The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome(PCOS),which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS fe...The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome(PCOS),which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS female monkeys.Six adult female rhesus monkeys aged 6-10 a,were divided into a PCOS group and a control group.The PCOS group were given two cycles of subcutaneous injections of propionic acid testosterone(PAT),3.5 mg/kg body weight,on day 1,day 3,and day 5 of the menstrual cycle,respectively,and then given muscle injections of human chorionic gonadotropin(HCG),350 IU/kg body wtight,on day 7,day 9,and day 11,respectively.Results showed that high levels of serum LH and T [(5.35±0.17) IU/L and(7.58±0.14) ng/mL,respectively],and a high ratio value of LH/FSH(5.35/1.30=4.12) were observed in the PCOS group.No significant differences were found in serum FSH,E2,and P in the PCOS group compared with those of the control.Polycystic ovaries in the PCOS monkeys were recorded by live ultrasound.The blastocysts rates of the PCOS vs.the control were 23.53% vs.66.67%,and there was a significant difference between the two groups.This study shows that PAT coupled with HCG can induce PCOS in rhesus monkeys in the short term.The reproductive features of PCOS monkeys were similar to those of PCOS patients.展开更多
Objective:To address how genistein sensitizes the chemotherapy-resistant ovarian carcinoma cells and promotes apoptosis in the respect of cell cycle and the regulation of survivin expression in the process.Methods:Ova...Objective:To address how genistein sensitizes the chemotherapy-resistant ovarian carcinoma cells and promotes apoptosis in the respect of cell cycle and the regulation of survivin expression in the process.Methods:Ovarian SKOV-3 carcinoma cell line was treated with genistein or cisplatin either alone or in combination.Cell viability was showed by MTT method.Cell cycle and apoptosis were detected by flow cytometry.Survivin mRNA and protein were revealed by RT-PCR and immunocytochemistry,respectively.Results:Genistein could reduce the cell viability in a dose-dependent manner,while cisplatin did so at a much higher level.In contrast,if the two agents were treated in combination,half growth inhibition(IC50) value for cisplatin was reduced remarkably and the effect was synergistic as analyzed by isobologram.In particular,the reduced cell viability was exhibited by a switch in cell cycle progression,as the cells were arrested in G2/M phase and the G0/G1 phase-fraction was significantly decreased.The reduced cell viability appeared to involve apoptosis,based on our results from flow cytometry and Hoechst 33258 staining.In the meanwhile,genistein performed the inhibitory effect on cisplatin-induced survivin expression.Conclusion:Genistein can sensitize ovarian carcinoma cells to cisplatin therapy with the inhibition of survivin expression as the potential mechanism.展开更多
Efficacy of the egg parasitoid, Trichogramma evanescens and the bio-pesticide bacteria, Bacillus thuringiensis kurstaki (Btk) in reduction of lesser date moth, Batrachedra amydraula infestations were studied during ...Efficacy of the egg parasitoid, Trichogramma evanescens and the bio-pesticide bacteria, Bacillus thuringiensis kurstaki (Btk) in reduction of lesser date moth, Batrachedra amydraula infestations were studied during seasons of 2011 and 2012. The result of 2011 showed that the release of egg parasitoid by rate of 500 and 1,000 parasitoids/palm tree achieved 55.06% and 67.45% reduction in infestation percentage, respectively. Meanwhile high reduction in infestation was achieved by Btk (78.65%) at dose rate of 6 g Btk powder/kg talc powder. The result of 2012 showed that the highest control efficacy (58.78%) was achieved at the first time of the control process, using Btk at dose rate of 6 g/kg talc powder. This result was not different significantly from the release of egg parasitoid by rate of 1,000 parasitoids/palm tree which achieved 53% reduction in infestation percentage. At the second time (after one week from the first application), the efficacy of Btk at dose rate of 6g/kg talc powder was increased to 78.78%, which differ significantly with the release of egg parasitoid by the same rate of 1,000 parasitoids/palm tree, having 61.79% efficacy. Reapplication of the control method achieved an increase in efficacy of the control agents, such increase was significant for both the release of the parasitoid at rate of 1,000/palm tree and for Btk at a dose of 6 g/kg talc powder.展开更多
