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全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸的含量 被引量:8
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作者 秦菁 魏鲜娥 +2 位作者 蔡伟江 陈彩云 梁洁仪 《食品安全质量检测学报》 CAS 2017年第5期1883-1887,共5页
目的建立全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸。方法样品经上样缓冲液(pH 2.2)溶解提取,按照氨基酸分析仪设定的钠体系程序洗脱,用Na型离子交换色谱柱分离,与茚三酮反应液在135℃下反应,生成可在570 nm和440 nm检测到的蓝色物质... 目的建立全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸。方法样品经上样缓冲液(pH 2.2)溶解提取,按照氨基酸分析仪设定的钠体系程序洗脱,用Na型离子交换色谱柱分离,与茚三酮反应液在135℃下反应,生成可在570 nm和440 nm检测到的蓝色物质,采用外标法定量。结果牛磺酸在4.1065~20.5326μg/mL浓度范围内有良好的线性,r^2=0.999,回收率为101.28%,RSD为2.1%,检出限为15 mg/100 g。结论该方法准确可靠,不需要剧毒衍生试剂,适用于支链氨基酸粉中牛磺酸的测定。 展开更多
关键词 牛磺酸 支链氨基酸粉 保健食品 氨基酸分析仪
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Composition of Starch and Protein in the Endosperm of Newly Generated Double Recessive Waxy and Opaque 2 Maize (Zea mays L.) Genotypes
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作者 N. C. Dang M. Munsch +4 位作者 I. Aulinger W. Renlai H. Le-Huy S. Jampaton P. Stamp 《Journal of Agricultural Science and Technology(B)》 2011年第5期631-637,共7页
Waxy maize with its pure amylopectin starch is the staple food of many ethnic minorities in hilly regions of Southeast Asia (SEA). A combination of waxy and quality protein maize (QPM) traits would improve the qua... Waxy maize with its pure amylopectin starch is the staple food of many ethnic minorities in hilly regions of Southeast Asia (SEA). A combination of waxy and quality protein maize (QPM) traits would improve the quality of protein of waxy maize for human consumption. Double recessive waxy-QPM (wx-o2) genotypes had been generated from Southern Chinese material by haploid induction of crosses heterozygous for the two quality traits with an absolutely conserved waxy type and an improved amino acid profile. The vitreous kernel trait (due to the additional modifier genes present in QPM) was lost in the wx-o2 plant material; this may be due to the waxy mutation, this is anyhow desirable for acceptance as waxy maize is preferred due to its soft grains. The content of the quality limiting amino acid lysine was greatly increased in double recessive wx-o2 genotypes compared to standard waxy maize, but still with a high variation among genotypes for future improvement. Conclusively, it was indeed possible to combine two grain quality mutations successfully within one genotype and prototypes of double quality wx-o2 are available now to contribute to meet human requirements in essential amino acids and thus reduce malnutrition in various regions of Asia. 展开更多
关键词 Quality protein maize GBSS I granule-bound starch synthase I waxy maize
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