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老年高血压病患者血清血管紧张素转换酶活性观察 被引量:1
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作者 洪华山 王一波 +1 位作者 吴青 蓝玉福 《福建医药杂志》 CAS 1996年第6期5-6,共2页
应用分光光度法测定15例并内皮损伤、28例无内皮损伤老年高血压病患者血清血管紧张素转换酶(SACE)水平,并以131例正常人作对照。结果内皮损伤组、内皮无损伤组的SACE各为137±38.5u、294±57.... 应用分光光度法测定15例并内皮损伤、28例无内皮损伤老年高血压病患者血清血管紧张素转换酶(SACE)水平,并以131例正常人作对照。结果内皮损伤组、内皮无损伤组的SACE各为137±38.5u、294±57.8u;分别低于和高于正常对照组(244±82.5u)。说明老年高血压病患者的SACE活性随着并发血管内皮细胞损伤的有无而表现下降或升高。 展开更多
关键词 老年人 高血压 血管紧张素 换转酶 内皮细胞
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猪带绦虫囊尾蚴囊液谷胱甘肽S转换酶活性测定
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作者 孙新 胡守锋 +3 位作者 陈兴智 夏惠 方强 陈兴保 《蚌埠医学院学报》 CAS 2001年第1期1-2,共2页
目的:探讨猪带绦虫囊尾蚴囊液内谷胱甘肽S转换酶(GST)的活性。方法:测定酶催化反应后底物浓度的变化,计算GST活力单位。结果:囊液经1∶20稀释,重复4次测定酶促管与非酶促管吸光值,测得平均GST活性为61.84活力单位;囊液冻干品则几... 目的:探讨猪带绦虫囊尾蚴囊液内谷胱甘肽S转换酶(GST)的活性。方法:测定酶催化反应后底物浓度的变化,计算GST活力单位。结果:囊液经1∶20稀释,重复4次测定酶促管与非酶促管吸光值,测得平均GST活性为61.84活力单位;囊液冻干品则几无GST活性。结论:囊液中存在游离形式的GST活性,对筛选保护性抗原和可能的化疗靶分子具有潜在意义。 展开更多
关键词 囊尾蚴 谷胱甘肽S 活化 GST 囊液
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Effect of L158,809 and Cilazapril on the Expression of TGF-β_1 and Secretion of Extracellular Matrix Proteins in Cultured Human Mesangial Cells
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作者 杨涛 陈家伟 +2 位作者 刘超 刘翠萍 覃又文 《Journal of Nanjing Medical University》 2003年第6期288-293,共6页
Objective: To explore the effect of L158, 809 (angiatensin Ⅱ receptorMockers, ARBs) and Cilazapril (Angiotensin converting enzyme inhibitor, ACEI) on the expression oftransforming growth factor-β_1 (TGF-β_1) and se... Objective: To explore the effect of L158, 809 (angiatensin Ⅱ receptorMockers, ARBs) and Cilazapril (Angiotensin converting enzyme inhibitor, ACEI) on the expression oftransforming growth factor-β_1 (TGF-β_1) and secretion of fibronectin, laminin and type Ⅳcollagen from the cultured human mesangial cells . Methods: Human mesangial cells were cultured indifferent glucose (5.6 mmol/L and 30 mmol/L) and agents (1, 10, 100 and 500 μmol/L) concentrations. The proliferation of mesangial cells were detected at 24, 48 and 72 h . Then the mesangial cellsare divided into four groups, low glucose (5.6 mmol/L) control group, high glucose (30 mmol/L)control group , L158, 809 (10 μmol/L) group and cilazapril (10 μmol/L) group. Forty- eight hourslater, the expression of TGF-β_1 were detected by RT-PCR. Concentrations of TGF-β_1 ,fibronection, laminin and type Ⅳ collagen in the su-pematants of the, mesangial cells weredetermined by EUSA and radioimmunoassay methods. Results: Compared with low glucose control group,the mesangial cells under high glucose medium show excessive proliferation and more TGF-β_1,fibronectin, laminin and type Ⅳ collagen in the supernatant. The expression of TGF-β_1 mRNA wasalso significantly increased under high glucose. The levels of TGF-β_1 and ECM (extracellularmatrix) proteins in the L158, 809 group and cilazapril group are obviously lower than that of thehigh glucose control group. The expression of TGF-β_1 mRNA was markedly decreased in the L158, 809group and cilazapril group compared with that of high glucose control group . Conclusion: Highglucose stimulated the cultured human mesangial cells to excessively proliferate, express TGF-β_1and secrete ECM proteins, and the high glucose-indeced changes were suppressed by either L158, 809and cilazapril. 展开更多
关键词 angiotensin receptor blocker L158 809 ECM protein mesangial cells
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SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway 被引量:51
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作者 Wang,H Yang,P +4 位作者 Liu,K Guo,F Zhang,Y Zhang,G Jiang,C 《Cell Research》 SCIE CAS CSCD 2008年第2期290-301,共12页
While severe acute respiratory syndrome coronavirus (SARS-CoV)~as initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that yirus entry may also involve endo... While severe acute respiratory syndrome coronavirus (SARS-CoV)~as initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that yirus entry may also involve endocytosis. We have found that SARS-CoV enters cells viapH- and receptor-dependent endocytosis. Treatment of cells with either SARS-COV spike protein or spike-bearing pseudoviruses resulted in the translocation of angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, from the cell surface to endosomes. In addition, the spike-bearing pseudoviruses and early endosome antigen 1 were found to colocalize in endosomes. Further analyses using specific endocytic path- way inhibitors and dominant-negative Epsl5 as well as caveolin-1 colocalization study suggested that virus entry was mediated by a clathrin- and caveolae-independent mechanism. Moreover, cholesterol- and sphingolipid-rich lipid raft microdomains in the plasma membrane, which have been shown to act as platforms for many physiological signaling pathways, were shown to be involved in virus entry. Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research. 展开更多
