Objective To assess the role of p38 MAPK in protective effect of remifentanil preconditioning(RPC) on myocardial ischemia reperfusion injury in rat hearts.Methods Male Spargue-Dawley rats weighing 300 g to 350 g were ...Objective To assess the role of p38 MAPK in protective effect of remifentanil preconditioning(RPC) on myocardial ischemia reperfusion injury in rat hearts.Methods Male Spargue-Dawley rats weighing 300 g to 350 g were used.They were randomly assigned to 1 of 8 groups: Control(CON,saline vehicle),SB 203580(SB,a p38 MAPK inhibitor),RPC,ischemia preconditioning(IPC),SB+RPC,SB+IPC, RPC+SB and IPC+SB.Infarct size(IS),a percentage of the area at risk(AAR),was determined by triphenyltetrazolium(TTC) staining.Tissue simple were processed from the entire AAR of left ventricle for the determination of p38 MAPK protein expression(5 hearts/group).The bands representing the proteins were visualised using an enhanced chemiluminescence detection system.Results IS/AAR was reduced by IPC or RPC,compare to CON.SB administered prior to PC abolished effect of IS/AAR reduction of IPC but RPC Treatment of SB prior to sustained ischemia diminished both protective effect of RPC and IPC on IS/AAR.In IPC group,phospho-p38 MAPK protein increased significantly within 5 min ischemia and remained elevating at 30 min reperfusion,while phospho-p38 MAPK protein in RPC increased significantly at 30 min reperfusion only.Conclusion The activation of p38 MAPK acts as a mediator of RPC,while it may have trigger and mediator effects in IPC.展开更多
早产儿脑损伤（braininjury in premature infants，BIPI）是引起早产儿认知功能障碍以及脑瘫的首要病因，严重影响早产儿的生存质量，也给家庭和社会带来沉重负担。近20年来，随着早产儿救治水平的进一步提高，超早产儿的成活率明显增...早产儿脑损伤（braininjury in premature infants，BIPI）是引起早产儿认知功能障碍以及脑瘫的首要病因，严重影响早产儿的生存质量，也给家庭和社会带来沉重负担。近20年来，随着早产儿救治水平的进一步提高，超早产儿的成活率明显增加（50％--70％），随之而来的神经发育障碍也大幅上升。大量研究显示至少有1／4的存活者发生脑瘫，而神经发育不良的发生率更是超过50％。展开更多
AIM: To detect the effect of acid fibroblast growth factor (aFGF) on apoptosis and gene expression of bax and bcl-2 gene in rat intestine after ischemia/reperfusion (I/R) injury, and to explore the protective mechanis...AIM: To detect the effect of acid fibroblast growth factor (aFGF) on apoptosis and gene expression of bax and bcl-2 gene in rat intestine after ischemia/reperfusion (I/R) injury, and to explore the protective mechanisms of aFGF.METHODS: One hundred and eight Wistar rats were randomly divided into sham-operated control group (C)(n = 6), intestinal ischemia group (I) (n = 6), aFGF treatment group (A) (n = 48) and intestinal ischemia reperfusion group (R) (n = 48). In group I, the animals were killed after 45 min of superior mesenteric artery (SMA) occlusion, while in groups R and A, the rats sustained 45 min of SMA occlusion and were then treated with normal saline and aFGF, respectively, sustained 15 min, 30 min, 1, 2, 6, 12, 24, or 48 h of reperfusion, respectively. In group C, SMA was separated, but without occlusion. Apoptosis in intestinal villus was determined with terminal deoxynucleotidyl transferase mediated dUTP-biotin nickend labeling technique (TUNEL). Intestinal tissue samples were taken not only for detection of bax and bcl-2 gene expression by RT-PCR, but also for detection of bax and bcl 2 protein expression and distribution by immunohistochemical analysis.RESULTS: The rat survival rates in aFGF treated group were higher than group R (P＜0.05) and the improvement of intestinal histological structures was observed at 2, 6, and 12 h after the reperfusion in group A compared with group R. The apoptotic rates were (41.17±3.49)%, (42.83±5.23)% and (53.33±6.92)% at 2, 6 and 12 h after