Recently,the use of confocal laser endomicroscopy(CLE) in the diagnosis of chronic ulcerative colitis(CUC) was reported.In this brief report we aimed to assess the application of probe-based CLE to characterize coloni...Recently,the use of confocal laser endomicroscopy(CLE) in the diagnosis of chronic ulcerative colitis(CUC) was reported.In this brief report we aimed to assess the application of probe-based CLE to characterize colonic mucosa and dysplasia in CUC.The study involved a patient presenting long-standing CUC.Confocal imaging of both the inflamed mucosa,a circumscribed lesion(dysplasiaassociated lesional mass),and adjacent colonic mucosa are demonstrated and the correlation between the CLE and histological images.Inflamed mucosa and dysplasia showed specific alteration of crypt architecture,cellular infiltration,and vessel architecture with an excellent correlation between CLE and standard histological examination.展开更多
A fast and facile method of fabricating fiber-optic localized surface plasmon resonance sensors baseff on spherical gold nanoparticles was introduced in this study. The gold nanoparticles with an average diameter of 5...A fast and facile method of fabricating fiber-optic localized surface plasmon resonance sensors baseff on spherical gold nanoparticles was introduced in this study. The gold nanoparticles with an average diameter of 55 nm were synthesized via the Turkevich method and were then immobilized onto the surface of an uncladded sensor probe using a polydopamine layer. To obtain a sensor probe with high sensitivity to changes in the refractive index, a set of key optimization parameters, including the sensing length, coating time of the potydopamine layer, and coating time of the gold nanoparticles, were investigated. The sensitivity of the optimized sensor probe was 522.80 nm per refractive index unit, and the probe showed distinctive wavelength shifts when the refractive index was changed from 1.328 6 to 1.398 7. When stored in deionized water at 4 ℃, the sensor probe proved to be stable over a period of two weeks. The sensor also exhibited advantages, such as low cost, fast fabrication, and simple optical setup, which indicated its potential application in remote sensing and real-time detection.展开更多
In the absence of a simple technique to predict convection heat transfer on BIPV （building integrated photovoltaic） surfaces, a mobile probe with two thermocouples was designed. Thermal boundary layers on vertical f...In the absence of a simple technique to predict convection heat transfer on BIPV （building integrated photovoltaic） surfaces, a mobile probe with two thermocouples was designed. Thermal boundary layers on vertical flat surfaces ofa PV （photovoltaic） and a metallic plate were traversed. The plate consisted of twelve heaters where heat flux and surface temperature were controlled and measured. Uniform heat flux condition was developed on the heaters to closely simulate non-uniform temperature distribution on vertical PV modules. The two thermocouples on the probe measured local air temperature and contact temperature with the wall surface. Experimental results were presented in the forms of local Nusselt numbers versus Rayleigh numbers ＂Nu = a＇（Ra）b＇＇, and surface temperature versus dimensionless height （Ts - T∞ = c.（z/h）d）. The constant values for ＂a＂, ＂b＂, ＂c＂ and ＂d＂ were determined from the best curve-fitting to the power-law relation. The convection heat transfer predictions from the empirical correlations were found to be in consistent with those predictions made by a number of correlations published in the open literature. A simple technique is then proposed to employ two experimental data from the probe to refine empirical correlations as the operational conditions change. A flexible technique to update correlations is of prime significance requirement in thermal design and operation of BIPV modules. The work is in progress to further extend the correlation to predict the combined radiation and convection on inclined PVs and channels.展开更多
This paper presents a new design of a probe adjusting device intended to position pressure and temperature probes in a flow field. 5-hole, 3-hole and temperature probes can be moved in radial direction and freely rota...This paper presents a new design of a probe adjusting device intended to position pressure and temperature probes in a flow field. 5-hole, 3-hole and temperature probes can be moved in radial direction and freely rotated about their axis. The high actuation accuracy of 3.9 ktm in radial direction and 0.09~ in angular position is validated in a 2-stage-turbine test rig which is installed at the Institute of Power Plant Technology, Steam and Gas Turbines, RWTH Aachen University. To meet the challenge to calculate the efficiency of a turbo machine which is mainly influenced by the temperature, all probe adjusting devices are positioned simultaneously and controlled by the MAS （measuring acquisition system） so that the same radial position in each stage is measured at the same time. For this purpose a new program has been developed to synchronize actuation and measurement. The slim design of 60 mm width allows measurement between the stages of turbo machines with small axial distances between vane and blade. In addition a CFD/FEA shows how the design and combination of materials compensate the thermal expansion of the engine during operation. This allows a minimal safety distance of 0.2 mm between rotor and probe to enable measurement as close to the physical boundary as possible. The actuation accuracy is demonstrated with pressure, temperature and angle distribution plots. It is also shown that the resolution of the measuring points, and therefore the actuation distances, has a large impact on the flow field analysis and should be set as high as possible. However the measuring time has to be taken into account.展开更多
Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on...Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently at- tracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quanti- fied the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5×10^-3 to 4.7×10^-2 pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k≥3.0×10^-2 pN/nm. This work furthered the understanding of the forced dissociation of se- lectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukoeytes under blood flow.展开更多
文摘Recently,the use of confocal laser endomicroscopy(CLE) in the diagnosis of chronic ulcerative colitis(CUC) was reported.In this brief report we aimed to assess the application of probe-based CLE to characterize colonic mucosa and dysplasia in CUC.The study involved a patient presenting long-standing CUC.Confocal imaging of both the inflamed mucosa,a circumscribed lesion(dysplasiaassociated lesional mass),and adjacent colonic mucosa are demonstrated and the correlation between the CLE and histological images.Inflamed mucosa and dysplasia showed specific alteration of crypt architecture,cellular infiltration,and vessel architecture with an excellent correlation between CLE and standard histological examination.
