Currently in China,no technically and economically viable methods exist to handle large quantities of Camptotheca acuminata Decne residue(CA residue) after camptothecin extract while there is a great demand for low co...Currently in China,no technically and economically viable methods exist to handle large quantities of Camptotheca acuminata Decne residue(CA residue) after camptothecin extract while there is a great demand for low cost alternatives to replace the cottonseed hull-based materials used in commercial mushroom culture.Hence,it is of importance for camptothecin extract factories and mushroom producers to explore the utilization of CA residue in mushroom industry.We conducted a research to study how partially or completely substituting traditional mushroom substrate by CA residue would influence the mycelial growth in mushroom spawn production.5 mushroom strains from 4 species were used in the test,i.e.,oyster mushroom(Pleurotus ostreatu) strains,Zayou No.1 and Xide 33,needle mushroom(Flammulina velutipes) strain Chuanjin No.3,hairy wood ear(Auricularia polytricha) strain Huang Er No.10,and shiitake(Lentinula edodes) strain Wuxiang.The nutrient element composition and heavy metal contents of CA residue were determined to ensure its safety and to determine its appropriate component in the substrate formulation for mushroom spawn production.The four substrate formulations(one control-CK,and three treatments,named,T1,T2,T3,) contained 0%,40%,79%,and 100% CA residue,respectively,to allow comparison of the fungal mycelial growth.The control(CK) was the popularly used formulation in Chinese commercial mushroom production,comprising of 73% cottonseed hulls,10% sawdust,15% wheat bran,1% lime,1% white sugar(percentage by weight).All mushroom spawns of the five strains in the four treatments were incubated under the same conditions.The results showed that mycelia of the five mushroom strains grew significantly faster on the substrates containing CA residue than on the substrate with no CA residue(CK).There were no significant differences in the mycelial growth rate among treatments containing CA residue for the two oyster mushrooms and the needle mushroom,but mycelial growth rate in treatments T2 and T3 was significantly higher than in treatment T1 for hairy wood ear and shiitake.The results suggest that CA residue can be used to culture oyster mushroom,needle mushroom,hairy wood ear,and shiitake spawn,and the medium containing CA residue can stimulate their mycelial growth.The commercial production of mushroom spawn using CA residue not only brings better economical benefits including lower cost to mushroom producers,but also reduces environmental pollution by providing a means to reduce dumping and piling of CA residue.展开更多
Biodiesel, which is a renewable and environmentally friendly fuel, has been studied widely to help remedy increasing environmental problems. One of the key processes of biodiesel production is oil extraction from oils...Biodiesel, which is a renewable and environmentally friendly fuel, has been studied widely to help remedy increasing environmental problems. One of the key processes of biodiesel production is oil extraction from oilseed materials. Switchable solvents can reversibly change from molecular to ionic solvents under atmospheric CO_2,and can be used for oil extraction. N, N-dimethylcyclohexylamine(DMCHA), a switchable solvent, was used to extract oil from Jatropha curcas L. oil seeds to produce biodiesel. The appropriate extraction conditions were:1:2 ratio of seed mass to DMCHA volume, 0.3–1 mm particle size, 200 r·min-1agitation speed, 60 min extraction time, and 30 °C extraction temperature. The extraction ratio was about 83%. This solvent extracted the oil more efficiently than hexane, and is much less volatile. By bubbling CO_2 under 1 atm and 25 °C for 5 h, the oil was separated, and DMCHA was recovered after releasing CO_2 by bubbling N_2 under 1 atm and 60 °C for 2 h. The residual solvent content in oil was about 1.7%. Selectivity of DMCHA was evaluated by detecting the protein and sugar content in oil. Using the oil with residual solvent to conduct transesterification process, the oil conversion ratio was approximately 99.5%.展开更多
This research focused using novel substrate, longan Lactobacillus casei subsp, rhamnosus TISTR 108. The optimum juice as carbon source for continuous lactic acid production by medium for lactic acid production was pur...This research focused using novel substrate, longan Lactobacillus casei subsp, rhamnosus TISTR 108. The optimum juice as carbon source for continuous lactic acid production by medium for lactic acid production was pure longan juice with 120 g/L sugar concentration and among the different nitrogen sources were added to the longan juice （yeast extract, tryptic soy, urea, （NH4）2SO4 and NaNO3）, yeast extract had the most efficiency. Yeast extract （10 g/L） without adding minerals to longan juice could produced the maximum lactic acid concentration of 38.91 ± 0.190 g/L in 60 h and the yield of 0.460± 0.122 g/g with the productivity of 0.649± 0.002 g/Lh in 2 liters flask. Batch fermentation was conducted in 2 liters fermentor and 41.38± 0.030 g/L lactic acid was produced in 48 h with the yield of 0.398 ± 0.215 g/g and the productivity was 0.862 ± 0.001 g/L h. The continuous fermentation using 2 liters fermentor as a high productivity for lactic acid （1.091 ± 0.001 g/L h） was achieved at dilution rate （D） of 0.0685 h-1.展开更多