reperfusion, respectively in group A, apparently less than those of group R at matched time points (50.67±6.95, 54.17±7.86, 64.33±6.47, respectively) (P＜0.05). The bax gene transcription and translation were significantly decreased in group A vs group R, while mRNA and protein contents of Bcl-2 in group A were obviously higher than those in groupR during 2-12 h period after reperfusion.CONCLUSION: The changes in histological structure and the increment of apoptotic rate indicated that the intestinal barrier was damaged after intestinal I/R injury, whilst intravenous aFGF could alleviate apoptosis induced by ischemia and reperfusion in rat intestinal tissues, in which genes of bax and bcl-2 might play important roles.展开更多
Objective. To determine the effect of albumin administration on lung injury in traumatic/hemorrhagic shock （T/HS） rats. Methods： Forty-eight adult Sprague-Dawley rats were divided into three groups randomly （ n =...Objective. To determine the effect of albumin administration on lung injury in traumatic/hemorrhagic shock （T/HS） rats. Methods： Forty-eight adult Sprague-Dawley rats were divided into three groups randomly （ n = 16 in each group） ： Group A, Group B, Group C. In Group A, rats underwent laparotomy without shock. In Group B, rats undergoing T/HS were resuscitated with their blood plus lactated Ringer＇s （twice the volume of shed blood ）. In Group C, rats undergoing T/HS were resuscitated with their shed blood plus additional 3 ml of 5% human albumin. The expression of polymorphonuclear neutrophils CD18/CD11b in jugular vein blood was evaluated. The main lung injury indexes （the activity of myeloperoxidase and lung injury score） were measured. Results： Significant differences of the expression of CD18/11b and the severity degree of lung injury were found between the three groups. （P〈0.05）. The expression of CD18/CD11b and the main lung injury indexes in Group B and Goup C incresed significantly compared with those in Group A （P 〈0.05）. At the same time, the expression of CD18/CD11b and the main lung injury indexes in Group C decreased dramatically, compared with those in Group B （ P 〈0.05 ）. Conclusions ： The infusion of albumin during resuscitation period can protect lungs from injury and decrease the expression of CD18/CD11b in T/HS rats.展开更多
文摘Objective To assess the role of p38 MAPK in protective effect of remifentanil preconditioning(RPC) on myocardial ischemia reperfusion injury in rat hearts.Methods Male Spargue-Dawley rats weighing 300 g to 350 g were used.They were randomly assigned to 1 of 8 groups: Control(CON,saline vehicle),SB 203580(SB,a p38 MAPK inhibitor),RPC,ischemia preconditioning(IPC),SB+RPC,SB+IPC, RPC+SB and IPC+SB.Infarct size(IS),a percentage of the area at risk(AAR),was determined by triphenyltetrazolium(TTC) staining.Tissue simple were processed from the entire AAR of left ventricle for the determination of p38 MAPK protein expression(5 hearts/group).The bands representing the proteins were visualised using an enhanced chemiluminescence detection system.Results IS/AAR was reduced by IPC or RPC,compare to CON.SB administered prior to PC abolished effect of IS/AAR reduction of IPC but RPC Treatment of SB prior to sustained ischemia diminished both protective effect of RPC and IPC on IS/AAR.In IPC group,phospho-p38 MAPK protein increased significantly within 5 min ischemia and remained elevating at 30 min reperfusion,while phospho-p38 MAPK protein in RPC increased significantly at 30 min reperfusion only.Conclusion The activation of p38 MAPK acts as a mediator of RPC,while it may have trigger and mediator effects in IPC.
