目的采用双定性双定量相似度作为评价指标,建立了复方丹参滴丸(CDDP)中低波长紫外吸收指纹成分的控制方法。方法采用RP-HPLC法以Century SIL C18BDS柱(200 mm×4.6 mm,5μm);以色谱指纹图谱指数F为目标函数优化选择指纹图谱检测条件...目的采用双定性双定量相似度作为评价指标,建立了复方丹参滴丸(CDDP)中低波长紫外吸收指纹成分的控制方法。方法采用RP-HPLC法以Century SIL C18BDS柱(200 mm×4.6 mm,5μm);以色谱指纹图谱指数F为目标函数优化选择指纹图谱检测条件,确定流动相为水-乙腈低压梯度洗脱,紫外检测波长:203 nm,柱温:(30.00±0.15)℃,进样量:10μL。以双定性双定量相似度法对10批CDDP进行质量评价。结果以人参皂苷Rg1为参照物峰,确定18个共有指纹峰,建立了CDDP低波长紫外吸收指纹成分的HPLC指纹图谱。评价出10批CDDP的双定性双定量相似度均合格,表明此10批样品的低波长紫外吸收指纹成分的数量、分布比例和含量特征是十分相似的,表现出很好的质量均一性。结论本试验证明双定性双定量相似度法可有效控制CDDP中低波长紫外吸收指纹成分。展开更多
By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish ...By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish element fingerprints, and find out the characteristic elements. ICP-MS was used to measure the content of inorganic elements and map the element fingerprints. Additionally, WM/T2-2004 was applied to evaluate the quality of heavy metal elements, and the characteristic elements were determined by principal component analysis. The results showed that the contents of inorganic elements in Radix Pseudostellariae were between 0.057 and 959 mg/kg with the coefficients of variation ranging from 0.134 to 1.478, and the contents of Cd, As, Pb, and Hg were below the Standard of WM/T2-2004 in 90% of Radix Pseudostellariae. The standard limits of heavy metals in Radix Pseu- dostellariae were Cr≤6.5 mg/kg, Cu≤10 mg/kg, As≤2.0 mg /kg, Cd≤0.3 mg/kg, Hg≤0.15 mg/kg, and Pb≤4.0 mg/kg. The features of the inorganic eJements finger- prints could provide theoretical basis of identifying the quality of Radix Pseudostel- lariae and distinguishing Radix Pseudostellariae from other Chinese herbal medicines. The characteristic inorganic elements of Radix Pseudostellariae were found to be Cd, Cu, Co, Zn, Fe, Ca, Mg, and AI. Radix Pseudostellariae from Guizhou Province contained abundant inorganic elements, and the contents of heavy metals were below the evaluation criterion. The study provided a reference for the future development of the limiting values of heaw metals in Radix Pseudostellariae.展开更多
The increase of import/export of every kind of herbal products calls urgently for adequate controls. Analysis of herbal food supplement （botanicals） is a difficult task, like in the composition determination of a mu...The increase of import/export of every kind of herbal products calls urgently for adequate controls. Analysis of herbal food supplement （botanicals） is a difficult task, like in the composition determination of a multi-ingredient product, where several botanical drugs were used. Actually, this is an important argument in consideration of health security. The authors reported the results of an analytical approach based on HPTLC （high performance thin layer chromatography） fingerprints comparison and tailored to determine the composition of marketed multi-ingredient botanicals. The method gave positive data in case of the presence of 3-5 species, whereas difficulties were recorded when the number of plants is increased.展开更多
中药生物药剂学分类系统(biopharmaceutics classification system of Chinese materia medica,CMMBCS)构建的复杂层面为中药复方研究,在坚持多成分同时测定的前提下,开展渗透性研究时,其首要任务是明确透过肠壁而被吸收的成分来源,即...中药生物药剂学分类系统(biopharmaceutics classification system of Chinese materia medica,CMMBCS)构建的复杂层面为中药复方研究,在坚持多成分同时测定的前提下,开展渗透性研究时,其首要任务是明确透过肠壁而被吸收的成分来源,即具体归属于中药复方中的哪味药材。该研究利用化学成分指纹图谱技术(chemical fingerprint),采用离体翻转肠囊实验技术对经典名方葛根芩连方中的肠渗透多成分进行直观来源归属,为下一步的肠渗透性定性及定量研究奠定基础。展开更多
基金Supported by the Science and Technology Project of Zunyi City (Zunyi Science Cooperation[2016]12)Key Lab Construction Project of the Educational Department of Guizhou Province (Project No.:Guizhou Education Cooperation KY[2014]212)the Key Special Project for the Modernization of Chinese Traditional Medicine of Guizhou Province (Guizhou Science Cooperation[2012]6010)~~
文摘By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish element fingerprints, and find out the characteristic elements. ICP-MS was used to measure the content of inorganic elements and map the element fingerprints. Additionally, WM/T2-2004 was applied to evaluate the quality of heavy metal elements, and the characteristic elements were determined by principal component analysis. The results showed that the contents of inorganic elements in Radix Pseudostellariae were between 0.057 and 959 mg/kg with the coefficients of variation ranging from 0.134 to 1.478, and the contents of Cd, As, Pb, and Hg were below the Standard of WM/T2-2004 in 90% of Radix Pseudostellariae. The standard limits of heavy metals in Radix Pseu- dostellariae were Cr≤6.5 mg/kg, Cu≤10 mg/kg, As≤2.0 mg /kg, Cd≤0.3 mg/kg, Hg≤0.15 mg/kg, and Pb≤4.0 mg/kg. The features of the inorganic eJements finger- prints could provide theoretical basis of identifying the quality of Radix Pseudostel- lariae and distinguishing Radix Pseudostellariae from other Chinese herbal medicines. The characteristic inorganic elements of Radix Pseudostellariae were found to be Cd, Cu, Co, Zn, Fe, Ca, Mg, and AI. Radix Pseudostellariae from Guizhou Province contained abundant inorganic elements, and the contents of heavy metals were below the evaluation criterion. The study provided a reference for the future development of the limiting values of heaw metals in Radix Pseudostellariae.
文摘The increase of import/export of every kind of herbal products calls urgently for adequate controls. Analysis of herbal food supplement （botanicals） is a difficult task, like in the composition determination of a multi-ingredient product, where several botanical drugs were used. Actually, this is an important argument in consideration of health security. The authors reported the results of an analytical approach based on HPTLC （high performance thin layer chromatography） fingerprints comparison and tailored to determine the composition of marketed multi-ingredient botanicals. The method gave positive data in case of the presence of 3-5 species, whereas difficulties were recorded when the number of plants is increased.
文摘中药生物药剂学分类系统(biopharmaceutics classification system of Chinese materia medica,CMMBCS)构建的复杂层面为中药复方研究,在坚持多成分同时测定的前提下,开展渗透性研究时,其首要任务是明确透过肠壁而被吸收的成分来源,即具体归属于中药复方中的哪味药材。该研究利用化学成分指纹图谱技术(chemical fingerprint),采用离体翻转肠囊实验技术对经典名方葛根芩连方中的肠渗透多成分进行直观来源归属,为下一步的肠渗透性定性及定量研究奠定基础。
