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紫菜多糖提取工艺优化研究 被引量:4
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作者 邓红霞 邬应龙 +1 位作者 刘青梅 杨性民 《食品工业科技》 CAS CSCD 北大核心 2005年第1期129-130,共2页
应用微波技术,采取L9(34)正交优化实验,研究了微波功 率、提取加热时间和液固重量比对紫菜粗多糖提取率的 影响。结果表明,微波提取紫菜多糖最优工艺条件为:微 波功率200w、提取时间8min、水与紫菜的液固重量比为 50:1。
关键词 紫菜多糖 微波提取 微波功率 固重量比 提取时间
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高效液相色谱串联原子荧光法测定鱼类甲基汞的两种前处理方法比较与分析 被引量:1
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作者 李继源 孔佳 +2 位作者 柯异芸 肖新峰 包斌 《上海农业学报》 CSCD 2016年第4期81-86,共6页
分别采用超声波辅助提取和提取液提取方法对鱼体中的甲基汞进行前处理,通过高效液相色谱串联原子荧光法分析测定甲基汞含量。结果表明:两种方法均可用于高效液相色谱串联原子荧光法测定鱼类甲基汞的前处理。超声辅助提取和提取液提取的... 分别采用超声波辅助提取和提取液提取方法对鱼体中的甲基汞进行前处理,通过高效液相色谱串联原子荧光法分析测定甲基汞含量。结果表明:两种方法均可用于高效液相色谱串联原子荧光法测定鱼类甲基汞的前处理。超声辅助提取和提取液提取的回收率分别为81%—95%和82%一1叭%;精密度分别为6.7%和5.6%;检测限分别为33.5μg/kg和13.4μg/kg;两种前处珲方法测定FAPAS样品得列的测定结果与定值吻合,准确度满足分析要求。超声波辅助提取相对提取液提取简便、经济性较好,而提取液提取相对超声波辅助提取试验耗时短、检出限低,在对未知浓度甲基汞(痕量)进行测定时,提取液提取相对超声波辅助提取更为可靠。 展开更多
关键词 甲基汞 鱼肉 高效相色谱串联原子荧光法 超声波辅助提取 提取液提取
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Extraction of Pumpkin Polysaccharide by Complex Enzyme Method and Its Antioxidant Research 被引量:20
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作者 孙婕 尹国友 +2 位作者 陈兰英 Guo-you Lan-ying 《Agricultural Science & Technology》 CAS 2010年第5期34-37,共4页
[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid rati... [Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide. 展开更多
关键词 Complex enzymes method Pumpkin polysaccharide Extraction Antioxidant activity
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Inhibition of Ehrlich Ascites Carcinoma by Lactuca serriola in Swiss Albino Mice
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作者 Mona Alshathly Eman Elsharkawy 《Journal of Chemistry and Chemical Engineering》 2014年第1期66-71,共6页
To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at ... To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at (100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw) against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters (e.g., hemoglobin content, red and white blood cells count and differential cell count) after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose (P 〈 0.05). It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity. 展开更多
关键词 Antitumor activity Lactuca serriola EAC hematological parameter.
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