidney tissues were fixed for histological evaluation. RESULTS:Forty-five minutes renal ischemia followed by 60 min reperfusion caused significant changes in liver structure and a significant reduction in renalfunction. These rats showed a significant decrease in liver GSH,as well as a significant increase in TNF-α and IL-10 concentrations. These results demonstrated that renal ischemia caused changes in liver histology,function,oxidative stress and inflammatory status,which led to a reduction in hepatic antioxidant capacity. With 30 min ischemia,the magnitude of these changes was less than those with 45 or 60 min ischemia.CONCLUSION:A minimum of 45 min ischemia is needed to study the effects of renal injury on the liver as a remote organ.展开更多
The deformation performance index limits of high reinforced concrete (RC) shear wall components based on Chinese codes were discussed by the nonlinear finite element method. Two typical RC shear wall specimens in th...The deformation performance index limits of high reinforced concrete (RC) shear wall components based on Chinese codes were discussed by the nonlinear finite element method. Two typical RC shear wall specimens in the previous work were first used to verify the correctness of the nonlinear finite element method. Then, the nonlinear finite element method was applied to study the deformability of a set of high RC shear wall components designed according to current Chinese codes and with shear span ratio λ≥2.0. Parametric studies were made on the influence of shear span ratio, axial compression ratio, ratio of flexural capacity to shear capacity and main flexural reinforcement ratio of confined botmdary members. Finally, the deformation performance index and its limits of high RC shear wall components under severe earthquakes were proposed by the finite element model results, which offers a reference in determining the performance status of RC shear wall components designed based on Chinese codes.展开更多
There are essentially six sources for obsidians in the Mediterranean Basin: Mount Arci in Sardinia, the islands of Lipari and Pantelleria in Sicily and Palmarola in Latium, as well as the islands of Melos and Gyali i...There are essentially six sources for obsidians in the Mediterranean Basin: Mount Arci in Sardinia, the islands of Lipari and Pantelleria in Sicily and Palmarola in Latium, as well as the islands of Melos and Gyali in the south-eastern part of Greece. Identification and quantification of principal and trace elements which compose this volcanic glass allow source tracking. The present study proposes a investigation methodology, which was developed on a sample set composed by archaeological obsidian findings from Neolithic sites in Latium; three different analytical techniques are compared: SEM-EDXS, ED-XRF and LA-ICP-MS, in order to gather a wide data set to be compared with data in literature and to be processed by Principal Component Analysis (PCA) for assigning a provenance to each find.展开更多
The concentration of Fe(II) is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe(II) is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maxi...The concentration of Fe(II) is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe(II) is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maximus, exposed to Fe(II) of different concentrations (0.01, 0.05, 0.1, 0.5, 1, and 2 mg/L) for 1, 7, 14, and 28 d, under the same ambient conditions of other parameters. Changes in respiratory rate, hematological parameters, and gill structure were determined. The results show that waterborne Fe(II) did not cause severe hematological perturbation to turbot. A low-medium Fe(II) concentration (lower than 0.1 mg/L) could boost the respiratory rate, and caused no or very limited damage to fish. A high Fe(II) concentration (0.1 mg/L or higher), however, caused gill damage, such as vacuoles in branchial lamellae, epithelial necrosis, and hypertrophy of epithelial cells, and even death after extended exposure time. Therefore, excess waterborne Fe(II) and long-term exposure to Fe(II) could be responsible for poor growth and high mortality of turbot in culture. The concentration of waterborne Fe(II) in turbot culture should be kept below 0.1 mg/L.展开更多
Here we present six key parameters that have been applied to evaluate the Silurian Longmaxi shale gas accumulations of southern Sichuan Province.These parameters include richness of organic matter,single layer shale t...Here we present six key parameters that have been applied to evaluate the Silurian Longmaxi shale gas accumulations of southern Sichuan Province.These parameters include richness of organic matter,single layer shale thickness,burial depth of the shale,maturity,shale properties,and brittle mineral content.In combination with drilling data,the lower limits of these six parameters for marine shale gas have been evaluated and the following conclusions are drawn:(1)Organic carbon recovery, gas content inversion,and production tests have been applied for the first time to measure the lower limit of TOC,which is determined to be 1.0%.(2)Based on the hydrocarbon generation and expulsion theory for source rocks and the characteristics of Longmaxi shale,the lower limit of the single-layer thickness is estimated to be 30 m,of which a thickness of at least 15 m should contain high-quality shale.(3)Based on the correlation of TOC and porosity,the lower limit of gas-filled porosity is 1.2%.(4)Other parameters,such as maturity,burial depth,and brittle mineral content have also been studied with regard to the geologic conditions of southern Sichuan.Based on the study results,an evaluation index system has been established for highly evolved marine shale gas,and the system has been applied successfully to geologically screened shale gas occurrences in southern Sichuan Province.展开更多
基金Supported by grants from the Science and Technology Development Project of Yantai(No.2008162)Natural Sciences Foundation of Shandong(No.ZR2013HL004)Science and Technology Important Project of Binzhou Medical College(No.BY20bkjzd04)
文摘Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.