In present study, a neogregarine from Crioceris asparagi is reported for the first time in Turkey. C. asparagi (common asparagus beetle) is an important pest of Asparagus officinalis L. crops in Turkey and the world...In present study, a neogregarine from Crioceris asparagi is reported for the first time in Turkey. C. asparagi (common asparagus beetle) is an important pest of Asparagus officinalis L. crops in Turkey and the world. Therefore, determining of the protist pathogens of C. asparagi is aimed. During the study, a total of 1,099 C. asparagi adults were examined thoroughly using a light microscope. Insects were collected from the provinces of Manisa, Eskisehir and Bahkesir in Turkey, with 117 of them collected from Manisa, 204 of them from Bahkesir and 778 of them from Eskisehir. A neogregarine pathogen was found in Bahkesir and Manisa. Total infection rate was 18% for Bahkesir between the years 2014-2015 and 15.3% for Manisa in 2011. Oocysts of the neogregarine pathogen were observed only in malpighian tubes of C. asparagi adults. Fresh mature oocysts of the neogregarine was measured as 9.34 ± 0.74 lam (n = 50) in length and 5.27 ± 0.36 μm (n = 50) in width. Identification of entomopathogens is prerequisite for their use in biological control.展开更多
Objective To observe the effect of electroacupuncture (EA) on egg quality of polycystic ovarian syndrome (PCOS) patients, and to explore its mechanism. Methods Two hundred patients who received in vitro fertilizat...Objective To observe the effect of electroacupuncture (EA) on egg quality of polycystic ovarian syndrome (PCOS) patients, and to explore its mechanism. Methods Two hundred patients who received in vitro fertilization-embryo transfer (IVF-ET) were divided into an EA group (102 cases) and a control group (98 cases) according to random number table. All the patients in the two groups were given Diane-35 and gonadotropin-releasing hormone agonist (GnRH-α) for ovarian hyperstimulation. Besides, EA intervention was applied to Shenshu (肾俞 BL 23), Qihai (气海 GV 6), Zusanli (足三里ST 36), Sanyinjiao (三阴交 SP 6), Neiguan (内关 PC 6) and Zigong (子宫 EX-CA1), etc. in the EA group. Then egg quality, final outcome of pregnancy and levels of tumor necrosis factor-α (TNF-α) were compared between the two groups. Results (1) EA intervention significantly improved high quality embryo rate of PCOS patients (P〈0.05), and clinical pregnancy rate was increased by 8.36%; (2) EA intervention significantly reduced the TNF-α levels of follicular fluid (13.61±15.46 vs 34.09±93.53, P〈0.05); (3) TNF-α levels of serum and follicular fluid in the pregnancy group were lower than those of non-pregnancy group [pregnancy group: (53.91±63.32) pg/mL, (14.93±25.37) pg/mL, nonpregnancy group: (76.82 ± 82.96) pg/mL, (25.04 ± 35.79) pg/mL], and the differences were significant (both P〈0.05). Conclusion EA improves egg quality of PCOS patients and increases the clinical pregnancy rate of IVF-ET, the mechanism may be related to TNF-α levels.展开更多
Effects of ecdysteroid and juvenile hormone (JH) on vitellogenesis of the Japanese oak silkworm, Antheraea yamamai are reported in this article. After topical treatment with 20 hydroxyecdysone alone or JH analog ...Effects of ecdysteroid and juvenile hormone (JH) on vitellogenesis of the Japanese oak silkworm, Antheraea yamamai are reported in this article. After topical treatment with 20 hydroxyecdysone alone or JH analog (i.e. methoprene) alone and combined treatment with these two chemicals, vitellogenin (Vg) titers in the fat body and haemolymph at the pupal stage were mostly higher than those of the control, indicating that both ecdysteroid and JH exerted a promoting effect on the synthesis of Vg. In contrast, the Vg uptake was markedly inhibited by JH while stimulating effect of the ecdysteroid could be shown that vitellin (Vt) titer in the ovary was lower after methoprene treatments, but higher after 20 hydroxyecdyson treatments. Meanwhile, effects of these two hormones on Vg synthesis in the fat body were also tested with the incubation in vitro with Grace medium containing 3H leucine and the hormones. The results demonstrated that Vg synthesis was stimulated after treating with methoprene alone or 20 hydroxyecdysone alone and combined treating with these two chemicals, and particularly ecdysteroid had more marked positive effect. To comprehensively concluded our results, it could be regarded that ecdysteroid play the main role in the regulation of vitellogenesis for the Japanese oak silkworm.展开更多
Microcarriers have a demonstrated value for biomedical applications,in particular for drug delivery and three-dimensional cell culture.Attempts to develop this technique tend to focus on the fabrication of functional ...Microcarriers have a demonstrated value for biomedical applications,in particular for drug delivery and three-dimensional cell culture.Attempts to develop this technique tend to focus on the fabrication of functional microparticles by using convenient methods with innovative but accessible materials.Inspired by the process of boiling eggs in everyday life,which causes the solidification of egg proteins,we present a new microfluidic‘‘cooking"approach for the generation of egg-derived microcarriers for cell culture and drug