The research of the category and extraction methods of effective ingredients of fish scale was reviewed at home and abroad. The extraction process of fish scale was studied, including collagen, hydroxyapatite, lecithi...The research of the category and extraction methods of effective ingredients of fish scale was reviewed at home and abroad. The extraction process of fish scale was studied, including collagen, hydroxyapatite, lecithin, guanine etc. The different extraction methods and the components were analyzed. The research of effective ingredients of fish scales was reviewed as theory basis to further research and comprehensive development.展开更多
In current palaeodietary research, gelatinization is the main method to extract insoluble collagen （ISC） from ancient bones. However, the degradation products of ISC, i.e., soluble collagen （SC）, is often neglecte...In current palaeodietary research, gelatinization is the main method to extract insoluble collagen （ISC） from ancient bones. However, the degradation products of ISC, i.e., soluble collagen （SC）, is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be at- tributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ13c and δ15N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range （2.9-3.6）, the mean difference of δ13C value was only （0.3±0.2）%o （n=2） while δ15N value was （0.6±0.1）‰ （n=2）. Although the atomic C/N ratios of some SC are slightly be- yond the normal range, the mean differences of δ13C and δ15N values were still only （0.4±0.1）‰and （0.3±0）‰ （n=2） respec- tively. These isotopic differences are quite below the isotope fractionation in one trophic level δ13C values of 1‰-1.5‰ and 615N values of 3‰-5‰）, suggesting that SC had great application potentials in palaeodietary research.展开更多
文摘The research of the category and extraction methods of effective ingredients of fish scale was reviewed at home and abroad. The extraction process of fish scale was studied, including collagen, hydroxyapatite, lecithin, guanine etc. The different extraction methods and the components were analyzed. The research of effective ingredients of fish scales was reviewed as theory basis to further research and comprehensive development.
基金supported by CAS Strategic Priority Research Program (Grant No. XDA05130303)Chinese Academy of Sciences & Max-Planck Institute Partnership Group Project (Grant No. KACX1-YW-0830)
文摘In current palaeodietary research, gelatinization is the main method to extract insoluble collagen （ISC） from ancient bones. However, the degradation products of ISC, i.e., soluble collagen （SC）, is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be at- tributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ13c and δ15N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range （2.9-3.6）, the mean difference of δ13C value was only （0.3±0.2）%o （n=2） while δ15N value was （0.6±0.1）‰ （n=2）. Although the atomic C/N ratios of some SC are slightly be- yond the normal range, the mean differences of δ13C and δ15N values were still only （0.4±0.1）‰and （0.3±0）‰ （n=2） respec- tively. These isotopic differences are quite below the isotope fractionation in one trophic level δ13C values of 1‰-1.5‰ and 615N values of 3‰-5‰）, suggesting that SC had great application potentials in palaeodietary research.