文摘Objective To investigate the etiology and relative factors of dilated cardiomyopathy (DCM) in Chinese patients. Methods A case-control study was conducted to compare 233 patients with DCM in high-incidence areas (case group) and 150 patients with stable angina pectoris (control group). Life styles and history of diseases information was collected by questionaire; human anti-myocardial antibody lgG (AMA- IgG), human Coxsackie B virus IgG (CBV- IgG) and human adenovirus antibody IgG (ADV- IgG) were measured with ELISA. General chemical and toxicological indicators in drink water from high and low prevalence areas and serum trace elements also were compared. Results 1) Compared with the control group, the case group had more farmers (P 〈 0.01), with low average incomes (P 〈 0.01 ), higher alcohol consumption (P 〈 0.01) and higher incidence of the history of myocarditis (P 〈 0.01). 2) AMA-IgG, CBV-IgG and ADV-IgG levels were low and the positive rates ofAMA-IgG, CBV-IgG and ADV-IgG of patients with DCM were respectively 7.78%, 6.67% and 6.67%, no statistical significance comparing with those in the control group. 3) The content of iron (1.36±2.18 vs 0.39±0.67 rag/L, P〈0.05) and manganese (0.38±0.35 vs 0.15±0.14, P〈0.01) in drinking water of high-incidence areas was significantly higher than that in low-incidence areas. 4) The content of serum iron (69.1±57.8 vs 20.0±17.5 umol/L, P〈0.01 ) and copper (25.7±4.2 vs 19.7±4.5 umol/L, P〈0.01) in the case group evidently exceeded the normal range and obviously higher than that in the control group. Conclusions 1) The incidence of some DCM is related with low incomes, high alcohol consumption and myoearditis. 2) These data do not support that DCM is related with persistent virus infection and autoimmunization; 3) Iron and manganese contents exceeding standards in drinking water and the high content of serum iron and copper is comparatively related with the incidence of DCM.
基金Supported by National Basic Research Program of China,No.2009CB522401grand from Natural Science Foundation ofChina,No. 30870983 and 30971118
文摘AIM:To establish an appropriate primate model of fulminant hepatic failure (FHF).METHODS:We have,for the first time,established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin.Clinical features,biochemical indexes,histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model.RESULTS:Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration.Coagulation activity was significantly decreased.Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h.The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h,respectively.The autopsy showed typical yellow atrophy of the liver.Hepatic encephalopathy of the models was also confirmed by hepatic coma,MRI and pathological changes of cerebral edema.The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices,imaging and histopathology.CONCLUSION:We have established an appropriate large primate model of FHF,which is closely similar to clinic cases,and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.
基金Project(2010R10036) supported by the Science and Technology Department of Zhejiang Province, China
文摘Analytic hierarchy process(Group AHP) is combined with two different methods of assigning experts' priority to weight indicators in building energy efficiency assessment.One is to assign the experts' priority averagely,and the other is to use cluster analysis to assign experts' priority.The results show that,1) Different expert's priority assigns result in great different weights of indicators in building energy efficiency assessment,therefore,the method of assigning experts' priority should be taken into account carefully while weighting indicators of building energy efficiency assessment using Group AHP;2) Three indicators are found to be overwhelmingly important in residential building energy efficiency assessment in the hot summer and cold winter zone in China.They are 'Outdoor & indoor shadow','Heating & air-conditioning facilities' and 'Insulation of envelope';3) The method combining cluster analysis with Group AHP to weight indicator of building energy efficiency assessment has the advantage of finding overwhelming important indicator,whereas,some less important indicators have a tendency to be ignored.A useful reference is provided for building energy conservation including policy revision and energy efficient residential building design.
