Liver regeneration is a complex and well-orchestrated process,during which hepatic cells are activated to produce large signal molecules in response to liver injury or mass reduction.These signal molecules,in turn,set...Liver regeneration is a complex and well-orchestrated process,during which hepatic cells are activated to produce large signal molecules in response to liver injury or mass reduction.These signal molecules,in turn,set up the connections and cross-talk among liver cells to promote hepatic recovery.In this review,we endeavor to summarize the network of signal molecules that mediates hepatic cell communication in the regulation of liver regeneration.展开更多
AIM： To study the effect of WeiJia on chronic liver injury using carbon tetrachloride （CCh） induced liver injury animal model. METHODS： Wistar rats weighing 180-220g were randomly divided into three groups： norma...AIM： To study the effect of WeiJia on chronic liver injury using carbon tetrachloride （CCh） induced liver injury animal model. METHODS： Wistar rats weighing 180-220g were randomly divided into three groups： normal control group （Group A）, CCh induced liver injury control group （Group B） and CCI4 induction with WeiJia treatment group （Group C）. Each group consisted of 14 rats. Liver damage and fibrosis was induced by subcutaneous injection with 40% CCh in olive oil at 3 mL/kg body weight twice a week for eight weeks for Groups B and C rats whereas olive oil was used for Group A rats. Starting from the third week, Group C rats also received daily intraperitoneal injection of Wei.lia at a dose of 1.25 μg/kg body weight. Animals were sacrificed at the fifth week （4 male, 3 female）, and eighth week （4 male, 3 female） respectively. Degree of fibrosis were measured and serological markers for liver fibrosis and function including hyaluronic acid （HA）, type Ⅳ collagen （CIV）, γ-glutamyl transferase （γ-GT）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were determined. Alpha smooth muscle actin （α-SMA） and proliferating cell nuclear antigen （PCNA） immunohistochemistry were also performed. RESULTS： CCl4 induction led to the damage of liver and development of fibrosis in Group B and Group C rats when compared to Group A rats. The treatment of WeiJia in Group C rats could reduce the fibrosis condition significantly compared to Group B rats. The effect could be observed after three weeks of treatment and was more obvious after eight weeks of treatment. Serum HA, CIV,ALT, AST and γ-GT levels after eight weeks of treatment for Group C rats were 58±22 μg/L （P〈0.01）, 57±21 μg/L （P〈0.01）, 47±10 U/L （P〈0.01）, 139±13 U/L （P〈0.05） and 52±21 U/L （P〉0.05） respectively, similar to normal control group （Group A）, but significantly different from CCh induced liver injury control group （Group B）. An increase in PCNA and decrease in α-SMA expression level was also observed. CONCLUSION： WeiJia could improve liver function and reduce liver fibrosis which might be through the inhibition of stellate cell activity.展开更多
It has been shown that long-lasting noxious inputs can alter the expression of several genes in the spinal dorsal horn, including the elevations in the amount of Fos protein and dynorphin (Dyn) peptides. However, the ...It has been shown that long-lasting noxious inputs can alter the expression of several genes in the spinal dorsal horn, including the elevations in the amount of Fos protein and dynorphin (Dyn) peptides. However, the roles of Dyn A, a main product of posttranslation processing of prodynorphin, and c-fos expression in spinal nociceptive modulation are still controversial. In order to clarify further the relationships among the展开更多
OBJECTIVE: To evaluate the influence of necrotic tissue on progressive injury in deep partial thickness burn wounds. METHODS: Tissue specimens were cultured both for estimation of IL-8, EGF, bFGF, PDGF-AB and histopat...OBJECTIVE: To evaluate the influence of necrotic tissue on progressive injury in deep partial thickness burn wounds. METHODS: Tissue specimens were cultured both for estimation of IL-8, EGF, bFGF, PDGF-AB and histopathological examination, from the pre-operation, post-operation, and non-operation wounds from seven patients with deep partial thickness burn. RESULTS: In seven specimens from the non-operation group, IL-8 release increased compared with those in the post-operation group (P展开更多
Objective： To investigate the influence of moxibustion products on mitochondrial transmembrane potential （MTP） and mRNA expression of Bax/Bcl-2 in alveolar type Ⅱ epithelial A549 cells, and to further explore infl...Objective： To investigate the influence of moxibustion products on mitochondrial transmembrane potential （MTP） and mRNA expression of Bax/Bcl-2 in alveolar type Ⅱ epithelial A549 cells, and to further explore influence of moxibustion products on the oxidative damage of A549 cells. Methods： Smoke and particles generated by moxibustion were collected using the filter box for gas sampling. The moxa smoke extract （MSE） was diluted sequentially to the final concentrations of 0.05 mg/mL, 0.2 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL using the cell culture medium, and A549 cells were then intervened by the above MSE solution. Cell MTP was detected by JC-1 staining. Fluorescence quantitative polymerase chain reaction （PCR） was used to detect Bax/Bcl-2 mRNA expression of A549 cells. Results： Compared with cells in the normal control group, MTP was significantly decreased in cells of 0.3 mg/mL and 0.4 mg/mL MSE intervention groups （P〈0.01）; while MTP showed