Objective ：To investigate the feasibility of using natural poritos as scaffolds in bone tissue engineering （TE） and repair of caprine mandibular segmental defect with titanium reticulum reinforced. Methods： Natur...Objective ：To investigate the feasibility of using natural poritos as scaffolds in bone tissue engineering （TE） and repair of caprine mandibular segmental defect with titanium reticulum reinforced. Methods： Natural poritos with a pore of 190-230 μm in size and porosity of about 50 %-65 % was molded into the shape of granules 5 mm × 5 mm × 5 mm in size. Expanded autologous caprine marrow mesenchymal stem cells were induced by recombinant human morphogenetic protein-2 （rhBMP2） to improve osteoblastic phenotype. Then marrow derived osteoblasts were seeded into poritos in density of 4 × 10^7/ml and incubated in vitro for 48 hours prior to implantation. Then osteoblastic cells/poritos complexes were implanted into mandibular defect and the defect was reinforced by titanium reticulum. Implantation of poritos alone acted as the control. Bone regeneration was assessed 4, 8, 16 weeks after implantation using roentgenographie analysis and histological observation was done after 16 weeks. Results： New bone could be observed histologically on the surface and in the pores of natural coral in all specimens in the cell-seeding group, whereas in the control group there was no evidence of osteogenesis process in the center of the construction. The results showed that new bone grafts were successfully restored 16 weeks after implantation. Conclusions： This study suggests the feasibility of using porous coral as scaffold material transplanted with marrow derived osteoblasts by TE method. By means of titanium reticulum reinforcement, mandibular defect could be successfully restored. It shows the potentiality of using this method for the reconstruction of bone defect in cfinic.展开更多
Great progress has been made in study on dynamic behavior of the damaged structures subject to deterministic excitation.The stochastic response analysis of the damaged structures,however,has not yet attracted people&#...Great progress has been made in study on dynamic behavior of the damaged structures subject to deterministic excitation.The stochastic response analysis of the damaged structures,however,has not yet attracted people's attention.Taking the damaged elastic beams for example,the analysis procedure for stochastic response of the damaged structures subject to stochastic excitations is investigated in this paper.First,the damage constitutive relations and the corresponding damage evolution equation of one-dimensional elastic structures are briefly discussed.Second,the stochastic dynamic equation with respect to transverse displacement of the damaged elastic beams is deduced.The finite difference method and Newmark method are adopted to solve the stochastic partially-differential equation and corresponding boundary conditions.The stochastic response characteristic,damage evolution law,the effect of noise intensity on damage evolution and the first-passage time of damage are discussed in detail.The present work extends the research field of damaged structures,and the proposed procedure can be generalized to analyze the dynamic behavior of more complex structures,such as damaged plates and shells.展开更多
文摘Objective ：To investigate the feasibility of using natural poritos as scaffolds in bone tissue engineering （TE） and repair of caprine mandibular segmental defect with titanium reticulum reinforced. Methods： Natural poritos with a pore of 190-230 μm in size and porosity of about 50 %-65 % was molded into the shape of granules 5 mm × 5 mm × 5 mm in size. Expanded autologous caprine marrow mesenchymal stem cells were induced by recombinant human morphogenetic protein-2 （rhBMP2） to improve osteoblastic phenotype. Then marrow derived osteoblasts were seeded into poritos in density of 4 × 10^7/ml and incubated in vitro for 48 hours prior to implantation. Then osteoblastic cells/poritos complexes were implanted into mandibular defect and the defect was reinforced by titanium reticulum. Implantation of poritos alone acted as the control. Bone regeneration was assessed 4, 8, 16 weeks after implantation using roentgenographie analysis and histological observation was done after 16 weeks. Results： New bone could be observed histologically on the surface and in the pores of natural coral in all specimens in the cell-seeding group, whereas in the control group there was no evidence of osteogenesis process in the center of the construction. The results showed that new bone grafts were successfully restored 16 weeks after implantation. Conclusions： This study suggests the feasibility of using porous coral as scaffold material transplanted with marrow derived osteoblasts by TE method. By means of titanium reticulum reinforcement, mandibular defect could be successfully restored. It shows the potentiality of using this method for the reconstruction of bone defect in cfinic.
基金supported by the National Natural Science Foundation of China (Grant No. 11072076)
文摘Great progress has been made in study on dynamic behavior of the damaged structures subject to deterministic excitation.The stochastic response analysis of the damaged structures,however,has not yet attracted people's attention.Taking the damaged elastic beams for example,the analysis procedure for stochastic response of the damaged structures subject to stochastic excitations is investigated in this paper.First,the damage constitutive relations and the corresponding damage evolution equation of one-dimensional elastic structures are briefly discussed.Second,the stochastic dynamic equation with respect to transverse displacement of the damaged elastic beams is deduced.The finite difference method and Newmark method are adopted to solve the stochastic partially-differential equation and corresponding boundary conditions.The stochastic response characteristic,damage evolution law,the effect of noise intensity on damage evolution and the first-passage time of damage are discussed in detail.The present work extends the research field of damaged structures,and the proposed procedure can be generalized to analyze the dynamic behavior of more complex structures,such as damaged plates and shells.
