The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exp...The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR .This implies that the on line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large scale cultivation of mammalian cells.展开更多
摄氧率(OUR)不仅易于在线检测而且可以快速反映细胞的物质和能量代谢状况。为此,利用2 L B.Braun反应器的恒速流加培养工艺,考察了杂交瘤细胞的摄氧率与生长、代谢之间的关系。OUR与活细胞密度成良好的正相关性,但由于氧比消耗速率不是...摄氧率(OUR)不仅易于在线检测而且可以快速反映细胞的物质和能量代谢状况。为此,利用2 L B.Braun反应器的恒速流加培养工艺,考察了杂交瘤细胞的摄氧率与生长、代谢之间的关系。OUR与活细胞密度成良好的正相关性,但由于氧比消耗速率不是常数,所以二者不呈线性关系,从而利用OUR只能估测,而不能准确计算细胞密度。随着葡萄糖浓度降至低水平,氧比消耗速率、ATP比生成速率下降,而氧与葡萄糖的消耗计量比、细胞对氧的消耗逐增,由氧化磷酸化生成的ATP比例上升,说明葡萄糖代谢途径逐渐从糖酵解途径迁移到三羧酸循环,被氧化程度提高,能量利用率增强。展开更多
Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based ...Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based on pseudo-steady state mass transfer balance, an oxygen transfer model was established.Volumetric oxygen transfer coefficient k_La was estimated as a function of aeration rate and power input per unit volume.Oxygen uptake rate obtained from the macrokinetic model matched well with the oxygen transfer rate estimated by the mass balance model during the whole cultivation.The application of the oxygen transfer model for on-line biomass estimation was also presented.展开更多
目的探讨醒脑静注射液在体外循环心脏手术中对患者脑功能的影响。方法选择心脏瓣膜手术患者30例,随机分为实验组和对照组,各15例。实验组在体外循环预充液中按0.33 m L/kg加入醒脑静注射液,对照组加入等量0.9%氯化钠溶液,分别于体外循...目的探讨醒脑静注射液在体外循环心脏手术中对患者脑功能的影响。方法选择心脏瓣膜手术患者30例,随机分为实验组和对照组,各15例。实验组在体外循环预充液中按0.33 m L/kg加入醒脑静注射液,对照组加入等量0.9%氯化钠溶液,分别于体外循环转机前(T1)、主动脉阻断后30 min(T2)、主动脉开放后30 min(T3),主动脉开放后2 h(T4)抽取患者颈静脉血和桡动脉血,检测神经元特异性烯醇化酶(NSE)、同型半胱氨酸(Hcy)浓度及血气指标,并根据血气指标计算上述时间点脑摄氧率(CEO2)。术后采用ICU谵妄评估方法(CAM-ICU)评估术后患者谵妄的发生情况。结果在体外循环心脏手术中,实验组在T2、T3和T4时间点NSE水平低于对照组(P<0.05)。实验组在T3、T4时间点Hcy水平低于对照组(P<0.05)。实验组在T4时间点CEO2高于对照组(P<0.05)。术后患者谵妄发生率实验组为6.7%,对照组为33.3%,差异具有统计学意义(P<0.05)。结论醒脑静注射液在体外循环心脏手术中可以有效调节NSE和Hcy,降低脑部损伤,促进脑功能及早恢复并能有效降低患者术后早期谵妄的发生。展开更多
文摘The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR .This implies that the on line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large scale cultivation of mammalian cells.
文摘摄氧率(OUR)不仅易于在线检测而且可以快速反映细胞的物质和能量代谢状况。为此,利用2 L B.Braun反应器的恒速流加培养工艺,考察了杂交瘤细胞的摄氧率与生长、代谢之间的关系。OUR与活细胞密度成良好的正相关性,但由于氧比消耗速率不是常数,所以二者不呈线性关系,从而利用OUR只能估测,而不能准确计算细胞密度。随着葡萄糖浓度降至低水平,氧比消耗速率、ATP比生成速率下降,而氧与葡萄糖的消耗计量比、细胞对氧的消耗逐增,由氧化磷酸化生成的ATP比例上升,说明葡萄糖代谢途径逐渐从糖酵解途径迁移到三羧酸循环,被氧化程度提高,能量利用率增强。
文摘Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based on pseudo-steady state mass transfer balance, an oxygen transfer model was established.Volumetric oxygen transfer coefficient k_La was estimated as a function of aeration rate and power input per unit volume.Oxygen uptake rate obtained from the macrokinetic model matched well with the oxygen transfer rate estimated by the mass balance model during the whole cultivation.The application of the oxygen transfer model for on-line biomass estimation was also presented.
文摘目的:应用近红外光谱法(NIRS)评估肺表面活性物质(PS)治疗对早产儿脑氧代谢的影响。方法:符合纳入标准的呼吸窘迫综合征(RDS)早产儿,生后2 h内持续正压通气(CPAP)治疗失败的予以插管-给药-拔管法(INSURE)联合PS治疗作为治疗组,单纯CPAP治疗的早产儿作为对照组。治疗开始前、治疗过程及治疗后的各5 min时长窗应用NIRS监测脑氧饱和度(ScO_(2)),同时监测血氧饱和度(SaO_(2)),计算脑摄氧率(FTOE)。结果:2018年3月至2019年2月广州市妇女儿童医疗中心出生的早产儿,符合标准纳入本研究的治疗组24例、对照组25例,两组患儿临床特征及应用PS治疗前的ScO_(2)和FTOE水平比较差异无统计学意义。治疗组给药期间,ScO_(2)水平较其基线水平升高(82.1%±6.4%vs 77.0%±3.7%,t=3.362,P=0.002),FTOE水平较基线水平降低(0.11±0.05 vs 0.19±0.05,t=5.552,P=0.000);给药后5 min ScO_(2)和FTOE均恢复至基线水平。给药期间,治疗组的ScO_(2)变化率(与基线水平的比率)高于对照组[1.049(1.026~1.122)vs 1.000(0.972~1.013),Z=135.0,P=0.001],而治疗组FTOE变化率较对照组大[0.560(0.378~0.794)vs 0.938(0.860~1.110),Z=99.00,P=0.000],相应时间段对照组ScO_(2)和FTOE与基线水平变化率比较差异无统计学意义(P>0.05)。结论:应用PS治疗RDS早产儿时可引起脑氧代谢发生明显变化,ScO_(2)升高,FTOE降低,为临床早产儿呼吸系统疾病的治疗提供更为合理的方式。