关键词 Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) ENDOCYTOSIS angiotensin-converting enzyme 2 (ACE2) lipid rafts
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Correlation and prognostic significance of beta-galactoside alpha-2,6-sialyltransferase and serum monosialylated alpha-fetoprotein in hepatocellular carcinoma 被引量:4
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作者 Terence CW Poon Clarissa HS Chiu +5 位作者 Paul BS Lai Tony SK Mok Benny Zee Anthony TC Chan Joseph JY Sung Philip J Johnson 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第42期6701-6706,共6页
AIM: To investigate the correlation between tissue ST6Gal I and serum msAFP in HCC patients, and to investigate their prognostic significance. METHODS: Preoperative sera, paired tumorous and non-tumorous tissues wer... AIM: To investigate the correlation between tissue ST6Gal I and serum msAFP in HCC patients, and to investigate their prognostic significance. METHODS: Preoperative sera, paired tumorous and non-tumorous tissues were collected from 19 consecutive patients who had undergone surgical resection of HCC. ST6Gal I activities in the tissues were measured by an in vitro microsomal enzyme activity assay. The percentages of tumor-specific msAFP in the sera were also estimated by an isoelectric focusing-immunoblotting assay. RESULTS: The tumor ST6Gal I activity was negatively correlated with serum msAFP percentage (r = -0.53, P = 0.019). Both decreased tumor ST6Gal I activity and increased serum msAFP percentage were associated with poor tumor cell differentiation. Univariate analyses showed that both decreased tumor ST6Gal I activity (P = 0.028), increased serum msAFP percentage (P = 0.034) and poor tumor cell differentiation (P = 0.031)were associated with shorter overall survival. Multivariate analysis using the Cox regression model showed that the preoperative serum msAFP percentage (P = 0.022) and tumor cell differentiation status (P = 0.048) were independent prognostic indicators for patient overall survival. CONCLUSION: Our results indicate that the presence of msAFP in blood circulation is associated with a decreased activity of ST6Gal I activity in HCC. Both tissue ST6Gal I and serum msAFP are potential prognostic markers for patients with operable HCC. 展开更多
关键词 ALPHA-FETOPROTEIN Beta-galactoside alpha-2 6-sialyltransferase Hepatocellular carcinoma Patientsurvival Cell differentiation
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Cardiovascular and cerebrovascular outcomes in elderly hypertensive patients treated with either ARB or ACEI 被引量:5
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作者 Cong Ma Jian Cao +4 位作者 Xue-Chun Lu Xin-Hong Guo Yan Gao Xian-Feng Liu Li Fan 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2012年第3期252-257,共6页
Background Although angiotensin converting enzyme inhibitors (ACEI) and angiotensin receptor blockers (ARB) are equally important in the treatment of hypertension, there is less evidence whether they have equal ca... Background Although angiotensin converting enzyme inhibitors (ACEI) and angiotensin receptor blockers (ARB) are equally important in the treatment of hypertension, there is less evidence whether they have equal cardiovascular and cerebrovascular protective effects, especially in elder hypertensive patients. This study aims to clarify this unresolved issue. Methods This cross-sectional study included clinical data on 933 aged male patients with hypertension who received either an ARB or ACEI for more than two months between January 2007 and May 2011. The primary outcome was the composite of cardiovascular death, non-fatal myocardial infarction, and non-fatal stroke. The secondary endpoints were unstable angina, new atrial fibrillation, and transient