文摘早产儿脑损伤（braininjury in premature infants，BIPI）是引起早产儿认知功能障碍以及脑瘫的首要病因，严重影响早产儿的生存质量，也给家庭和社会带来沉重负担。近20年来，随着早产儿救治水平的进一步提高，超早产儿的成活率明显增加（50％--70％），随之而来的神经发育障碍也大幅上升。大量研究显示至少有1／4的存活者发生脑瘫，而神经发育不良的发生率更是超过50％。
基金Supported by the National Basic Science and Development Programme, No. G1999054204the National Natural Science Foundation of China, No. 30170966, 30230370
文摘AIM: To detect the effect of acid fibroblast growth factor (aFGF) on apoptosis and gene expression of bax and bcl-2 gene in rat intestine after ischemia/reperfusion (I/R) injury, and to explore the protective mechanisms of aFGF.METHODS: One hundred and eight Wistar rats were randomly divided into sham-operated control group (C)(n = 6), intestinal ischemia group (I) (n = 6), aFGF treatment group (A) (n = 48) and intestinal ischemia reperfusion group (R) (n = 48). In group I, the animals were killed after 45 min of superior mesenteric artery (SMA) occlusion, while in groups R and A, the rats sustained 45 min of SMA occlusion and were then treated with normal saline and aFGF, respectively, sustained 15 min, 30 min, 1, 2, 6, 12, 24, or 48 h of reperfusion, respectively. In group C, SMA was separated, but without occlusion. Apoptosis in intestinal villus was determined with terminal deoxynucleotidyl transferase mediated dUTP-biotin nickend labeling technique (TUNEL). Intestinal tissue samples were taken not only for detection of bax and bcl-2 gene expression by RT-PCR, but also for detection of bax and bcl 2 protein expression and distribution by immunohistochemical analysis.RESULTS: The rat survival rates in aFGF treated group were higher than group R (P＜0.05) and the improvement of intestinal histological structures was observed at 2, 6, and 12 h after the reperfusion in group A compared with group R. The apoptotic rates were (41.17±3.49)%, (42.83±5.23)% and (53.33±6.92)% at 2, 6 and 12 h after reperfusion, respectively in group A, apparently less than those of group R at matched time points (50.67±6.95, 54.17±7.86, 64.33±6.47, respectively) (P＜0.05). The bax gene transcription and translation were significantly decreased in group A vs group R, while mRNA and protein contents of Bcl-2 in group A were obviously higher than those in groupR during 2-12 h period after reperfusion.CONCLUSION: The changes in histological structure and the increment of apoptotic rate indicated that the intestinal barrier was damaged after intestinal I/R injury, whilst intravenous aFGF could alleviate apoptosis induced by ischemia and reperfusion in rat intestinal tissues, in which genes of bax and bcl-2 might play important roles.
文摘Objective. To determine the effect of albumin administration on lung injury in traumatic/hemorrhagic shock （T/HS） rats. Methods： Forty-eight adult Sprague-Dawley rats were divided into three groups randomly （ n = 16 in each group） ： Group A, Group B, Group C. In Group A, rats underwent laparotomy without shock. In Group B, rats undergoing T/HS were resuscitated with their blood plus lactated Ringer＇s （twice the volume of shed blood ）. In Group C, rats undergoing T/HS were resuscitated with their shed blood plus additional 3 ml of 5% human albumin. The expression of polymorphonuclear neutrophils CD18/CD11b in jugular vein blood was evaluated. The main lung injury indexes （the activity of myeloperoxidase and lung injury score） were measured. Results： Significant differences of the expression of CD18/11b and the severity degree of lung injury were found between the three groups. （P〈0.05）. The expression of CD18/CD11b and the main lung injury indexes in Group B and Goup C incresed significantly compared with those in Group A （P 〈0.05）. At the same time, the expression of CD18/CD11b and the main lung injury indexes in Group C decreased dramatically, compared with those in Group B （ P 〈0.05 ）. Conclusions ： The infusion of albumin during resuscitation period can protect lungs from injury and decrease the expression of CD18/CD11b in T/HS rats.
