MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility...MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility of MTs as a biomarker of Sb pollution. A contact method of filter paper was used to investigate Sb inducing MTs in Eiseniafoetida. Spectrophotometrical and SDS-PAGE （sodium dodecyl sulphate-polyacrylamide gel electropheresis） analyses were used to determine the nature of the induced MTs samples. Rabbit liver metallothioneins was used as a standard. The results showed that the molecular weight of induced protein was approximately 6-7 kDa. A characteristic absorption peak at 268 nm was observed, which was consistent with the standard. The amounts of Sb-MTs quantified by cadmium hemoglobin saturation method showed a significant positive relationship with increasing Sb exposure.The experiments prove that Sb can induce Sb-MTs in Eiseniafoetida as a biomarker of antimony pollution.展开更多
文摘MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility of MTs as a biomarker of Sb pollution. A contact method of filter paper was used to investigate Sb inducing MTs in Eiseniafoetida. Spectrophotometrical and SDS-PAGE （sodium dodecyl sulphate-polyacrylamide gel electropheresis） analyses were used to determine the nature of the induced MTs samples. Rabbit liver metallothioneins was used as a standard. The results showed that the molecular weight of induced protein was approximately 6-7 kDa. A characteristic absorption peak at 268 nm was observed, which was consistent with the standard. The amounts of Sb-MTs quantified by cadmium hemoglobin saturation method showed a significant positive relationship with increasing Sb exposure.The experiments prove that Sb can induce Sb-MTs in Eiseniafoetida as a biomarker of antimony pollution.
