To quickly find documents with high similarity in existing documentation sets, fingerprint group merging retrieval algorithm is proposed to address both sides of the problem:a given similarity threshold could not be t...To quickly find documents with high similarity in existing documentation sets, fingerprint group merging retrieval algorithm is proposed to address both sides of the problem:a given similarity threshold could not be too low and fewer fingerprints could lead to low accuracy. It can be proved that the efficiency of similarity retrieval is improved by fingerprint group merging retrieval algorithm with lower similarity threshold. Experiments with the lower similarity threshold r=0.7 and high fingerprint bits k=400 demonstrate that the CPU time-consuming cost decreases from 1 921 s to 273 s. Theoretical analysis and experimental results verify the effectiveness of this method.展开更多
To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the d...To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the differential expression proteins. Methods： Forty Kunming mice were randomly divided into infection group （20） injected with HCMVAD169 and control group （20） injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis（2-DE）, analyzed by Image Master 2D software, and identified by peptide mass fingerprint（PMF） and database searching, and make Western blotting analyses the differential expression of individual proteins. Results： Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry（MS） were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion： These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine.展开更多
基金Project(60873081) supported by the National Natural Science Foundation of ChinaProject(NCET-10-0787) supported by the Program for New Century Excellent Talents in University, ChinaProject(11JJ1012) supported by the Natural Science Foundation of Hunan Province, China
文摘To quickly find documents with high similarity in existing documentation sets, fingerprint group merging retrieval algorithm is proposed to address both sides of the problem:a given similarity threshold could not be too low and fewer fingerprints could lead to low accuracy. It can be proved that the efficiency of similarity retrieval is improved by fingerprint group merging retrieval algorithm with lower similarity threshold. Experiments with the lower similarity threshold r=0.7 and high fingerprint bits k=400 demonstrate that the CPU time-consuming cost decreases from 1 921 s to 273 s. Theoretical analysis and experimental results verify the effectiveness of this method.
基金Supported by the Program of Science and Technology from Shenzhen City, Guangdong Province (200802051)
文摘To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the differential expression proteins. Methods： Forty Kunming mice were randomly divided into infection group （20） injected with HCMVAD169 and control group （20） injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis（2-DE）, analyzed by Image Master 2D software, and identified by peptide mass fingerprint（PMF） and database searching, and make Western blotting analyses the differential expression of individual proteins. Results： Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry（MS） were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion： These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine.
