针对卫星通信工作频率越来越高的应用需求,设计了一种工作在极高频(Extremely High Frequency,EHF)的接收模块,实现极高频射频信号的接收及下变频功能。模块内部集成波导-微带探针过渡、低噪声放大器、微带滤波器、本振电路、混频器及...针对卫星通信工作频率越来越高的应用需求,设计了一种工作在极高频(Extremely High Frequency,EHF)的接收模块,实现极高频射频信号的接收及下变频功能。模块内部集成波导-微带探针过渡、低噪声放大器、微带滤波器、本振电路、混频器及供电电路,具有低噪声系数、高增益、高带外抑制的优点。采用单片微波集成电路(Monolithic Microwave Integrated Circuit,MMIC)混合多功能集成技术实现了模块的小型化、通用化,可适应多种场景下的极高频收发前端应用,具有广阔的应用前景。展开更多
Time-resolved (TR)photoluminescence (PL) technique has shown great promise in ultrasensitive biodetection and high-resolution bioimaging.Hitherto,almost all the TRPL bioprobes are based on the parity-forbidden f→f tr...Time-resolved (TR)photoluminescence (PL) technique has shown great promise in ultrasensitive biodetection and high-resolution bioimaging.Hitherto,almost all the TRPL bioprobes are based on the parity-forbidden f→f transition of lanthanide ions.Herein,we report TRPL biosensing by taking advantage of the d→d transition of transition metal (TM)Mn^2+ ion.We demonstrate that the Forster resonance energy transfer (FRET)signal can be distinguished from that of radiative reabsorption process through measuring the PL lifetime of Mn^2+,thus establishing a reliable method for Mn^2+ in homogeneous TR-FRET biodetection.We also demonstrate the biotin receptor-targeted cancer cell imaging by utilizing biotinylated CaF2:Ce,Mn nanoprobes.Furthermore,we show in a proof-of-concept experiment the appli- cation of the long-lived PL of Mn^2+ for TRPL bioimaging through the burst shot with a cell phone.These findings provide a general approach for exploiting the long-lived PL of TM ions for TRPL biosensing,thereby opening up a new avenue for the exploration of novel and versatile applications of TM ions.展开更多
基金supported by National Program on Key Basic Research Project (973 Program, 2014CB845605) the Strategic Priority Research Program of the CAS (XDB20000000)+3 种基金 the National Natural Science Foundation of China (21325104, 11774345, 21771185, 21501180 and 21650110462)the CAS/SAFEA International Partnership Program for Creative Re-search Teams, the Youth Innovation Promotion Association (2016277)the Chunmiao Project of Haixi Institutes of the CAS (CMZX-2016-002)Natural Science Foundation of Fujian Province (2017I0018 and 2017J05095)
文摘Time-resolved (TR)photoluminescence (PL) technique has shown great promise in ultrasensitive biodetection and high-resolution bioimaging.Hitherto,almost all the TRPL bioprobes are based on the parity-forbidden f→f transition of lanthanide ions.Herein,we report TRPL biosensing by taking advantage of the d→d transition of transition metal (TM)Mn^2+ ion.We demonstrate that the Forster resonance energy transfer (FRET)signal can be distinguished from that of radiative reabsorption process through measuring the PL lifetime of Mn^2+,thus establishing a reliable method for Mn^2+ in homogeneous TR-FRET biodetection.We also demonstrate the biotin receptor-targeted cancer cell imaging by utilizing biotinylated CaF2:Ce,Mn nanoprobes.Furthermore,we show in a proof-of-concept experiment the appli- cation of the long-lived PL of Mn^2+ for TRPL bioimaging through the burst shot with a cell phone.These findings provide a general approach for exploiting the long-lived PL of TM ions for TRPL biosensing,thereby opening up a new avenue for the exploration of novel and versatile applications of TM ions.
