[Objective] This paper aimed to improve the detection rate of probe EUB338 for eubacteria.[Methods] Sixteen strains were used as materials for the experiment and analyzed through fluorescence in situ hybridization.[Re...[Objective] This paper aimed to improve the detection rate of probe EUB338 for eubacteria.[Methods] Sixteen strains were used as materials for the experiment and analyzed through fluorescence in situ hybridization.[Result] The average detection rate of probe EUB338 was 63.9%,which can be increased to 71.3% through modified FISH.[Conclusion] This research was able to provide scientific basis for the detection of eubacteria in soil from the perspective of molecular biology.展开更多
基金Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministrythe National Natural Science Foundation of China(No.21047001)the Standards Foundation of Shanghai City(No.10DZ0503900)~~
文摘[Objective] This paper aimed to improve the detection rate of probe EUB338 for eubacteria.[Methods] Sixteen strains were used as materials for the experiment and analyzed through fluorescence in situ hybridization.[Result] The average detection rate of probe EUB338 was 63.9%,which can be increased to 71.3% through modified FISH.[Conclusion] This research was able to provide scientific basis for the detection of eubacteria in soil from the perspective of molecular biology.
