The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distributi...The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distribution patterns of MTS were observed in the different growth zones of root tips. MTS of the cortical cells were randomly aligned in the zone of cell division. They were transversely arranged in the cortical cells of the zone of cell elongation; and obliquely oriented in the root hair zone. After treatment with 2,4_D, MTS displayed distinct changes with reorientation in the cortex of the root tip coupling with the inhibition of root growth. MTS changed their orientation in the cortical cells of the zone of cell division from being randomly oriented to transversely oriented when incubated in 1 mg/L 2,4_D for 1 h. However, they were recovered and became randomly oriented when the roots were treated with 1 mg/L 2,4_D for 24 h. An array of MTS, which was different from that in the control, was observed in the cortical cells of the zone of cell elongation in the roots treated with 1 mg/L 2,4_D for 1 h. After treatment with 10 mg/L 2,4_D for 1 h, the MTS in the cortical cells of the zone of cell division became transversely reorientated, but the pattern of MTS distribution was different from that in the 1_hour treatment of 1 mg/L 2,4_D. MTS were hardly detected in the same type of cells when roots were submerged in 10 mg/L 2,4_D for 24 h. When roots were incubated in 10 mg/L 2,4_D for 1 h, the MTS of the cortical cells in the zone of cell elongation became randomly oriented, and much more randomly when the roots were treated for 24 h.展开更多
文摘The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distribution patterns of MTS were observed in the different growth zones of root tips. MTS of the cortical cells were randomly aligned in the zone of cell division. They were transversely arranged in the cortical cells of the zone of cell elongation; and obliquely oriented in the root hair zone. After treatment with 2,4_D, MTS displayed distinct changes with reorientation in the cortex of the root tip coupling with the inhibition of root growth. MTS changed their orientation in the cortical cells of the zone of cell division from being randomly oriented to transversely oriented when incubated in 1 mg/L 2,4_D for 1 h. However, they were recovered and became randomly oriented when the roots were treated with 1 mg/L 2,4_D for 24 h. An array of MTS, which was different from that in the control, was observed in the cortical cells of the zone of cell elongation in the roots treated with 1 mg/L 2,4_D for 1 h. After treatment with 10 mg/L 2,4_D for 1 h, the MTS in the cortical cells of the zone of cell division became transversely reorientated, but the pattern of MTS distribution was different from that in the 1_hour treatment of 1 mg/L 2,4_D. MTS were hardly detected in the same type of cells when roots were submerged in 10 mg/L 2,4_D for 24 h. When roots were incubated in 10 mg/L 2,4_D for 1 h, the MTS of the cortical cells in the zone of cell elongation became randomly oriented, and much more randomly when the roots were treated for 24 h.