ischemic attack. Results The median follow-up time was 24 months. Age, drug types, cerebral infarction history, renal dysfunction history were the independent predictors of the primary endpoint. The risk of an occurrence of a primary endpoint event was higher in the ARB group than the ACEI group [P = 0.037, hazard ratios (HR): 2.124, 95% confidence interval (95% CI): 1.048-4.306]. The Kaplan-Meier method also suggests that the rate of primary endpoint occurrence was higher in the ARB group than the ACEI group (P = 0.04). In regard to the secondary endpoints, there were no significant differences between the two treatment arms (P = 0.137, HR: 1.454, 95% CI: 0.888-2.380). Patient age and coronary heart disease history were independent predictors of the secondary endpoint. Conclusion ACEI were more effective than ARB in reducing cardiovascular and cerebrovascular morbidity and mortality in aged patients with hypertension. 展开更多
关键词 Angiotensin receptor blocker Angiotensin converting enzyme inhibitor Renin-angiotensin-aldosterone system Angiotensintype 2 receptor
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Inappropriate use of digoxin in patients presenting with digoxin toxicity 被引量:2
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作者 Mustafa Adem Tatlisu Kazim Serhan Ozcan +3 位作者 Baris Gungor Ahmet Zengin Mehmet Baran Karatas Zekeriya Nurkalem 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2015年第2期143-146,共4页
Background Digoxin remains widely used today despite its narrow therapeutic index and toxicity. The objective of this study was to investigate the percentage of inappropriate use of digoxin and long-term outcomes of e... Background Digoxin remains widely used today despite its narrow therapeutic index and toxicity. The objective of this study was to investigate the percentage of inappropriate use of digoxin and long-term outcomes of elderly patients hospitalized for digoxin toxicity. Methods The study included 99 consecutive patients hospitalized for digoxin toxicity. The other study criteria for the inappropriate use of digoxin was regarded if participants having depressed left ventricular systolic function (ejection fraction 〈 45%) who were not on optimal medical therapy including beta-blocker and angiotensin-converting-enzyme inhibitor therapy or if participants having permanent AF who were not on optimal beta-blocker therapy. Results Appropriate digoxin usage was confirmed in 33 of patients in spite of its narrow therapeutic index. A total of 16 of 99 patients died, with a mean follow-up time of 22.1 ± 10.3 months. Conclusions Contrary to popular belief, the rate of inappropriate digoxin usage remains high. On account of its narrow therapeutic index and toxicity, digoxin should be used more carefully according to the current evidence and guidelines. 展开更多
关键词 DIGOXIN Ejection fraction INDICATIONS TOXICITY
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Purification and Characterization of Angiotensin I Converting Enzyme Inhibition Peptides from Sandworm Sipunculus nudus 被引量:5
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作者 SUN Xueping WANG Man +1 位作者 LIU Buming SUN Zhenliang 《Journal of Ocean University of China》 SCIE CAS CSCD 2017年第5期911-915,共5页
Three angiotensin I converting enzyme(ACE) inhibition peptides were isolated from sandworm Sipunculus nudus protein hydrolysate prepared using protamex. Consecutive purification methods, including size exclusion chrom... Three angiotensin I converting enzyme(ACE) inhibition peptides were isolated from sandworm Sipunculus nudus protein hydrolysate prepared using protamex. Consecutive purification methods, including size exclusion chromatography and reverse-phase high performance liquid chromatography(RP-HPLC), were used to isolate the ACE inhibition peptides. The amino acid sequences of the peptides were identified as Ile-Asn-Asp, Val-Glu-Pro-Gly and Leu-Ala-Asp-Glu-Phe. The IC_(50) values of the purified peptides for ACE inhibition activity were 34.72 μmol L^(-1), 20.55 μmol L^(-1) and 22.77 μmol L^(-1), respectively. These results suggested that S. nudus proteins contain specific peptides that can be released by enzymatic hydrolysis. This study may provide an experimental basis for further systematic research, rational development and clinical utilization of sandworm resources. 展开更多
关键词 hydrolysis converting purification exclusion Angiotensin Inhibition shrimp isolate purified Enzyme
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The impact of optimal medical therapy at discharge on mortality in patients with coronary artery disease 被引量:1
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作者 Shi-Jian CHEN Wei LIU +5 位作者 Bao-Tao HUANG Jia-Yu TSAUO Xiao-Bo PU Yong PENG Mao CHEN De-Jia HUANG 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2017年第2期100-107,共8页
Objective To analyze the current usage of optimal medical therapy (OMT), influencing factors, and the predictive value of OMT for all-cause mortality in coronary artery disease (CAD) patients with different subgro... Objective To analyze the current usage of optimal medical therapy (OMT), influencing factors, and the predictive value of OMT for all-cause mortality in coronary artery disease (CAD) patients with different subgroups. Methods A total of 3176 CAD patients confirmed by coronary angiography were included. OMT was defined as the combination of anti-platelet drugs, statins, beta blockers, and angiotensin converting enzyme inhibitors or angiotensin receptor blockers. Factors for OMT and its prognostic value were analyzed in CAD patients across different subgroups. Results Out of 3176 patients, only 39.8% (n = 1265) were on OMT at discharge. Factors associated with OMT at discharge were pre-admission OMT and discharge department. All-cause mortality occurred in 6.8% (n = 217) of patients. Multivariate analyses indicated that OMT was significantly associated with reduced all-cause mortality (HR: 0.65, 95% CI: 0.45~0.95; P = 0.025). Sub-group analyses indicate that male acute coronary syndrome (ACS) patients were more likely to receive survival benefits with OMT at discharge. The positive impact of OMT at discharge was more apparent after 24 months, regardless of revascularization therapy. Four-drug combination of OMT was superior to 3-drug combination therapy in ACS patients but not in stable patients. Conclusions OMT was asso- ciated with significant improvement in survival in patients with CAD. The positive impact of OMT was distinct in the CAD patients with different characteristics. 展开更多
关键词 Coronary artery disease Optimal medical therapy PROGNOSIS
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Pharmarcogenetic Mechanism of ACE liD Polymorphism Adversely Responding to ACE Inhibitors in Regulating the ACE Promoter Activity in Neurons 被引量:1
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作者 Yuan-Han Yang Hsueh-Wei Chang +4 位作者 Wei-Chiao Chang Yu-Cheng Shih Ke-Li Tsai I Chien Shyh-Jong Wu 《Journal of Pharmacy and Pharmacology》 2016年第8期419-431,共13页
The ACE (angiotensin converting enzyme) inhibitors are not only drugs widely prescribed drugs in cardiovascular diseases, but also potentially therapeutic agents in dementia. Based on the findings that the ACE inhib... The ACE (angiotensin converting enzyme) inhibitors are not only drugs widely prescribed drugs in cardiovascular diseases, but also potentially therapeutic agents in dementia. Based on the findings that the ACE inhibitors could activate the c-Jun N-terminal kinase signal to increase the ACE gene expression and that the Alu element of the human ACE gene involved in regulating ACE promoter activity, we aimed to investigate whether there are different pharmacogenetic responses of ACE I/D polymorphism to the ACE inhibitors in neurons. The three reporter vectors, pACEpro(0-SEAP, p-I-ACEpro-SEAP, and p-D-ACEpro-SEAP were used to examine the transcriptional activity of the vectors responding to the lisinopril treatment using a transient-transfection method in SH-SY5Y cells. Our results showed that lisinopril increased the promoter activity of an ACE gene by 16.7%. Additionally, we found the lisinopril enhanced the ACE promoter activity of the I-form vector by 17.2%, but adversely reduced that of the D-form vector by 16.8%, as compared with the respective control without the lisinopril treatment. Firstly, our findings had proved that the UD polymorphism of ACE gene contrarily responds to the ACE inhibitors in regulating the ACE expression in neurons, which provide a novel insight suggesting genetic testing to tailor the treatment regimens in AD (Alzheimer's disease) patients. 展开更多
关键词 ACE inhibitors ACE I/D polymorphism Aizheimer's disease PHARMACOGENETICS promoter activity.
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Raf kinase inhibitory protein: a signal transduction modulator and metastasis suppressor 被引量:10
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作者 Alexey E Granovsky Marsha Rich Rosner 《Cell Research》 SCIE CAS CSCD 2008年第4期452-457,共6页
Cells have a multitude of controls to maintain their integrity and prevent random switching from one biological state to another. Raf Kinase Inhibitory Protein (RKIP), a member of the phosphatidylethanolamine bindin... Cells have a multitude of controls to maintain their integrity and prevent random switching from one biological state to another. Raf Kinase Inhibitory Protein (RKIP), a member of the phosphatidylethanolamine binding protein (PEBP) family, is representative of a new class of modulators of signaling cascades that function to maintain the “yin yang” or balance of biological systems. RKIP inhibits MAP kinase (Raf-MEK-ERK), G protein-coupled receptor (GPCR) kinase and NFkB signaling cascades. Because RKIP targets different kinases dependent upon its state ofphosphorylation, RKIP also acts to integrate crosstalk initiated by multiple environmental stimuli. Loss or depletion of RKIP results in disruption of the normal cellular stasis and can lead to chromosomal abnormalities and disease states such as cancer. Since RKIP and the PEBP family have been reviewed previously, the goal of this analysis is to provide an update and highlight some of the unique features of RKIP that make it a critical player in the regulation of cellular signaling processes. 展开更多
关键词 Raf Kinase METASTASIS signal transduction INHIBITOR
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Left ventricular hypertrophy in relation to systolic blood pressure and the angiotensin converting enzyme I/D polymorphism in Chinese
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作者 Alexander P. Headley Yan Li Yi Zhang Ji-Yong Ge Qi-Fang Huang Ji-Guang Wang 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2009年第3期131-136,共6页
Objective There is little population-based data on the prevalence and the environmental or genetic determinants of left ventricular hypertrophy (LVH) in China. The purpose of this paper is to study LVH in relation t... Objective There is little population-based data on the prevalence and the environmental or genetic determinants of left ventricular hypertrophy (LVH) in China. The purpose of this paper is to study LVH in relation to systolic blood pressure and the angiotensin converting enzyme (ACE) insertion/deletion(I/D) polymorphism in Chinese. Methods We recorded 12- lead ECG (CardioSoft, v4.2) in 1365 residents in the Jingning County, Zhejiang Province, China. LVH was defined according to the gender-specific Sokolow-Lyon and Cornell product ECG criteria. Results Regardless of whether the Sokolow-Lyon or Cornell product ECG criteria was used, the prevalence of LVH (20.7% and 4.8%, respectively) significantly (P〈0.0001) increased with male gender (odds ratio [OR] 2.33 and 7.15) and systolic blood pressure (per 10 mm Hg increase, OR 1.46 and 1.33). If the Sokolow-Lyon criteria was used, the prevalence of LVH was also influenced by alcohol intake (OR 1.44, P=-0.03) and body mass index (OR 0.83, P=0.0005). The association between the Sokolow-Lyon voltage amplitude and the ACE I/D polymorphism was dependent on antihypertensive therapy (P=0.01). In 1262 untreated subjects, but not 103 patients on antihypertensive medication, the ACE DD compared with II subjects had significantly higher Sokolow-Lyon voltage amplitudes (29.8:-0.6 vs. 28.0-3:0.5 mV, P=-0.02) and higher risk of LVH (OR 1.74, 95% CI: 1.12-2.69, P=-0.01). Conclusion LVH is prevalent in Chinese, and is associated with systolic blood pressure and the ACE D allele. The genetic association might be modulated by antihypertensive therapy(J Geriatr Cardio12009; 6:131-136). 展开更多
关键词 left ventricular hypertrophy blood pressure angiotensin converting enzyme genetic polymorphism
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Distribution of Endothelin—Converting Enzyme in the Heart of Human and Rat
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作者 盛红专 朱健华 +4 位作者 吴晓晖 苏恩本 徐东杰 徐晋丹 张寄南 《Journal of Nanjing Medical University》 2003年第2期53-57,共5页
Objective -To determine the distribution of endothelin-converting enzyme (ECE) in different parts of the heart of adult normal Chinese and Sprague-Dawley (SD-) rat. Methods-The ECE mRNA was determined by semi-quantita... Objective -To determine the distribution of endothelin-converting enzyme (ECE) in different parts of the heart of adult normal Chinese and Sprague-Dawley (SD-) rat. Methods-The ECE mRNA was determined by semi-quantitative reverse transcription-polymer ase chain reaction. ECE activity was determined by the conversion of exogenously added big endothelin-1 to endothelin-1. Results : The ECE mRNA and activity were detected in different parts of the heart. In human, the ECE expression in atria was significantly higher than those in ventricles. No significant difference was observed between left and right atrium, and among the left ventricle, right ventricle and interventricular septum. The distribution of ECE activity was consistent with that of mRNA expression. The rat had a similar pattern of distribution of either ECE mRNA expression or activity with that of human. Conclusion :ECE exists in all parts of the heart of normal Chinese and SD rat and its distribution is similar in human and rat hearts. 展开更多
关键词 endothelin-converting enzyme HEART HUMAN RAT
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Nursing care in old patients with heart failure: current status and future perspectives
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作者 Hong-Ying PI Xin HU 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2016年第5期387-390,共4页
The prognosis of heart failure (HF) patients has significantly improved in the last 20 years, given the advent of therapies such as angiotensin-converting enzyme (ACE) inhibitors/angiotensin receptor blockers (A... The prognosis of heart failure (HF) patients has significantly improved in the last 20 years, given the advent of therapies such as angiotensin-converting enzyme (ACE) inhibitors/angiotensin receptor blockers (ARBs), beta-block- ers, and implantable cardioverter-defibrillator (ICD)/cardiac resynchronization therapy devices (CRT). In addition to these promising therapies, proper nursing-care is also important. Here we summarize recent progress of nursing-care strategies in older HF patients, including routine nursing care, transitional care model, self-care, and role of exercise training in old patients with HF. 展开更多
关键词 Heart failure Nursing care Tai Chi The elderly
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Effect of captopril or verapamil on intracellular sodium in cultured vascular smooth muscle cells
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作者 QI JIAN HUA LU ZHANG +3 位作者 JUN WANG MIN LU XIN MING WANG ZHENG JUN JIN(Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.)(Department of Pharmacology, Shanghai Second Medical University, Shanghai 200025, China.) 《Cell Research》 SCIE CAS CSCD 1996年第1期47-53,共7页
The effects of captopril (Cap) and verapamil (Ver)alone and in combination on intracellular Na+ concentration ([Na+]i) in cultured aortic smooth muscle cells (ASMC) of rabbits was evaluated by a direct measurement of ... The effects of captopril (Cap) and verapamil (Ver)alone and in combination on intracellular Na+ concentration ([Na+]i) in cultured aortic smooth muscle cells (ASMC) of rabbits was evaluated by a direct measurement of [Na+]i with fluorescent dye sodium-binding benzofuran isophthalate (SBFI) combined with digital image. [Na+]i in resting cells was found to be 11.9 ± 0. 7 mmol/L. Angiotensin II (Ang-II, 0.1-10μmol/L) induced an increase of [Na+]i in concentration-dependent manner. Ver (0.1-10μmol/L) inhibited Ang-II (1 μmol/L)-induced increase in [Na+]i, while Cap enhanced Ang-II-induced increase in [Na+]i at 10μmol/L but not at 0.1-1μmol/L. Ver (0.1-1μmol/L)abolished enhancement of Ang-II-induced increase in [Na+]i by Cap. Thus, the inhibition of Capenhanced [Na+]i by Ver may provide a new hypothesis for the underlying molecular mechanism of synergistic effect of the combination of Ca2+ antagonists and angiotensinconverting enzyme inhibitors in controlling blood pressure. 展开更多
关键词 Cultured aortic smooth muscle cells angiotensin CAPTOPRIL VERAPAMIL sodiumbinding benzofuran isophthalate
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A population-based case-crossover study of polyethylene glycol use and acute renal failure risk in the elderly
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作者 Nam-Kyong Choi Yoosoo Chang +5 位作者 Sun-Young Jung Yu-Kyong Choi Joongyub Lee Jin-Ho Lee Ju-Young Kim Byung-Joo Park 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第5期651-656,共6页
