[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlappe...[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlapped and served as the template to the other DNA strand in amplification. Then, several rounds of over- lap PCR reaction were conducted to mutate the four cysteines in the HP intein into serines, and introduce a linker sequence containing a tag for product detection and purification. [Result] DNA sequencing analysis proved that the four cysteines in the HP intein were successfully mutated into serines; the PCR precursor fragments were also rejoined into an intact HP gene fragment, without introducing any other unex- pected mutation; the DNA linker of 46 bp was successfully inserted into the de- signed HP gene sequences, without any mutation and base pair mismatch. [Conclu- sion] The HP intein was rapidly reconstructed by overlap-extension PCR in this study, which not only expands the application of overlap PCR in protein engineering, but also provides a simple, efficient and highly accurate tool for the reconstruction and modification of intein.展开更多
Low-molecular-weight (LMW) organic acids exist widely in soils and have beenimplicated in many soil processes. The objective of the present paper was to evaluate effect of twoLMW organic acids, citric acid and oxalic ...Low-molecular-weight (LMW) organic acids exist widely in soils and have beenimplicated in many soil processes. The objective of the present paper was to evaluate effect of twoLMW organic acids, citric acid and oxalic acid, on Cl^-adsorption by three variable charge soils, alatosol, a lateritic red soil and a red soil, using a batch method. The results showed that thepresence of citric acid and oxalic acid led to a decrease in Cl^- adsorption with larger decreasesfor citric acid. Among the different soils Cl^- adsorption in the lateritic red soil and the redsoil was more affected by both the LMW organic acids than that in the latosol.展开更多
As the new government of Myanmar has implemented a series of investment-attracting economic reforms since 2011, this paper, using SWOT method, makes an attempt to analyze Yunnan＇s small and medium-sized enterprises ...As the new government of Myanmar has implemented a series of investment-attracting economic reforms since 2011, this paper, using SWOT method, makes an attempt to analyze Yunnan＇s small and medium-sized enterprises （SMEs） investing in Myanmar in order to put forward the countermeasures for its disadvantages and external challenges.展开更多
The holographic dark energy （HDE） is now an studied extensively in the literature. In the derivation of HDE, interesting candidate of dark energy, which has been the black hole entropy plays an important role. In fa...The holographic dark energy （HDE） is now an studied extensively in the literature. In the derivation of HDE, interesting candidate of dark energy, which has been the black hole entropy plays an important role. In fact, the entropy-area relation can be modified due to loop quantum gravity or other reasons. With the modified entropyarea relation, we propose the so-called ