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文昌鸡资源调查与提纯繁殖总结报告
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作者 叶保国 林哲敏 《海南农业科技》 1999年第4期10-11,15,共3页
关键词 文昌鸡 资源调查 文昌市 提纯繁殖技术
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水稻光温敏核不育系提纯繁殖技术研究进展 被引量:3
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作者 何强 邓华凤 +3 位作者 庞震宇 舒服 张武汉 杨益善 《杂交水稻》 CSCD 北大核心 2010年第6期1-3,7,共4页
分析了水稻光温敏核不育系需要进行提纯繁殖的原因及提纯繁殖的技术可行性,综述了现有水稻光温敏核不育系提纯繁殖技术特点和存在的问题,探讨了水稻光温敏核不育系的提纯和原种生产程序实现工厂化、商业化和专业化生产的技术可能性。
关键词 水稻光温敏核不育系 提纯繁殖 起点温度 漂变
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水稻光温敏不育系“六圃法”提纯保真原种繁殖体系的建立与应用 被引量:5
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作者 吴俊 邓启云 +3 位作者 齐绍武 庄文 石祖兴 周川广 《中国水稻科学》 CAS CSCD 北大核心 2017年第1期99-104,共6页
【目的】水稻光温敏不育系的提纯保真繁殖对于两系法杂交水稻的发展和应用具有十分重要的意义。现有提纯繁殖技术多以防止起点温度漂移为目的,而忽视了生物学性状的提纯保真。为解决这些问题,本研究结合光温敏不育系Y58S的多年大规模原... 【目的】水稻光温敏不育系的提纯保真繁殖对于两系法杂交水稻的发展和应用具有十分重要的意义。现有提纯繁殖技术多以防止起点温度漂移为目的,而忽视了生物学性状的提纯保真。为解决这些问题,本研究结合光温敏不育系Y58S的多年大规模原种生产实践,建立了水稻光温敏不育系"六圃法"提纯保真原种繁殖体系。【方法】该技术体系基本步骤如下:首先,直接从生产上大规模制种使用的不育系种子中取样,种植"典型单株筛选圃",进行典型单株初选;其次,通过"起点温度鉴定圃"筛选不育起点温度基本一致的核心单株,进行起点温度纯化;然后,通过"群体观察圃"、"配合力鉴定圃"和"制种试验圃"来确保光温敏不育系的遗传背景基本纯合和表型整齐一致,从而决选保真核心株系,进而获得保真核心种子;最后,建立连续稳定的品种"专一性原种繁殖圃"来实现原原种和原种的高纯度生产。【结论】实践证明,该技术体系可确保获得育性稳定、表型整齐,且遗传背景来源基本一致的光温敏不育系原种,大幅度提高水稻光温敏不育系繁殖的安全可靠性和生产效率。2008年以来,应用该技术体系生产了249万kg Y58S生产用种,基本消灭了同形可育株,纯度全部达到99.5%以上。目前这些Y58S生产用种已经全部销售给各种业公司进行杂交制种,生产的杂交种子推广1474万hm2以上。 展开更多
关键词 水稻 光温敏不育系 提纯保真繁殖体系 Y58S
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Research on a Method for Purification and Multiplication of PTGMS Lines
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作者 何强 庞镇宇 +4 位作者 邢俊杰 舒服 张武汉 杨益善 邓华凤 《Agricultural Science & Technology》 CAS 2014年第3期352-354,366,共4页
[Objective] The aims was to explore PTGMS line purification and multipli- cation technology. [Method] Three commercial PTGMS lines, Annong 810S, P88S and C815S, were used in this study. In the first year, two sterile ... [Objective] The aims was to explore PTGMS line purification and multipli- cation technology. [Method] Three commercial PTGMS lines, Annong 810S, P88S and C815S, were used in this study. In the first year, two sterile line populations with same genetic background were multiplied by dividing seedling; meanwhile, se- lection and multiplication of the core individuals were conducted; within the year, the core seeds harvested were multiplied under the isolated condition in Hainan, and at the same time, superior individuals were selected and used as the seeds for two populations with same genetic background in the next year. In the second year, seeds harvested in Hainan were planted in Changsha, while the core individuals were selected and multiplied; the core seeds harvested were continued to multiply in Hainan. The above procedures were repeated in the following years. [Result] The test results showed that in two years the proportion of core individuals in the popu- lation of Annong 810S, P88S and C815S increased from 40.3%, 0.7% and 0.3% in the first year to 71.67%, 66.00% and 68.67% in the second year respectively. [Conclusion] This suggested that this method of purification and multiplication of the PTGMS lines for consecutive years can effectively control the drift of the critical sterility inducing temperature, and at the same time, core seeds with high purity and controlled inducing temperature can be harvested. 展开更多
关键词 PTGMS PURIFICATION MULTIPLICATION RICE
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