基金Supported by A grant from Tehran Medical Sciences University
文摘AIM:To assess the hepatic changes after induction of different periods of renal ischemia. METHODS:Rats were subjected to either sham operation or ischemia (30,45 and 60 min) followed by 60 min reperfusion. Liver and renal functional indices were measured. Hepatic glutathione (GSH) and ferric reducing antioxidant power levels and the concentration of interleukin (IL)-10 and tumor necrosis factor (TNF-α) were evaluated. Portions of liver and kidney tissues were fixed for histological evaluation. RESULTS:Forty-five minutes renal ischemia followed by 60 min reperfusion caused significant changes in liver structure and a significant reduction in renalfunction. These rats showed a significant decrease in liver GSH,as well as a significant increase in TNF-α and IL-10 concentrations. These results demonstrated that renal ischemia caused changes in liver histology,function,oxidative stress and inflammatory status,which led to a reduction in hepatic antioxidant capacity. With 30 min ischemia,the magnitude of these changes was less than those with 45 or 60 min ischemia.CONCLUSION:A minimum of 45 min ischemia is needed to study the effects of renal injury on the liver as a remote organ.
基金Project(2009ZA04) supported by the Independent Research Foundation of State Key Laboratory of Subtropical Architecture Science,China
文摘The deformation performance index limits of high reinforced concrete (RC) shear wall components based on Chinese codes were discussed by the nonlinear finite element method. Two typical RC shear wall specimens in the previous work were first used to verify the correctness of the nonlinear finite element method. Then, the nonlinear finite element method was applied to study the deformability of a set of high RC shear wall components designed according to current Chinese codes and with shear span ratio λ≥2.0. Parametric studies were made on the influence of shear span ratio, axial compression ratio, ratio of flexural capacity to shear capacity and main flexural reinforcement ratio of confined botmdary members. Finally, the deformation performance index and its limits of high RC shear wall components under severe earthquakes were proposed by the finite element model results, which offers a reference in determining the performance status of RC shear wall components designed based on Chinese codes.
文摘There are essentially six sources for obsidians in the Mediterranean Basin: Mount Arci in Sardinia, the islands of Lipari and Pantelleria in Sicily and Palmarola in Latium, as well as the islands of Melos and Gyali in the south-eastern part of Greece. Identification and quantification of principal and trace elements which compose this volcanic glass allow source tracking. The present study proposes a investigation methodology, which was developed on a sample set composed by archaeological obsidian findings from Neolithic sites in Latium; three different analytical techniques are compared: SEM-EDXS, ED-XRF and LA-ICP-MS, in order to gather a wide data set to be compared with data in literature and to be processed by Principal Component Analysis (PCA) for assigning a provenance to each find.
基金Supported by the National Key Technology R&D Program(Nos.2011BAD13B04,2006BAD09A11)Shandong Agricultural Seed Stock Breeding Project
文摘The concentration of Fe(II) is high in some groundwater supplies used in turbot culture, and the toxicity of waterborne Fe(II) is unknown. We investigated the stress responses of juvenile turbot, Scophthalmus maximus, exposed to Fe(II) of different concentrations (0.01, 0.05, 0.1, 0.5, 1, and 2 mg/L) for 1, 7, 14, and 28 d, under the same ambient conditions of other parameters. Changes in respiratory rate, hematological parameters, and gill structure were determined. The results show that waterborne Fe(II) did not cause severe hematological perturbation to turbot. A low-medium Fe(II) concentration (lower than 0.1 mg/L) could boost the respiratory rate, and caused no or very limited damage to fish. A high Fe(II) concentration (0.1 mg/L or higher), however, caused gill damage, such as vacuoles in branchial lamellae, epithelial necrosis, and hypertrophy of epithelial cells, and even death after extended exposure time. Therefore, excess waterborne Fe(II) and long-term exposure to Fe(II) could be responsible for poor growth and high mortality of turbot in culture. The concentration of waterborne Fe(II) in turbot culture should be kept below 0.1 mg/L.
基金supported by National Science Foundation for Distinguished Young Scholars of China(Grant No.51125019)
文摘Here we present six key parameters that have been applied to evaluate the Silurian Longmaxi shale gas accumulations of southern Sichuan Province.These parameters include richness of organic matter,single layer shale thickness,burial depth of the shale,maturity,shale properties,and brittle mineral content.In combination with drilling data,the lower limits of these six parameters for marine shale gas have been evaluated and the following conclusions are drawn:(1)Organic carbon recovery, gas content inversion,and production tests have been applied for the first time to measure the lower limit of TOC,which is determined to be 1.0%.(2)Based on the hydrocarbon generation and expulsion theory for source rocks and the characteristics of Longmaxi shale,the lower limit of the single-layer thickness is estimated to be 30 m,of which a thickness of at least 15 m should contain high-quality shale.(3)Based on the correlation of TOC and porosity,the lower limit of gas-filled porosity is 1.2%.(4)Other parameters,such as maturity,burial depth,and brittle mineral content have also been studied with regard to the geologic conditions of southern Sichuan.Based on the study results,an evaluation index system has been established for highly evolved marine shale gas,and the system has been applied successfully to geologically screened shale gas occurrences in southern Sichuan Province.