Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonary arteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbit...Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonary arteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbits treated with intratracheal bleomycin (BLM). Methods: Immature rabbits were divided into control and BLM group. Two and four weeks after intratracheal normal saline or BLM injection, the systolic, diastolic and mean pulmonary artery pressure (PASP, PADP, MPAP) were measured by micro-catheter; the pathological changes and the expression of VEGFmRNA and eNOSmRNA of endothelial cells in pulmonary arteries were evaluated by HE and in situ hybridization.Results: Two and four weeks after intratracheal injection of BLM, the PASP, PADP and MPAP increased 53%, 49%, 52%in 2 weeks, and 43%, 89%, 56% in 4 weeks; the wall thickness increased and the cavity in middle and small pulmonary arteries became narrow; the Thickness Index (TI) and Area Index (AI) increased 25%, 14% in 2 weeks, and 22%, 24% in 4 weeks; the level of VEGFmRNA and eNOSmRNA expression decreased 46%, 43% in 2 weeks, and 43%, 51% in 4 weeks.There was no significant difference between 2 weeks and 4 weeks BLM groups. Conclusion: The pulmonary artery pressure was elevated, the thickness of wall increased and the cavity became narrow in middle and small pulmonary arteries, and the level of VEGFmRNA and eNOSmRNA expression in pulmonary arterial endothelial cells decreased in immature rabbits after 2 weeks and 4 weeks of intratracheal 4 U/kg BLM injection.展开更多
目的 探讨妊娠期高血压疾病(HDP)胎盘床血流动力学参数与新生儿预后的相关性。方法 回顾性选择符合妊HDP诊断标准HDP患者112例,其中妊娠期高血压疾病组52例、子痫前期组34例及子痫期组26例。另选取正常妊娠妇女50例为对照组。各组根据...目的 探讨妊娠期高血压疾病(HDP)胎盘床血流动力学参数与新生儿预后的相关性。方法 回顾性选择符合妊HDP诊断标准HDP患者112例,其中妊娠期高血压疾病组52例、子痫前期组34例及子痫期组26例。另选取正常妊娠妇女50例为对照组。各组根据观察时间分孕20~25周、孕30~35周。患者临床资料完整,孕30周前诊断为HDP,确诊前均未经药物治疗,孕20~25周、孕30~35周有胎盘血流动力学检查记录。观察患者胎盘床脐动脉血流搏动指数(PI)、阻力指数(RI)及收缩期最大速度/舒张末期速度之比(S/D)。分析各组新生儿出生孕周、检测体质量、观察新生儿窒息(1 min Apgar评分)及羊水粪染程度。探讨HDP患者PI、RI、S/D各自与新生儿出生孕周、体质量、观察新生儿窒息评分、羊水粪染程度的相关性。并进行统计分析。结果 4组孕20~25周和孕30~35周PI、RI及S/D值比较,差异均有统计学意义(t=9.997、5.378、4.393;7.658、6.548、4.321;6.547、5.647、4.542;7.654、4.651、3.214、3.112,均P<0.05)。4组孕20~25周PI、RI及S/D值比较,均为正常组<妊娠高血压疾病组<子痫前期组<子痫期组,差异均有统计学意义(均P<0.05)。4组孕30~35周PI、RI及S/D值比较,均为正常组<妊娠高血压疾病组<子痫前期组<子痫期组,差异均有统计学意义(均P<0.05)。4组新生儿出生孕周、体质量、1 min Apgar评分及羊水粪染程度比较差异均有统计学意义(F=132.581、176.325、131.358、122.364,均P<0.05)。即4组新生儿出生孕周、体质量、1 min Apgar评分比较,均为正常组>妊娠高血压疾病组>子痫前期组>子痫期组,差异均有统计学意义(均P<0.05);4组新生儿羊水粪染程度比较,均为正常组<妊娠高血压疾病组<子痫前期组<子痫期组,差异均有统计学意义(均P<0.05)。HDP患者孕30~35周胎盘床脐动脉血流搏动指数(PI)、阻力指数(RI)及收缩期最大速度/舒张末期速度(S/D)分别与新生儿出生孕周、体质量及1 min Apgar评分均呈负相关性,与羊水粪染程度呈正相关性(均P<0.05)。结论 正常孕妇和HDP患者随着孕周延长胎盘床血流动力学参数PI、RI、S/D均明显降低。HDP者胎盘床血流动力学参数PI、RI、S/D明显大于正常孕妇。HDP患者新生儿出生孕周、体质量、1 min Apgar评分随着病情加重而减少,并且均小于正常组,而羊水粪染程度随着病情加重其评分增加,并且大于正常组。HDP者胎盘床血流动力学参数PI、RI、S/D与新生儿出生孕周、体质量、1 min Apgar评分呈负相关,与羊水粪染程度呈正相关。展开更多