delivery.As the egg emulsion droplets are formed with exquisite precision during the microfluidic emulsification,the resultant egg microcarriers present highly monodisperse and uniform morphologies at the size range of hundred microns to one millimeter.Benefiting from the excellent biocompatibility of the egg protein components,the obtained microcarriers showed good performances of cell adherence and growth.In addition,after a freezing treatment,the egg microcarriers were shown to have interconnected porous structures throughout their whole sphere,could absorb and load different kinds of drugs or other active molecules,and work as microcarrier-based delivery systems.These features point to the potential value of the microfluidic egg microcarriers in biomedicine.展开更多
基金supported by Yunnan Key Laboratory of Animal Reproductive Biology(2010-03)
文摘The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome(PCOS),which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS female monkeys.Six adult female rhesus monkeys aged 6-10 a,were divided into a PCOS group and a control group.The PCOS group were given two cycles of subcutaneous injections of propionic acid testosterone(PAT),3.5 mg/kg body weight,on day 1,day 3,and day 5 of the menstrual cycle,respectively,and then given muscle injections of human chorionic gonadotropin(HCG),350 IU/kg body wtight,on day 7,day 9,and day 11,respectively.Results showed that high levels of serum LH and T [(5.35±0.17) IU/L and(7.58±0.14) ng/mL,respectively],and a high ratio value of LH/FSH(5.35/1.30=4.12) were observed in the PCOS group.No significant differences were found in serum FSH,E2,and P in the PCOS group compared with those of the control.Polycystic ovaries in the PCOS monkeys were recorded by live ultrasound.The blastocysts rates of the PCOS vs.the control were 23.53% vs.66.67%,and there was a significant difference between the two groups.This study shows that PAT coupled with HCG can induce PCOS in rhesus monkeys in the short term.The reproductive features of PCOS monkeys were similar to those of PCOS patients.
基金Supported by the Chinese National Natural Scientific Fund(30472226)
文摘Objective:To address how genistein sensitizes the chemotherapy-resistant ovarian carcinoma cells and promotes apoptosis in the respect of cell cycle and the regulation of survivin expression in the process.Methods:Ovarian SKOV-3 carcinoma cell line was treated with genistein or cisplatin either alone or in combination.Cell viability was showed by MTT method.Cell cycle and apoptosis were detected by flow cytometry.Survivin mRNA and protein were revealed by RT-PCR and immunocytochemistry,respectively.Results:Genistein could reduce the cell viability in a dose-dependent manner,while cisplatin did so at a much higher level.In contrast,if the two agents were treated in combination,half growth inhibition(IC50) value for cisplatin was reduced remarkably and the effect was synergistic as analyzed by isobologram.In particular,the reduced cell viability was exhibited by a switch in cell cycle progression,as the cells were arrested in G2/M phase and the G0/G1 phase-fraction was significantly decreased.The reduced cell viability appeared to involve apoptosis,based on our results from flow cytometry and Hoechst 33258 staining.In the meanwhile,genistein performed the inhibitory effect on cisplatin-induced survivin expression.Conclusion:Genistein can sensitize ovarian carcinoma cells to cisplatin therapy with the inhibition of survivin expression as the potential mechanism.
文摘Efficacy of the egg parasitoid, Trichogramma evanescens and the bio-pesticide bacteria, Bacillus thuringiensis kurstaki (Btk) in reduction of lesser date moth, Batrachedra amydraula infestations were studied during seasons of 2011 and 2012. The result of 2011 showed that the release of egg parasitoid by rate of 500 and 1,000 parasitoids/palm tree achieved 55.06% and 67.45% reduction in infestation percentage, respectively. Meanwhile high reduction in infestation was achieved by Btk (78.65%) at dose rate of 6 g Btk powder/kg talc powder. The result of 2012 showed that the highest control efficacy (58.78%) was achieved at the first time of the control process, using Btk at dose rate of 6 g/kg talc powder. This result was not different significantly from the release of egg parasitoid by rate of 1,000 parasitoids/palm tree which achieved 53% reduction in infestation percentage. At the second time (after one week from the first application), the efficacy of Btk at dose rate of 6g/kg talc powder was increased to 78.78%, which differ significantly with the release of egg parasitoid by the same rate of 1,000 parasitoids/palm tree, having 61.79% efficacy. Reapplication of the control method achieved an increase in efficacy of the control agents, such increase was significant for both the release of the parasitoid at rate of 1,000/palm tree and for Btk at a dose of 6 g/kg talc powder.