no significant changes in cells of 0.05 mg/mL, 0.1 mg/mL and 0.2 mg/mL MSE intervention groups （P〉0.05）; compared with cells in 0.05 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL MSE intervention groups （P〈0.05）; compared with cells in 0.1 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.4 mg/mL MSE intervention group （P〈0.01）. Bax mRNA expression of cells in each concentration of MSE intervention group all showed no significant difference compared to that in the normal control group; Bcl-2 mRNA expression of cells was reduced with the increase of MSE intervention concentration. Wherein, Bcl-2 mRNA expressions of cells in 0.4 mg/mL and 0.3 mg/mL MSE intervention groups were significantly reduced compared with that of cells in the normal control group （P〈0.05）; Bcl-2 mRNA expression of cells in 0.4 mg/mL MSE intervention group was significantly reduced compared to that in 0.05 mg/mL MSE intervention group （P〈0.05）. Conclusion： Certain higher concentration of moxa smoke could reduce MTP and mRNA expression of the anti-apoptosis gene Bcl-2 in alveolar type Ⅱ epithelial A549 cells. Oxidative damage may be the important mechanism of apoptosis caused by the high concentration of moxa smoke solution, and further studies are necessary on the specific mechanisms.展开更多
Objective: To determine the effects of recombinant soluble complement receptor type I (sCR1) on the immune inflammatory reaction in acute spinal cord injury tissue of rats and its protective effects. Methods: SD rat m...Objective: To determine the effects of recombinant soluble complement receptor type I (sCR1) on the immune inflammatory reaction in acute spinal cord injury tissue of rats and its protective effects. Methods: SD rat models of acute spinal cord injury were prepared by modified Allen’s method. The motor function of the rat lower extremities in sCR1 group and normal saline (NS) group was evaluated by the tiltboard experiment at 12 h, 1 d, 3 d, 7 d, and 14 d. The neutrophil infiltration and C3c positive expression were observed. The myeloperoxidase activity was assessed in the injury tissue at 12 h, 1 d, 3 d, 7 d, and 14 d after injury in the two groups. Results: The motor function of rat in sCR1 group at 3 d, 7 d, and 14 d was obviously better than that in NS group (P< 0.01, P< 0.01, P< 0.01). C3c positive expression in sCR1 group at each time point after injury was obviously less than that in NS group (P< 0.01). The myeloperoxidase activity in sCR1 group at each time point after injury was obviously less than that in NS group (P< 0.01). Conclusions: Recombinant soluble complement receptor type I (sCR1) can lessen the immune inflammatory reaction in acute spinal cord injury tissue and relieve secondary spinal cord injury by inhibiting the activation of the complement system.展开更多
文摘Liver regeneration is a complex and well-orchestrated process,during which hepatic cells are activated to produce large signal molecules in response to liver injury or mass reduction.These signal molecules,in turn,set up the connections and cross-talk among liver cells to promote hepatic recovery.In this review,we endeavor to summarize the network of signal molecules that mediates hepatic cell communication in the regulation of liver regeneration.
基金Supported by Innovation and Technology Fund of the Hong Kong SAR Government(UIM/101)the National Hi-Tech 863 Program of the Ministry of Science and Technology of China,2003AA2Z2052
文摘AIM： To study the effect of WeiJia on chronic liver injury using carbon tetrachloride （CCh） induced liver injury animal model. METHODS： Wistar rats weighing 180-220g were randomly divided into three groups： normal control group （Group A）, CCh induced liver injury control group （Group B） and CCI4 induction with WeiJia treatment group （Group C）. Each group consisted of 14 rats. Liver damage and fibrosis was induced by subcutaneous injection with 40% CCh in olive oil at 3 mL/kg body weight twice a week for eight weeks for Groups B and C rats whereas olive oil was used for Group A rats. Starting from the third week, Group C rats also received daily intraperitoneal injection of Wei.lia at a dose of 1.25 μg/kg body weight. Animals were sacrificed at the fifth week （4 male, 3 female）, and eighth week （4 male, 3 female） respectively. Degree of fibrosis were measured and serological markers for liver fibrosis and function including hyaluronic acid （HA）, type Ⅳ collagen （CIV）, γ-glutamyl transferase （γ-GT）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were determined. Alpha smooth muscle actin （α-SMA） and proliferating cell nuclear antigen （PCNA） immunohistochemistry were also performed. RESULTS： CCl4 induction led to the damage of liver and development of fibrosis in Group B and Group C rats when compared to Group A rats. The treatment of WeiJia in Group C rats could reduce the fibrosis condition significantly compared to Group B rats. The effect could be observed after three weeks of treatment and was more obvious after eight weeks of treatment. Serum HA, CIV,ALT, AST and γ-GT levels after eight weeks of treatment for Group C rats were 58±22 μg/L （P〈0.01）, 57±21 μg/L （P〈0.01）, 47±10 U/L （P〈0.01）, 139±13 U/L （P〈0.05） and 52±21 U/L （P〉0.05） respectively, similar to normal control group （Group A）, but significantly different from CCh induced liver injury control group （Group B）. An increase in PCNA and decrease in α-SMA expression level was also observed. CONCLUSION： WeiJia could improve liver function and reduce liver fibrosis which might be through the inhibition of stellate cell activity.