基金Supported by the Ministry of Science and Technology of China(No.2012YQ090194)the National Natural Science Foundation of China(No.51473115)
文摘A fast and facile method of fabricating fiber-optic localized surface plasmon resonance sensors baseff on spherical gold nanoparticles was introduced in this study. The gold nanoparticles with an average diameter of 55 nm were synthesized via the Turkevich method and were then immobilized onto the surface of an uncladded sensor probe using a polydopamine layer. To obtain a sensor probe with high sensitivity to changes in the refractive index, a set of key optimization parameters, including the sensing length, coating time of the potydopamine layer, and coating time of the gold nanoparticles, were investigated. The sensitivity of the optimized sensor probe was 522.80 nm per refractive index unit, and the probe showed distinctive wavelength shifts when the refractive index was changed from 1.328 6 to 1.398 7. When stored in deionized water at 4 ℃, the sensor probe proved to be stable over a period of two weeks. The sensor also exhibited advantages, such as low cost, fast fabrication, and simple optical setup, which indicated its potential application in remote sensing and real-time detection.
文摘In the absence of a simple technique to predict convection heat transfer on BIPV （building integrated photovoltaic） surfaces, a mobile probe with two thermocouples was designed. Thermal boundary layers on vertical flat surfaces ofa PV （photovoltaic） and a metallic plate were traversed. The plate consisted of twelve heaters where heat flux and surface temperature were controlled and measured. Uniform heat flux condition was developed on the heaters to closely simulate non-uniform temperature distribution on vertical PV modules. The two thermocouples on the probe measured local air temperature and contact temperature with the wall surface. Experimental results were presented in the forms of local Nusselt numbers versus Rayleigh numbers ＂Nu = a＇（Ra）b＇＇, and surface temperature versus dimensionless height （Ts - T∞ = c.（z/h）d）. The constant values for ＂a＂, ＂b＂, ＂c＂ and ＂d＂ were determined from the best curve-fitting to the power-law relation. The convection heat transfer predictions from the empirical correlations were found to be in consistent with those predictions made by a number of correlations published in the open literature. A simple technique is then proposed to employ two experimental data from the probe to refine empirical correlations as the operational conditions change. A flexible technique to update correlations is of prime significance requirement in thermal design and operation of BIPV modules. The work is in progress to further extend the correlation to predict the combined radiation and convection on inclined PVs and channels.
文摘This paper presents a new design of a probe adjusting device intended to position pressure and temperature probes in a flow field. 5-hole, 3-hole and temperature probes can be moved in radial direction and freely rotated about their axis. The high actuation accuracy of 3.9 ktm in radial direction and 0.09~ in angular position is validated in a 2-stage-turbine test rig which is installed at the Institute of Power Plant Technology, Steam and Gas Turbines, RWTH Aachen University. To meet the challenge to calculate the efficiency of a turbo machine which is mainly influenced by the temperature, all probe adjusting devices are positioned simultaneously and controlled by the MAS （measuring acquisition system） so that the same radial position in each stage is measured at the same time. For this purpose a new program has been developed to synchronize actuation and measurement. The slim design of 60 mm width allows measurement between the stages of turbo machines with small axial distances between vane and blade. In addition a CFD/FEA shows how the design and combination of materials compensate the thermal expansion of the engine during operation. This allows a minimal safety distance of 0.2 mm between rotor and probe to enable measurement as close to the physical boundary as possible. The actuation accuracy is demonstrated with pressure, temperature and angle distribution plots. It is also shown that the resolution of the measuring points, and therefore the actuation distances, has a large impact on the flow field analysis and should be set as high as possible. However the measuring time has to be taken into account.
基金supported by the National Natural Science Foundation of China (Grant Nos. 10902117, 10702075, 30730032 and 11072251)Chinese Academy of Sciences (Grant Nos. KJCX2-YW-L08 and Y2010030)+1 种基金the National Key Basic Research Foundation of China (Grant No. 2011CB710904)Dr. MCEVER Rodger P. (Oklahoma Medical Research Foundation) for generous gifts of P-selectin, S12, G1, PL2, and PL1 proteins
文摘Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently at- tracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quanti- fied the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5×10^-3 to 4.7×10^-2 pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k≥3.0×10^-2 pN/nm. This work furthered the understanding of the forced dissociation of se- lectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukoeytes under blood flow.