AIM:To evaluate the possibility of an association between polyethylene glycol(PEG) and acute renal failure(ARF) in elderly patients using a health insurance claims database.METHODS:We conducted a population-based case... AIM:To evaluate the possibility of an association between polyethylene glycol(PEG) and acute renal failure(ARF) in elderly patients using a health insurance claims database.METHODS:We conducted a population-based casecrossover study using information obtained from Korean Health Insurance Review and Assessment Service(HIRA) claims from January 1,2005 to December 31,2005(Seoul,Korea).The study population consisted of elderly patients who received PEG prior to experiencing their first ARF-related hospitalization from April 1,2005 to December 31,2005.For each patient,one case and two control periods were matched.PEG use in a 2-or 4-wk window period prior to hospitalization for ARF was compared with PEG use in two earlier 2-or 4-wk control window periods.Conditional logistic regression analysis was used to estimate odds ratios(ORs) and 95% CI,adjusting for concomitant uses of diuretics,angiotensin converting enzyme inhibitors,non-steroidal anti-inflammatory drugs,antibiotics,anti-cancer drugs,and contrast media.RESULTS:Within the HIRA database which contained 1 093 262 elderly patients,1156 hospitalized ARF cases were identified.Among these cases,PEG was prescribed to 17(1.5%) patients before hospitalization.The adjusted ORs when applying the 2-and 4-wk window periods were 0.4(95% CI:0.03-5.24) and 2.1(95% CI:0.16-27.78),respectively.CONCLUSION:No increased risk of ARF was found in elderly PEG users.However,based on the limited number of study subjects,further analysis should be performed to confirm these results. 展开更多
关键词 Polyethylene glycol Acute renal failure Adverse drug reaction Health insurance claims data-base CASE-CROSSOVER
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The effects of acetaminophen combined with radiation on the radiosensitivity of irradiated human glioma cell progeny
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作者 Li Li Chao Li +4 位作者 Xiaoting Xu Zhiying Yu Songbing Qin Changshao Xu Juying Zhou 《The Chinese-German Journal of Clinical Oncology》 CAS 2009年第4期203-206,共4页
Objective: To study the effects of acetaminophen (ACE) combined with radiation on the progeny of the human glioma cell line SHG-44, and to investigate if ACE may be an useful therapeutic radiosensitivity agent in t... Objective: To study the effects of acetaminophen (ACE) combined with radiation on the progeny of the human glioma cell line SHG-44, and to investigate if ACE may be an useful therapeutic radiosensitivity agent in the treatment of recurrent human glioma. Methods: A randomized, controlled experiment, was performed at the Department of Radiology Laboratory, the First Hospital Affiliated to Soochow University, between September 2004 and January 2006. Brain glioma SHG-44 cells were divided into three groups: SHG-44, SHG-44-10, and SHG-44-10 + ACE cells groups. The SHG-44-10 cells group was irradiated with dose of 10 Gy by a linear accelerator (6 MVX). It was passaged for 15 generations and cultured in RPMI-1640 culture media. Then SHG-44-10 + ACE cells group was treated with ACE. Measures: Community re-double time, mean lethal dose (DO), extrapolation number (N), fraction surviving fraction irradiated by 2 Gy dose (SF2), quasi-threshold dose (Dq), and cell cycle. Results: The SF2 of the SHG-44, SHG-44-10, and SHG-44-10 + ACE cells groups were 70.8%, 80.6% and 45.2%, respectively, with significance (P = 0.040). The SHG-44-10 and SHG-44-10 + ACE cells groups were irradiated with 8 Gy. After 12 hours, the G2/M ratio of the SHG-44-10 and SHG-44-10 + ACE cells groups were indicating significantly higher ratio compared to pre-irradiated groups (P 〈 0.01). After 24 hours, the G2/M ratio of the SHG-44-10 cells group decreased rapidly, while the ratio of the SHG-44-10 + ACE cells group still maintained in high level. Conclusion: In the present study, Subtoxic dose of ACE increased the radiosensitivity of the progeny of irradiated human glioma cell. ACE may be an useful radiosensitivity agent in the treatment of recrudescent human malignant glioma. 展开更多
关键词 glioma cell line SHG44 irradiation acetaminophen (ACE) progenitor cell RADIOSENSITIVITY cell cycle
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CLINICAL OUTCOMES OF FIVE-YEAR FOLLOW-UP OF EARLY AND LONG-TERM TREATMENT WITH CAPTOPRIL ON THE PATIENTS WITH ACUTE MYOCARDIAL INFARCTION
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作者 蔡煦 苏静英 +1 位作者 沈卫峰 龚兰生 《Journal of Shanghai Second Medical University(Foreign Language Edition)》 2002年第1期7-10,29,共5页
