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苜蓿多糖的提纯组分对HIV逆转录酶和蛋白酶活性的抑制作用 被引量:7
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作者 张丽萍 张东杰 冯昆 《中国食品学报》 EI CAS CSCD 2006年第2期59-62,共4页
目的:研究苜蓿初步提纯组分的得率及纯化组分对HIV逆转录酶和蛋白酶活性的体外抑制效果。方法:使用热水浸提法提取芷蓿粗多糖,经DEAE-纤维素柱分段洗脱,得S1、S2、S3、S44种组分;采用试剂盒对体外HIV逆转录酶和蛋白酶活性的抑制作用进... 目的:研究苜蓿初步提纯组分的得率及纯化组分对HIV逆转录酶和蛋白酶活性的体外抑制效果。方法:使用热水浸提法提取芷蓿粗多糖,经DEAE-纤维素柱分段洗脱,得S1、S2、S3、S44种组分;采用试剂盒对体外HIV逆转录酶和蛋白酶活性的抑制作用进行检测比较。结果:S3和S4对HIV逆转录酶活性的抑制率较高,分别为99.6%和76.7%;S1、S2对HIV蛋白酶活性的抑制率较高,分别为58.6%和55.9%。结论:S3和S4表现出对HIV逆转录酶活性有很强的抑制作用,S1、S2对HIV蛋白酶活性有较强的抑制作用。 展开更多
关键词 苜蓿多糖 提纯组分 HIV逆转录酶 HIV蛋白酶 抑制率
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Operation of a Batch Stripping Distillation Column 被引量:7
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作者 许松林 JoseEspinosa +1 位作者 HectorE.Salomone OscarA.Iribarren 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2001年第2期141-144,共4页
A stripping batch distillation column is preferred when the amount of the light component in the feed is small and the products are to be recovered at high purity. The operation modes of a batch stripping are believed... A stripping batch distillation column is preferred when the amount of the light component in the feed is small and the products are to be recovered at high purity. The operation modes of a batch stripping are believed to be the same as those of a rectifier. However, the control system of a stripper is different. In this paper, we explore three different control methods with Hysys (Hyprotech Ltd. 1997) for a batch stripper. The main difference is the control scheme for reboiler liquid level: (a) controlled by reflux flow; (b) controlled by reboiler heat duty; (c) controlled by bottom product flow. The main characteristics of operating a batch stripper with different control scheme are presented in this paper. Guidelines are provided for the startup of a batch stripper, the effects of somecontrol tuning parameters on the column performance are discussed. 展开更多
关键词 batch stripper CONTROL operation
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Antiviral activities against influenza virus(FM1) of bioactive fractions and representative compounds extracted from Banlangen(Radix Isatidis) 被引量:21
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作者 Xiao Ping Ye Weiyang +1 位作者 Chen Jianwei Li Xiang 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2016年第3期369-376,共8页
OBJECTIVE: To study the antiviral activities of clemastanin B(CB), epigoitrin, phenylpropanoids portion(PEP) and the mixture of phenylpropanoids, alkaloids and organic acid fractions(PEP+ALK+OA)from Banlangen(Radix Is... OBJECTIVE: To study the antiviral activities of clemastanin B(CB), epigoitrin, phenylpropanoids portion(PEP) and the mixture of phenylpropanoids, alkaloids and organic acid fractions(PEP+ALK+OA)from Banlangen(Radix Isatidis).METHODS: The experiment consisted of four parts:therapeutic action, prophylaxsis action, inhibition of virus attachment, and direct virucidal action. Cytopathic effect(CPE) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) were used to assess antiviral activity.RESULTS: CB, epigoitrin, PEP and PEP + ALK + OA fractions from Banlangen(Radix Isatidis) extract significantly increased the viability of MDCK cells pre-infected with the virus compared with the virus control group in all the dilutions(P < 0.01). Pretreated with either pure compounds or chemical frac-tions of Banlangen(Radix Isatidis) extract in all the dilutions significantly improved the viability of MDCK cells(P < 0.01). The inhibition of virus absorption to the host cells by CB, epigoitrin and PEP was in a dose dependent manner.CONCLUSION: CB, epigoitrin, PEP and PEP+ALK+OA exert their anti-influenza activity by inhibiting the virus multiplication, prophylaxsis and blocking the virus attachment. The primary mode of action of PEP and PEP + ALK + OA is the inhibition of virus replication. The inhibitory effects on virus attachment and multiplication are the main modes for epigoitrin. 展开更多
关键词 Radix Isatidis Clemastanin B Goitrin Antiviral agents Orthomyxoviridae
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