The purpose of this paper is to implement ＂Soil and Water Assessment Tool （SWAT）＂ model to examine the effects of land use change scenarios; associated with crop rotations and special cultivation techniques most s...The purpose of this paper is to implement ＂Soil and Water Assessment Tool （SWAT）＂ model to examine the effects of land use change scenarios; associated with crop rotations and special cultivation techniques most susceptible to erosion; exert on runoff discharge and sediment yield from Song Cau catchment in Northern Viet Nam. All scenarios＇ simulations resulted in a decrease of soil losses and sediment yield comparing to the current land use status. SWAT successfully predicted soil losses from different HRUs that caused significant sediment yield, and it predicted explicitly the consequences of non-structural mitigation measures against erosion.展开更多
Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order t...Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4+ CD25+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4+ CD25+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4+ CD25+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4+ CD25+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4+ CD25+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.展开更多
文摘The purpose of this paper is to implement ＂Soil and Water Assessment Tool （SWAT）＂ model to examine the effects of land use change scenarios; associated with crop rotations and special cultivation techniques most susceptible to erosion; exert on runoff discharge and sediment yield from Song Cau catchment in Northern Viet Nam. All scenarios＇ simulations resulted in a decrease of soil losses and sediment yield comparing to the current land use status. SWAT successfully predicted soil losses from different HRUs that caused significant sediment yield, and it predicted explicitly the consequences of non-structural mitigation measures against erosion.
基金Project supported by the National Natural Science Foundation of China (Nos. 30872578 and 30753761)the Natural Science Founda-tion of Shanxi Province (No. SJ08C201)+1 种基金the Science and Technology Key Projects Foundation of Shanxi Province (No. 2008K13-04)the Science and Technology Plan Projects Foundation of Xi’an (No. SF08006-2), China
文摘Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4+ CD25+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4+ CD25+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4+ CD25+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4+ CD25+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4+ CD25+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.
