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浅谈中药材贮存保管
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作者 黄秋 《海峡药学》 1999年第S1期134-135,共2页
关键词 药材 药材质量 贮存保管 指药材 挥发油 变色的药材 温湿度影响 防治方法 根茎药材 药材保管
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A Molecular Approach to Identification of the Chinese Drug“pu Gong Ying”(Herba Taraxaci)and Six Adulterants byDNA Fingerprinting Using Random Primed PolymeraseChain Resaction(PCR) 被引量:3
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作者 曹晖 毕培曦 邵鹏柱 《Journal of Chinese Pharmaceutical Sciences》 CAS 1996年第4期186-194,共9页
DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbit... DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs. 展开更多
关键词 Chinese drug identification Taraxacum mongolicum DNA fingerprinting Polymerase chain reaction
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Fingerprint analysis of different Panax herbal species by HPLC-UV method 被引量:4
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作者 曾凤 王晓明 +2 位作者 杨敏 卢志强 果德安 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第4期277-281,共5页
Aim To establish a method for differentiating commercial samples of Panax species including notoginseng, cultivated ginseng (Chinese ginseng and Korean ginseng), wild ginseng, red ginseng, three types of American gi... Aim To establish a method for differentiating commercial samples of Panax species including notoginseng, cultivated ginseng (Chinese ginseng and Korean ginseng), wild ginseng, red ginseng, three types of American ginsengs, and one American ginseng preparation with their HPLC fingerprints for assnrning the quality of different commercial samples of Panax species. Methods HPLC-UV method was used to establish their fingerprints, Zorbax Extend C18 (250 mm×4.6 mm, 5 μm) was used as the analytical column, and acetonitrile/KH2PO4 aqueous solution was used as the mobile phase with gradient elution. Results The fingerprints of different commercial samples of Panax species varied in their holistic chromatograms and some specific constituents. Conclusion This method is reliable, reproducible and simple, It could be applied in the routine authentication of different commercial samples of Panax species 展开更多
关键词 GINSENG Panax species HPLC fingerprint
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