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稀释接种法和二次发酵法制备的绿色木霉生物有机肥肥料效应比较研究
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作者 邓杰 王宇蕴 +3 位作者 唐伟 魏雨泉 李季 徐智 《中国土壤与肥料》 CAS CSCD 北大核心 2024年第5期162-168,共7页
生物有机肥中的功能性微生物是生物有机肥肥效的核心,生物有机肥的制造工艺决定了功能性微生物的数量和应用效果,绿色木霉兼具的促生和生防功能现已被广泛应用于生物有机肥的制备中。分别采用稀释接种法和二次发酵法在不同腐熟度(种子... 生物有机肥中的功能性微生物是生物有机肥肥效的核心,生物有机肥的制造工艺决定了功能性微生物的数量和应用效果,绿色木霉兼具的促生和生防功能现已被广泛应用于生物有机肥的制备中。分别采用稀释接种法和二次发酵法在不同腐熟度(种子发芽指数GI值为50%、80%、100%)的堆肥物料接种绿色木霉制备生物有机肥,采用盆栽试验,研究两种工艺类型的生物有机肥在辣椒根系定殖能力及其对辣椒疫病的防控作用。结果发现,施用生物有机肥24 d后,经过二次发酵法生产的生物有机肥(分别在堆肥物料GI值为50%、80%和100%时接种绿色木霉进行二次发酵生产的生物有机肥)的绿色木霉在辣椒植株根系定殖量分别为1.5×10^(5)、2.3×10^(5)和1.02×10^(5)CFU/g,按照稀释接种法生产的生物有机肥(分别在堆肥物料GI值为50%、80%和100%时,按照稀释倍数接种绿色木霉生产的生物有机肥)的绿色木霉定殖量分别为0.29×10^(5)、0.72×10^(5)和0.24×10^(5)CFU/g;在GI值为80%的堆肥物料条件接种绿色木霉,经过二次发酵生产的生物有机肥,绿色木霉在辣椒根际定殖能力最强;经过二次发酵的生物有机肥处理的辣椒疫病防治效果显著高于按照稀释倍数接种绿色木霉制成的生物有机肥处理,且以GI值为80%的堆肥物料条件生产的生物有机肥的辣椒疫病防治效果最好。研究结果发现,以GI值为80%的堆肥物料接种绿色木霉后,采用二次发酵方式生产的生物有机肥,7 d后的绿色木霉菌增殖量为3.91倍,表现出功能性微生物最好的增殖和定殖效果。综上所述,在制备绿色木霉生物有机肥时的最佳方法是在腐熟度为80%的堆肥物料中接种绿色木霉进行二次发酵,可以提高绿色木霉的定殖效果,也可以提升绿色木霉生物有机肥的抗病效果。 展开更多
关键词 不同腐熟度堆肥物料 接种工艺 绿色木霉 定殖能力 防治效果
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果粒酸牛奶配方与制备工艺初步探究
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作者 温宇旸 《中国食品工业》 2021年第21期104-107,共4页
主要探讨果肉添加量,加糖量、发酵时间、复配稳定剂对果粒酸牛奶品质的影响,通过制备工艺的研究,以促进节能环保下酸牛奶更好的发展。
关键词 果粒酸奶 发酵时间 接种 制备工艺
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微载体培养技术的研究进展 被引量:13
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作者 郭燕华 郭勇 罗立新 《生物工程进展》 CAS CSCD 2001年第5期56-58,共3页
本文对近年来广泛用于动物细胞培养的微载体技术的特点、培养过程的优化作了较为详细的评述。
关键词 微载体培养 贴壁依赖型细胞 定量影像分析法 接种工艺 动物细胞
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威海地区适栽香菇品种的选择、液体制种及高效栽培技术 被引量:1
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作者 汪建党 侯艳平 +2 位作者 韩建东 汪潇莉 万鲁长 《山东农业科学》 2014年第8期125-126,131,共3页
介绍了适宜威海地区栽培的香菇品种808、939和雨花2号的生长发育特性、适合制作香菇液体菌种的培养基配方、生产工艺和接种方法,总结出了香菇高效栽培技术。
关键词 香菇 品种选择 液体菌种 接种工艺 栽培技术
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Microstructure of partially remelted billet of AM60 alloy prepared with self-inoculation method 被引量:4
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作者 邢博 李元东 +2 位作者 马颖 陈体军 郝远 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第9期1622-1629,共8页
The billets of AM60 alloy, prepared with self-inoculation method, were partially remelted into semisolid state. Effects of process parameters on remelting microstructure of semisolid billet were investigated. Experime... The billets of AM60 alloy, prepared with self-inoculation method, were partially remelted into semisolid state. Effects of process parameters on remelting microstructure of semisolid billet were investigated. Experimental results show that the solid particles obtained with self-inoculation method are in smaller grain size and globular shape after partial remelting, compared with those prepared with other casting methods. In the optimized process conditions, the average size of solid particles of partially remelted billet is 65 μm, and the shape factor is 1.12. The process parameters, i.e. pouting temperature, addition amount of self-inoculants, and the slope angle of multi-stream mixing cooling chalmel have influence on the microstructure of partially remelted billet. The optimized temperature is from 680 ℃ to 700 ℃, addition amount of self-inoculants is between 5% and 7% (mass fraction), slope angle of multi-stream mixing cooling channel is between 30° and 45°, with which the dendritic microstructure of as-cast billet can be avoided, and the size of solid particles ofremelted billet is reduced. 展开更多
关键词 AM60 Alloy self-inoculation method semisolid process partial remelting process parameters
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Influences of diffusion bonding process parameters on bond characteristics of Mg-Cu dissimilar joints 被引量:11
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作者 G. MAHENDRAN V. BALASUBR AMANIAN T. SENTHILVELAN 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第6期997-1005,共9页
In many circumstances,dissimilar metals have to be bonded together and the resulting joint interfaces must typically sustain mechanical and/or electrical forces without failure,which is not possible by fusion welding ... In many circumstances,dissimilar metals have to be bonded together and the resulting joint interfaces must typically sustain mechanical and/or electrical forces without failure,which is not possible by fusion welding processes.The melting points of magnesium(Mg)and copper(Cu)have a significant difference(nearly 400℃)and this may lead to a large difference in the microstructure and joint performance of Mg-Cu joints.However,diffusion bonding can be used to join these alloys without much difficulty.This work analyses the effect of parameters on diffusion layer thickness,hardness and strength of magnesium-copper dissimilar joints.The experiments were conducted using three-factor,five-level,central composite rotatable design matrix.Empirical relationships were developed to predict diffusion layer thickness,hardness and strength using response surface methodology.It is found that bonding temperature has predominant effect on bond characteristics.Joints fabricated at a bonding temperature of 450℃, bonding pressure of 12 MPa and bonding time of 30 min exhibited maximum shear strength and bonding strength of 66 and 81 MPa, respectively. 展开更多
关键词 diffusion bonding magnesium alloy copper alloy shear strength bonding strength diffusion layer thickness interface hardness
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