文摘Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonary arteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbits treated with intratracheal bleomycin (BLM). Methods: Immature rabbits were divided into control and BLM group. Two and four weeks after intratracheal normal saline or BLM injection, the systolic, diastolic and mean pulmonary artery pressure (PASP, PADP, MPAP) were measured by micro-catheter; the pathological changes and the expression of VEGFmRNA and eNOSmRNA of endothelial cells in pulmonary arteries were evaluated by HE and in situ hybridization.Results: Two and four weeks after intratracheal injection of BLM, the PASP, PADP and MPAP increased 53%, 49%, 52%in 2 weeks, and 43%, 89%, 56% in 4 weeks; the wall thickness increased and the cavity in middle and small pulmonary arteries became narrow; the Thickness Index (TI) and Area Index (AI) increased 25%, 14% in 2 weeks, and 22%, 24% in 4 weeks; the level of VEGFmRNA and eNOSmRNA expression decreased 46%, 43% in 2 weeks, and 43%, 51% in 4 weeks.There was no significant difference between 2 weeks and 4 weeks BLM groups. Conclusion: The pulmonary artery pressure was elevated, the thickness of wall increased and the cavity became narrow in middle and small pulmonary arteries, and the level of VEGFmRNA and eNOSmRNA expression in pulmonary arterial endothelial cells decreased in immature rabbits after 2 weeks and 4 weeks of intratracheal 4 U/kg BLM injection.
文摘目的 探讨妊娠期高血压疾病(HDP)胎盘床血流动力学参数与新生儿预后的相关性。方法 回顾性选择符合妊HDP诊断标准HDP患者112例,其中妊娠期高血压疾病组52例、子痫前期组34例及子痫期组26例。另选取正常妊娠妇女50例为对照组。各组根据观察时间分孕20~25周、孕30~35周。患者临床资料完整,孕30周前诊断为HDP,确诊前均未经药物治疗,孕20~25周、孕30~35周有胎盘血流动力学检查记录。观察患者胎盘床脐动脉血流搏动指数(PI)、阻力指数(RI)及收缩期最大速度/舒张末期速度之比(S/D)。分析各组新生儿出生孕周、检测体质量、观察新生儿窒息(1 min Apgar评分)及羊水粪染程度。探讨HDP患者PI、RI、S/D各自与新生儿出生孕周、体质量、观察新生儿窒息评分、羊水粪染程度的相关性。并进行统计分析。结果 4组孕20~25周和孕30~35周PI、RI及S/D值比较,差异均有统计学意义(t=9.997、5.378、4.393;7.658、6.548、4.321;6.547、5.647、4.542;7.654、4.651、3.214、3.112,均P<0.05)。4组孕20~25周PI、RI及S/D值比较,均为正常组<妊娠高血压疾病组<子痫前期组<子痫期组,差异均有统计学意义(均P<0.05)。4组孕30~35周PI、RI及S/D值比较,均为正常组<妊娠高血压疾病组<子痫前期组<子痫期组,差异均有统计学意义(均P<0.05)。4组新生儿出生孕周、体质量、1 min Apgar评分及羊水粪染程度比较差异均有统计学意义(F=132.581、176.325、131.358、122.364,均P<0.05)。即4组新生儿出生孕周、体质量、1 min Apgar评分比较,均为正常组>妊娠高血压疾病组>子痫前期组>子痫期组,差异均有统计学意义(均P<0.05);4组新生儿羊水粪染程度比较,均为正常组<妊娠高血压疾病组<子痫前期组<子痫期组,差异均有统计学意义(均P<0.05)。HDP患者孕30~35周胎盘床脐动脉血流搏动指数(PI)、阻力指数(RI)及收缩期最大速度/舒张末期速度(S/D)分别与新生儿出生孕周、体质量及1 min Apgar评分均呈负相关性,与羊水粪染程度呈正相关性(均P<0.05)。结论 正常孕妇和HDP患者随着孕周延长胎盘床血流动力学参数PI、RI、S/D均明显降低。HDP者胎盘床血流动力学参数PI、RI、S/D明显大于正常孕妇。HDP患者新生儿出生孕周、体质量、1 min Apgar评分随着病情加重而减少,并且均小于正常组,而羊水粪染程度随着病情加重其评分增加,并且大于正常组。HDP者胎盘床血流动力学参数PI、RI、S/D与新生儿出生孕周、体质量、1 min Apgar评分呈负相关,与羊水粪染程度呈正相关。