Objective To investigate clinical outcomes of early and long-term treatment with captopril on patients with acute myocardial infarction (AMI) during a five-year follow-up. Methods In a randomi- zed trial, 822 patients... Objective To investigate clinical outcomes of early and long-term treatment with captopril on patients with acute myocardial infarction (AMI) during a five-year follow-up. Methods In a randomi- zed trial, 822 patients (623 males, 199 females) with a first AMI with less 72h of symptoms were treated with captopril (treatment group, n=478, dosage from a first 6.25mg to 25mg t.i.d ) and conventional treatment (control group, n=344). Multivariable Cox’ regression were used to analyze relative risk of independent variables. Cumulative survival of both groups were calculated with Kaplan-Meier analysis and analyzed by using log-rank comparison. Results During the five-year follow-up, the age, Killip class (≥Ⅱ), anterior infarction, diabetes mellitus, and peak CPK increased relative risk of death after AML, but the effects of captopril, beta-blocker, antiplatelet drug, and thrombolytic therapy on the relative risk of death were contrary. The cumulative survival in different time during follow-up was higher in patients with captopril than controls (P< 0.001). Conclusion Early and long-term treatment with captopril was related to a beneficial outcome during the five-year follow-up after AMI. 展开更多
关键词 myocardial infarction angiotensin-converting enzyme inhibitor prognosis
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Effect of transforming growth factor beta and bone morphogenetic proteins on rat hepatic stellate cell proliferation and transdifferentiation 被引量:17
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作者 Hong Shen Guo-Jiang Huang Yue-Wen Gong Departments of Internal Medicine,Biochemistry and Medical Genetics,Faculty of Medicine,University of Manitoba,Winnipeg,Manitoba,Canada 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第4期784-787,共4页
AIM: To explore different roles of TGF-β (transforming growth factor beta) and bone morphogenetic proteins (BMPs)in hepatic stellate cell proliferation and trans-differentiation.METHODS: Hepatic stellate cells were i... AIM: To explore different roles of TGF-β (transforming growth factor beta) and bone morphogenetic proteins (BMPs)in hepatic stellate cell proliferation and trans-differentiation.METHODS: Hepatic stellate cells were isolated from male Sprague-Dawley rats. Sub-cultured hepatic stellate cells were employed for cell proliferation assay with WST-1 reagent and Western blot analysis with antibody against smooth muscle alpha actin (SMA).RESULTS: The results indicated that TGF-β1 significantly inhibited cell proliferation at concentration as low as 0.1 ng/ml, but both BMP-2 and BMP-4 did not affect cell proliferation at concentration as high as 10 ng/ml. The effect on hepatic stellate cell trans-differentiation was similar between TGFβ1 and BMPs. However, BMPs was more potent at transdifferentiation of hepatic stellate cells than TGF-β1. In addition, we observed that TGF-β1 transient reduced the abundance of SMA in hepatic stellate cells.CONCLUSION: TGF-β may be more important in regulation of hepatic stellate cell proliferation while BMPs may be the major cytokines regulating hepatic stellate cell transdifferentiation. 展开更多
关键词 ANIMALS Bone Morphogenetic Proteins Cell Differentiation Cell Division Cells Cultured Liver Male RATS Rats Sprague-Dawley Research Support Non-U.S. Gov't Transforming Growth Factor beta
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STUDY ON IMMOBILIZED PORCINE PANCREATIC LIPASE CATALYZING TRANSESTERIFICATION BETWEEN METHYL-BUTYRATE AND 1-BUTANOL IN NONAQUEOUS SYSTEMS
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作者 Xie Zhidong Lu Xianyu +1 位作者 Bao Fengwei He Binglin 《Chinese Journal of Reactive Polymers》 1996年第1期12-18,共7页
Transesterification between methyl-butyrate and 1-butanol in nonaqueous systems was catalyzed by porcine pancreatic lipase which was immobilized on cross- linked polystyrene. Organic solvents, substrate concentration,... Transesterification between methyl-butyrate and 1-butanol in nonaqueous systems was catalyzed by porcine pancreatic lipase which was immobilized on cross- linked polystyrene. Organic solvents, substrate concentration, contents of water and other parameters which affect the immobilized enzyme activity were studied. Lipase immobilized on hydrophobic crosslinked polystyrene can reduce its diffusion limit in the reaction. It was found that the activity of immobilized lipase in organic systems was two times as high as that of free lipase. 展开更多
关键词 Porcine Pancreatic Lipase TRANSESTERIFICATION Immobilized Enzyme Nonaqueous Systems
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