Mango fruit （Mangifera indica） is very rich in fibre and vitamin C which are the required nutrients for good health and easy digestion of food in human body system. However, the fruit is highly perishable in its nat...Mango fruit （Mangifera indica） is very rich in fibre and vitamin C which are the required nutrients for good health and easy digestion of food in human body system. However, the fruit is highly perishable in its natural state after harvest due to the chemical deterioration and environmental effects. Due to the perishable nature, abundant wastage during the production season and relative scarcity during the off season were recorded by farmers. Processing the fruit into the form that can easily be stored, preserved, packaged, transported or consumed is crucial to having the product all the year round. Besides, mango juice can be consumed freshly, processed into dry powder, mixed or blended with other juice to make fruit jams, or evaporated to concentrates. These products have a lot of potential in food and beverage industries for export and foreign exchange earnings. Therefore, a small scale machine to process mango fruits to high quality juice is highly essential to reduce the postharvest loss and then add value to the commodity. To achieve this purpose, an abrasion-macerating device （AMD） was designed, fabricated and tested for small scale mango juice extraction. Design considerations focused on the techno-economic status of the micro and small scale fruit juice processors who are the intended users of the machine. The major components of the machine included hopper, perforated drum, screw conveyor, juice outlet, waste outlet, frame, electric motor and motor stand. Other components included screw shaft, the juice collector, top cover and the transmission system. In operation, the screw conveyor conveys and presses the mango fruits against the perforated roughened drum. The abrasion/tearing process of the screw on the flesh of the fruit and further pressing against the drum squeeze enough juice out of the fruit. The juice extracted is drained through the perforated mesh of the juice channel into the juice outlet from where it is collected while the residual waste is collected at the waste outlet. The machine was tested using freshly harvested mango fruits and results obtained showed an average juice yield, extraction efficiency and extraction loss of 34,56%, 55.14% and 10.15%, respectively. These values of juice yield, extraction efficiency and low level of extraction loss indicate satisfactory performance of the machine. Powered by a 2.5 hp single-phase electric motor, the machine has a production cost of USD565 with the construction materials being locally available at affordable costs. A cottage mango juice extraction plant based on this technology can provide employment for at least two persons at the same time providing fresh juice at low costs and residual waste as an ingredient for livestock feed manufacturing.展开更多
基金funded by theCrop Breeding Key Project of Sichuan Province(Grant No. 2006YZGG 26)
文摘Currently in China,no technically and economically viable methods exist to handle large quantities of Camptotheca acuminata Decne residue(CA residue) after camptothecin extract while there is a great demand for low cost alternatives to replace the cottonseed hull-based materials used in commercial mushroom culture.Hence,it is of importance for camptothecin extract factories and mushroom producers to explore the utilization of CA residue in mushroom industry.We conducted a research to study how partially or completely substituting traditional mushroom substrate by CA residue would influence the mycelial growth in mushroom spawn production.5 mushroom strains from 4 species were used in the test,i.e.,oyster mushroom(Pleurotus ostreatu) strains,Zayou No.1 and Xide 33,needle mushroom(Flammulina velutipes) strain Chuanjin No.3,hairy wood ear(Auricularia polytricha) strain Huang Er No.10,and shiitake(Lentinula edodes) strain Wuxiang.The nutrient element composition and heavy metal contents of CA residue were determined to ensure its safety and to determine its appropriate component in the substrate formulation for mushroom spawn production.The four substrate formulations(one control-CK,and three treatments,named,T1,T2,T3,) contained 0%,40%,79%,and 100% CA residue,respectively,to allow comparison of the fungal mycelial growth.The control(CK) was the popularly used formulation in Chinese commercial mushroom production,comprising of 73% cottonseed hulls,10% sawdust,15% wheat bran,1% lime,1% white sugar(percentage by weight).All mushroom spawns of the five strains in the four treatments were incubated under the same conditions.The results showed that mycelia of the five mushroom strains grew significantly faster on the substrates containing CA residue than on the substrate with no CA residue(CK).There were no significant differences in the mycelial growth rate among treatments containing CA residue for the two oyster mushrooms and the needle mushroom,but mycelial growth rate in treatments T2 and T3 was significantly higher than in treatment T1 for hairy wood ear and shiitake.The results suggest that CA residue can be used to culture oyster mushroom,needle mushroom,hairy wood ear,and shiitake spawn,and the medium containing CA residue can stimulate their mycelial growth.The commercial production of mushroom spawn using CA residue not only brings better economical benefits including lower cost to mushroom producers,but also reduces environmental pollution by providing a means to reduce dumping and piling of CA residue.