文摘In present study, a neogregarine from Crioceris asparagi is reported for the first time in Turkey. C. asparagi (common asparagus beetle) is an important pest of Asparagus officinalis L. crops in Turkey and the world. Therefore, determining of the protist pathogens of C. asparagi is aimed. During the study, a total of 1,099 C. asparagi adults were examined thoroughly using a light microscope. Insects were collected from the provinces of Manisa, Eskisehir and Bahkesir in Turkey, with 117 of them collected from Manisa, 204 of them from Bahkesir and 778 of them from Eskisehir. A neogregarine pathogen was found in Bahkesir and Manisa. Total infection rate was 18% for Bahkesir between the years 2014-2015 and 15.3% for Manisa in 2011. Oocysts of the neogregarine pathogen were observed only in malpighian tubes of C. asparagi adults. Fresh mature oocysts of the neogregarine was measured as 9.34 ± 0.74 lam (n = 50) in length and 5.27 ± 0.36 μm (n = 50) in width. Identification of entomopathogens is prerequisite for their use in biological control.
基金Supported by the National Natural Science Foundation of China:30973790
文摘Objective To observe the effect of electroacupuncture (EA) on egg quality of polycystic ovarian syndrome (PCOS) patients, and to explore its mechanism. Methods Two hundred patients who received in vitro fertilization-embryo transfer (IVF-ET) were divided into an EA group (102 cases) and a control group (98 cases) according to random number table. All the patients in the two groups were given Diane-35 and gonadotropin-releasing hormone agonist (GnRH-α) for ovarian hyperstimulation. Besides, EA intervention was applied to Shenshu (肾俞 BL 23), Qihai (气海 GV 6), Zusanli (足三里ST 36), Sanyinjiao (三阴交 SP 6), Neiguan (内关 PC 6) and Zigong (子宫 EX-CA1), etc. in the EA group. Then egg quality, final outcome of pregnancy and levels of tumor necrosis factor-α (TNF-α) were compared between the two groups. Results (1) EA intervention significantly improved high quality embryo rate of PCOS patients (P〈0.05), and clinical pregnancy rate was increased by 8.36%; (2) EA intervention significantly reduced the TNF-α levels of follicular fluid (13.61±15.46 vs 34.09±93.53, P〈0.05); (3) TNF-α levels of serum and follicular fluid in the pregnancy group were lower than those of non-pregnancy group [pregnancy group: (53.91±63.32) pg/mL, (14.93±25.37) pg/mL, nonpregnancy group: (76.82 ± 82.96) pg/mL, (25.04 ± 35.79) pg/mL], and the differences were significant (both P〈0.05). Conclusion EA improves egg quality of PCOS patients and increases the clinical pregnancy rate of IVF-ET, the mechanism may be related to TNF-α levels.
文摘Effects of ecdysteroid and juvenile hormone (JH) on vitellogenesis of the Japanese oak silkworm, Antheraea yamamai are reported in this article. After topical treatment with 20 hydroxyecdysone alone or JH analog (i.e. methoprene) alone and combined treatment with these two chemicals, vitellogenin (Vg) titers in the fat body and haemolymph at the pupal stage were mostly higher than those of the control, indicating that both ecdysteroid and JH exerted a promoting effect on the synthesis of Vg. In contrast, the Vg uptake was markedly inhibited by JH while stimulating effect of the ecdysteroid could be shown that vitellin (Vt) titer in the ovary was lower after methoprene treatments, but higher after 20 hydroxyecdyson treatments. Meanwhile, effects of these two hormones on Vg synthesis in the fat body were also tested with the incubation in vitro with Grace medium containing 3H leucine and the hormones. The results demonstrated that Vg synthesis was stimulated after treating with methoprene alone or 20 hydroxyecdysone alone and combined treating with these two chemicals, and particularly ecdysteroid had more marked positive effect. To comprehensively concluded our results, it could be regarded that ecdysteroid play the main role in the regulation of vitellogenesis for the Japanese oak silkworm.
基金supported by the National Natural Science Foundation of China (21473029, 51522302)the NSAF Foundation of China (U1530260)+2 种基金the Natural Science Foundation of Jiangsu Province (BK20140028)the Program for New Century Excellent Talents in Universitythe Scientific Research Foundation of Southeast University
文摘Microcarriers have a demonstrated value for biomedical applications,in particular for drug delivery and three-dimensional cell culture.Attempts to develop this technique tend to focus on the fabrication of functional microparticles by using convenient methods with innovative but accessible materials.Inspired by the process of boiling eggs in everyday life,which causes the solidification of egg proteins,we present a new microfluidic‘‘cooking"approach for the generation of egg-derived microcarriers for cell culture and drug delivery.As the egg emulsion droplets are formed with exquisite precision during the microfluidic emulsification,the resultant egg microcarriers present highly monodisperse and uniform morphologies at the size range of hundred microns to one millimeter.Benefiting from the excellent biocompatibility of the egg protein components,the obtained microcarriers showed good performances of cell adherence and growth.In addition,after a freezing treatment,the egg microcarriers were shown to have interconnected porous structures throughout their whole sphere,could absorb and load different kinds of drugs or other active molecules,and work as microcarrier-based delivery systems.These features point to the potential value of the microfluidic egg microcarriers in biomedicine.