The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column w...The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.展开更多
In this paper, silt sediment is considered to be Bingham body, which is made up of coarse and fine particles in front of a hydraulic gate. The coarse and fine particles provide friction and shear stress in the course ...In this paper, silt sediment is considered to be Bingham body, which is made up of coarse and fine particles in front of a hydraulic gate. The coarse and fine particles provide friction and shear stress in the course of opening the gate. They constitute together the adhesion force of the sediment. Based on this viewpoint, this paper puts forward a formula for the effect of silt sediment on the lifting force. The formula includes gate weight, down-suction force, sealing rubber friction, plus-weight, water-column pressure, plus-silted-sediment weight and rolling(or sliding)-bearing friction. Finally, the verification results show that the formula has certain reliability and the calculation accuracy can meet the need of practical engineering.展开更多
In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （...In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （XEZKTJ） with standardized reference extract （SRE）. The five analytes （liquiritin apioside, liquiritin, isoliquiritin apioside, liquirigenin and glycyrrhizic acid） were well separated with good linearity, precision, stability and repeatability. The recovery rates ranged from 95.69% to 100.80%. The content of the five compounds in 34 batches of commercial XEZKTJ products was determined using standardized GRR extract （SRE method） and individual chemical reference standards （CRS method）. Highly similar results were obtained from the two methods, demonstrating the feasibility of the proposed SRE method. Taken together, we proposed an efficient and low-cost way to perform multi-component quality control of XEZKTJ in this study.展开更多
基金This paper was supported by National Natural Science Foundation of China (No. 30070086).
文摘The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.
基金Supported by Shandong Province Outstanding Young Scientist Award Fund(No.BS2014SF016)the National Natural Science Foundation of China(No.50979067)+1 种基金Ludong University Doctor Introduction Foundation(No.LY2014026 and No.LY2014028)the Open Research Fund Program of State Key Laboratory of Water Resources and Hydropower Engineering Science(No.2012B104)
文摘In this paper, silt sediment is considered to be Bingham body, which is made up of coarse and fine particles in front of a hydraulic gate. The coarse and fine particles provide friction and shear stress in the course of opening the gate. They constitute together the adhesion force of the sediment. Based on this viewpoint, this paper puts forward a formula for the effect of silt sediment on the lifting force. The formula includes gate weight, down-suction force, sealing rubber friction, plus-weight, water-column pressure, plus-silted-sediment weight and rolling(or sliding)-bearing friction. Finally, the verification results show that the formula has certain reliability and the calculation accuracy can meet the need of practical engineering.
基金Drug Standards Improvement Project of Chinese Pharmacopoeia Commission
文摘In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （XEZKTJ） with standardized reference extract （SRE）. The five analytes （liquiritin apioside, liquiritin, isoliquiritin apioside, liquirigenin and glycyrrhizic acid） were well separated with good linearity, precision, stability and repeatability. The recovery rates ranged from 95.69% to 100.80%. The content of the five compounds in 34 batches of commercial XEZKTJ products was determined using standardized GRR extract （SRE method） and individual chemical reference standards （CRS method）. Highly similar results were obtained from the two methods, demonstrating the feasibility of the proposed SRE method. Taken together, we proposed an efficient and low-cost way to perform multi-component quality control of XEZKTJ in this study.