基金Supported by Doctoral Fund of Ministry of Education of China(20130181130006)the National Natural Science Foundation of China(No.21476150)
文摘Biodiesel, which is a renewable and environmentally friendly fuel, has been studied widely to help remedy increasing environmental problems. One of the key processes of biodiesel production is oil extraction from oilseed materials. Switchable solvents can reversibly change from molecular to ionic solvents under atmospheric CO_2,and can be used for oil extraction. N, N-dimethylcyclohexylamine(DMCHA), a switchable solvent, was used to extract oil from Jatropha curcas L. oil seeds to produce biodiesel. The appropriate extraction conditions were:1:2 ratio of seed mass to DMCHA volume, 0.3–1 mm particle size, 200 r·min-1agitation speed, 60 min extraction time, and 30 °C extraction temperature. The extraction ratio was about 83%. This solvent extracted the oil more efficiently than hexane, and is much less volatile. By bubbling CO_2 under 1 atm and 25 °C for 5 h, the oil was separated, and DMCHA was recovered after releasing CO_2 by bubbling N_2 under 1 atm and 60 °C for 2 h. The residual solvent content in oil was about 1.7%. Selectivity of DMCHA was evaluated by detecting the protein and sugar content in oil. Using the oil with residual solvent to conduct transesterification process, the oil conversion ratio was approximately 99.5%.
文摘This research focused using novel substrate, longan Lactobacillus casei subsp, rhamnosus TISTR 108. The optimum juice as carbon source for continuous lactic acid production by medium for lactic acid production was pure longan juice with 120 g/L sugar concentration and among the different nitrogen sources were added to the longan juice （yeast extract, tryptic soy, urea, （NH4）2SO4 and NaNO3）, yeast extract had the most efficiency. Yeast extract （10 g/L） without adding minerals to longan juice could produced the maximum lactic acid concentration of 38.91 ± 0.190 g/L in 60 h and the yield of 0.460± 0.122 g/g with the productivity of 0.649± 0.002 g/Lh in 2 liters flask. Batch fermentation was conducted in 2 liters fermentor and 41.38± 0.030 g/L lactic acid was produced in 48 h with the yield of 0.398 ± 0.215 g/g and the productivity was 0.862 ± 0.001 g/L h. The continuous fermentation using 2 liters fermentor as a high productivity for lactic acid （1.091 ± 0.001 g/L h） was achieved at dilution rate （D） of 0.0685 h-1.
文摘Mango fruit （Mangifera indica） is very rich in fibre and vitamin C which are the required nutrients for good health and easy digestion of food in human body system. However, the fruit is highly perishable in its natural state after harvest due to the chemical deterioration and environmental effects. Due to the perishable nature, abundant wastage during the production season and relative scarcity during the off season were recorded by farmers. Processing the fruit into the form that can easily be stored, preserved, packaged, transported or consumed is crucial to having the product all the year round. Besides, mango juice can be consumed freshly, processed into dry powder, mixed or blended with other juice to make fruit jams, or evaporated to concentrates. These products have a lot of potential in food and beverage industries for export and foreign exchange earnings. Therefore, a small scale machine to process mango fruits to high quality juice is highly essential to reduce the postharvest loss and then add value to the commodity. To achieve this purpose, an abrasion-macerating device （AMD） was designed, fabricated and tested for small scale mango juice extraction. Design considerations focused on the techno-economic status of the micro and small scale fruit juice processors who are the intended users of the machine. The major components of the machine included hopper, perforated drum, screw conveyor, juice outlet, waste outlet, frame, electric motor and motor stand. Other components included screw shaft, the juice collector, top cover and the transmission system. In operation, the screw conveyor conveys and presses the mango fruits against the perforated roughened drum. The abrasion/tearing process of the screw on the flesh of the fruit and further pressing against the drum squeeze enough juice out of the fruit. The juice extracted is drained through the perforated mesh of the juice channel into the juice outlet from where it is collected while the residual waste is collected at the waste outlet. The machine was tested using freshly harvested mango fruits and results obtained showed an average juice yield, extraction efficiency and extraction loss of 34,56%, 55.14% and 10.15%, respectively. These values of juice yield, extraction efficiency and low level of extraction loss indicate satisfactory performance of the machine. Powered by a 2.5 hp single-phase electric motor, the machine has a production cost of USD565 with the construction materials being locally available at affordable costs. A cottage mango juice extraction plant based on this technology can provide employment for at least two persons at the same time providing fresh juice at low costs and residual waste as an ingredient for livestock feed manufacturing.
