OBJECTIVE: To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease (ALD). METHODS: Sixty-five healthy male controls and 165 alcoholis...OBJECTIVE: To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease (ALD). METHODS: Sixty-five healthy male controls and 165 alcoholisms (including 122 ALD patients and 43 male alcohol abusers without liver complications defined as alcohol-dependent) were analyzed by polymerase chain reaction and hybridized with oligonucleotide microarray to detect the polymorphisms of the ethanol metabolizing enzymes genes. RESULTS: The frequencies of alcohol dehydrogenase gene 2 * 1 ( ADH2 * 1 ) allele were shown as 37.69%, 46.51% and 59.02% in control, alcohol-dependent and ALD groups respectively; while those of ADH2 * 2 allele were shown as 62.31 %, 53.49% and 40.98% respectively. No ADH2 * 3 was detected in any of the subjects. The frequency of ADH2 * 1 was significantly higher in alcoholisms (ALD group and alcohol-dependent group) than in healthy controls ( P展开更多
A novel linear microprobe array(LMPA)has been developed by a conventional microfabrication method from silicon.The LMPA leverages the properties of conventional microwire with additional features of naturally formed r...A novel linear microprobe array(LMPA)has been developed by a conventional microfabrication method from silicon.The LMPA leverages the properties of conventional microwire with additional features of naturally formed regular spacing.With the help of periodic microprobe arrays and double-side V-grooves fabricated in advance between each pair of the two microprobes’rear ends,the number of microprobe units for assembly in one array can be flexibly chosen by cleavage fracture from the LMPA.The fabrication method was demonstrated and the prototype device was assessed by electrochemical impedance spectroscopy(EIS)and in vivo test.The SNR of the spikes recorded was 6.展开更多
文摘OBJECTIVE: To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease (ALD). METHODS: Sixty-five healthy male controls and 165 alcoholisms (including 122 ALD patients and 43 male alcohol abusers without liver complications defined as alcohol-dependent) were analyzed by polymerase chain reaction and hybridized with oligonucleotide microarray to detect the polymorphisms of the ethanol metabolizing enzymes genes. RESULTS: The frequencies of alcohol dehydrogenase gene 2 * 1 ( ADH2 * 1 ) allele were shown as 37.69%, 46.51% and 59.02% in control, alcohol-dependent and ALD groups respectively; while those of ADH2 * 2 allele were shown as 62.31 %, 53.49% and 40.98% respectively. No ADH2 * 3 was detected in any of the subjects. The frequency of ADH2 * 1 was significantly higher in alcoholisms (ALD group and alcohol-dependent group) than in healthy controls ( P
基金supported the National Basic Research Program of China("973"Project)(Grant Nos.2011CB933203 and 2011CB933102)the National Hi-Tech Research and Development Program of China("863"Project)(Grant Nos.2012AA030308 and 2013AA032204)+1 种基金the National Natural Science Foundation of China(Grant Nos.61275200,61335010,61178051 and 61178082)the National Important Scientific Apparatus Developing Project(Grant No.2011YQ04008204)
文摘A novel linear microprobe array(LMPA)has been developed by a conventional microfabrication method from silicon.The LMPA leverages the properties of conventional microwire with additional features of naturally formed regular spacing.With the help of periodic microprobe arrays and double-side V-grooves fabricated in advance between each pair of the two microprobes’rear ends,the number of microprobe units for assembly in one array can be flexibly chosen by cleavage fracture from the LMPA.The fabrication method was demonstrated and the prototype device was assessed by electrochemical impedance spectroscopy(EIS)and in vivo test.The SNR of the spikes recorded was 6.
