[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid rati...[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.展开更多
To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at ...To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at (100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw) against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters (e.g., hemoglobin content, red and white blood cells count and differential cell count) after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose (P 〈 0.05). It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.展开更多
基金Supported by the Key Scientific and Technological Project of Henan Province (102102110157)the Scientific Research Found Project of Henan University of Urban Construction (2010JZD008)~~
文摘[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.
文摘To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at (100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw) against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters (e.g., hemoglobin content, red and white blood cells count and differential cell count) after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose (P 〈 0.05). It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.