文摘It has been shown that long-lasting noxious inputs can alter the expression of several genes in the spinal dorsal horn, including the elevations in the amount of Fos protein and dynorphin (Dyn) peptides. However, the roles of Dyn A, a main product of posttranslation processing of prodynorphin, and c-fos expression in spinal nociceptive modulation are still controversial. In order to clarify further the relationships among the
基金supported by the Major State Basic Research Development Program of China(No．Gl999054205)．
文摘OBJECTIVE: To evaluate the influence of necrotic tissue on progressive injury in deep partial thickness burn wounds. METHODS: Tissue specimens were cultured both for estimation of IL-8, EGF, bFGF, PDGF-AB and histopathological examination, from the pre-operation, post-operation, and non-operation wounds from seven patients with deep partial thickness burn. RESULTS: In seven specimens from the non-operation group, IL-8 release increased compared with those in the post-operation group (P
基金Scientific Research Project of Shanghai Health Bureau(No.20134239)National Natural Science Foundation of China(No.81102637)National Basic Research Program of China(973 Program,No.2009CB522900)~~
文摘Objective： To investigate the influence of moxibustion products on mitochondrial transmembrane potential （MTP） and mRNA expression of Bax/Bcl-2 in alveolar type Ⅱ epithelial A549 cells, and to further explore influence of moxibustion products on the oxidative damage of A549 cells. Methods： Smoke and particles generated by moxibustion were collected using the filter box for gas sampling. The moxa smoke extract （MSE） was diluted sequentially to the final concentrations of 0.05 mg/mL, 0.2 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL using the cell culture medium, and A549 cells were then intervened by the above MSE solution. Cell MTP was detected by JC-1 staining. Fluorescence quantitative polymerase chain reaction （PCR） was used to detect Bax/Bcl-2 mRNA expression of A549 cells. Results： Compared with cells in the normal control group, MTP was significantly decreased in cells of 0.3 mg/mL and 0.4 mg/mL MSE intervention groups （P〈0.01）; while MTP showed no significant changes in cells of 0.05 mg/mL, 0.1 mg/mL and 0.2 mg/mL MSE intervention groups （P〉0.05）; compared with cells in 0.05 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL MSE intervention groups （P〈0.05）; compared with cells in 0.1 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.4 mg/mL MSE intervention group （P〈0.01）. Bax mRNA expression of cells in each concentration of MSE intervention group all showed no significant difference compared to that in the normal control group; Bcl-2 mRNA expression of cells was reduced with the increase of MSE intervention concentration. Wherein, Bcl-2 mRNA expressions of cells in 0.4 mg/mL and 0.3 mg/mL MSE intervention groups were significantly reduced compared with that of cells in the normal control group （P〈0.05）; Bcl-2 mRNA expression of cells in 0.4 mg/mL MSE intervention group was significantly reduced compared to that in 0.05 mg/mL MSE intervention group （P〈0.05）. Conclusion： Certain higher concentration of moxa smoke could reduce MTP and mRNA expression of the anti-apoptosis gene Bcl-2 in alveolar type Ⅱ epithelial A549 cells. Oxidative damage may be the important mechanism of apoptosis caused by the high concentration of moxa smoke solution, and further studies are necessary on the specific mechanisms.
文摘Objective: To determine the effects of recombinant soluble complement receptor type I (sCR1) on the immune inflammatory reaction in acute spinal cord injury tissue of rats and its protective effects. Methods: SD rat models of acute spinal cord injury were prepared by modified Allen’s method. The motor function of the rat lower extremities in sCR1 group and normal saline (NS) group was evaluated by the tiltboard experiment at 12 h, 1 d, 3 d, 7 d, and 14 d. The neutrophil infiltration and C3c positive expression were observed. The myeloperoxidase activity was assessed in the injury tissue at 12 h, 1 d, 3 d, 7 d, and 14 d after injury in the two groups. Results: The motor function of rat in sCR1 group at 3 d, 7 d, and 14 d was obviously better than that in NS group (P< 0.01, P< 0.01, P< 0.01). C3c positive expression in sCR1 group at each time point after injury was obviously less than that in NS group (P< 0.01). The myeloperoxidase activity in sCR1 group at each time point after injury was obviously less than that in NS group (P< 0.01). Conclusions: Recombinant soluble complement receptor type I (sCR1) can lessen the immune inflammatory reaction in acute spinal cord injury tissue and relieve secondary spinal cord injury by inhibiting the activation of the complement system.
