基于目标管理的指标责任图谱构建及应用

本文通过建立指标责任图谱,帮助指标相关责任人真正了解自身工作完成的实际水平,发现工作中的薄弱环节,制订切实可行的提升措施,提高其业务水平。通过制定责任指标目标,明确各部门职责及目标,实现以目标为导向的对标逐级评价,保证对标工作能够可控、可监督、可评价和能改进的目的,使基层一线员工真正地有目标、有范围、有指导地完成日常工作,提高工作效率,切实减轻工作负担。

均匀设计试验法在溢流阀锥阀芯优化中的应用

针对溢流阀在线检测系统中,锥阀芯材料为紫铜时,阀芯力学性能不满足设计要求的问题,提出通过优化部分机械结构参数,进而提升阀芯力学性能的研究方法。设计了一种基于均匀设计试验法的阀芯力学性能的仿真试验方案,借助于有限元法、均匀设计试验、动态响应模型等研究方法,分析了各参数对结构优化影响的敏感度,求解了多指标函数与结构参数之间的动态响应模型。最后,利用指标图谱和遗传算法对结构参数进行了优化计算,对比了均匀设计试验法和指标图谱法的优化结果。结果表明,均匀设计试验和指标图谱优化结果十分接近,均匀设计试验法适用于阀芯力学性能的优化设计。

Genetic Diversity Analysis and Fingerprint Construction of Major Mango Cultivars in China

[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding of mango. [Method] With 115 pairs of SSR primers, genetic diversity analysis and cluster analysis were performed for 30 mango cultivars, among which the genetic relationships were analyzed. [Result] Total 64 pairs of polymorphic primers were screened out from the 115 pairs of primers, and total 343 bands were amplified from the 30 cultivars with 73.2% of polymorphic bands. On average, 3.9 allelic loci were detected for each pair of primers with genetic diversity index of 0.5, Shannon＇s diversity index of 1.00 and polymorphism information content of 0.49, indicating higher genetic diversity. The cluster analysis showed that the 30 major cultivars could be classified into four categories. The first category included 14 cultivars; the second category included 11 cultivars, most of which were introduced from abroad; the third category included 4 cultivars, Le., Miansan, Parayinda, Baiyu and Hongxiangya： the fourth category included only one cultivar Maqiesu.By using 7 pairs of SSR markers, i.e., M42, M49, M54, M55, M96, M99 and M103, digital fingerprints were constructed for the 30 mango cultivars. [Conclusion] The 30 mango cultivars present more complex genomic genetics and abundant genetic information, and they have higher genetic diversity.

基于图谱指标的滚动轴承故障特征提取方法

为了更准确地提取滚动轴承振动信号的非线性故障特征,将图信号处理(GSP)引入机械故障诊断领域,提出了基于图谱指标的滚动轴承故障特征提取方法。该方法将滚动轴承的振动信号转化为路图信号后,提取多个图谱指标;用Fisher得分(FS)算法对图谱指标的敏感度进行排序,并选取若干个最敏感的图谱指标作为滚动轴承的故障特征参数;用K-均值聚类算法识别滚动轴承的不同故障。应用实例表明:当分别选取1~5个最优的图谱指标、时域指标和频域指标对不同轴承故障进行识别时,图谱指标均没有出现错误,而时域指标和频域指标都出现了不同数量的错误,因此,图谱指标对轴承故障的区分能力优于时域指标和频域指标。

Molecular Identification and Cultivar Fingerprints of Prunus persica (L.) Batsch Germplasms

[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.

An improved calibrated normalized resolution product(r*') and the application in HPLC fingerprints of soy isoflavone extract

In this paper, we proposed a novel resolution criterion（improved calibrated normalized resolution product, r＊＇） to evaluate separation quality of fingerprints. By comparing with the calibrated normalized resolution product（r＊） and the hierarchical chromatographic response function（HCRF）, the validity of this criterion was demonstrated by experimental chromatograms. The soy isoflavone extract was selected as the analytical object. The initial and end percentages of methanol and elution time affecting gradient elution were tested by orthogonal design. The final optimized conditions were as follows. It was detected by UV absorbance at 254 nm, column temperature was maintained at 36 oC, solvent A was 0.1%（v/v） acetic acid, solvent B was methanol, gradient elution was from 34% to 65% B in a linear gradient in 25 min, and the flow-rate was set at 1.0 m L/min. In addition, the main ingredients of the soy isoflavone extract were confirmed by LC-ESI/MS.

Principle of nonlinear chemical fingerprint by using dissipative components in samples as well as calculation and evaluation of similarity

Under the conditions of constant temperature and pressure,different influences of samples with different chemical components on the mechanism of nonlinear chemical reaction will cause different changes of the potential-time relationship curve of the nonlinear chemical reaction system.Using it as the character,and using the B-Z nonlinear chemical system to use acetone and substrates in samples as main dissipative substances qua an example,the principle of nonlinear chemical fingerprint has been researched and discussed in detail.At the same time,the general method for calculating the system similarity about nonlinear chemical fingerprint was also put forward,and similarities of nonlinear chemistry fingerprints of different batches of Guhan Yangshengjing and 18 sorts of other samples were calculated by Euclidean distance,correlation coefficient,included angle cosine and system similarity,at the same time,the various similarities were analyzed.The results showed that,both of correlation coefficient and included angle cosine are unable to be used as the criterion for quantitatively evaluating the similarity of nonlinear chemistry fingerprint;as non-parametric similarity,Euclidean distance can accurately reflect the feature differences in the fingerprints,but as parametric similarity,sometimes,Euclidean distance can not accurately reflect the relative extent of characteristic difference in the nonlinear chemical fingerprints;system similarity can most truthfully reflect the characteristic difference in the nonlinear chemical fingerprints,and is the best evaluating method among the four ones.Therefore,system similarity can be used to quantitatively calculate the similar extent between the nonlinear chemical fingerprints.An economical,simple and convenient,easy pushing and effective method for identifying and evaluating complicated samples has successfully been put forward.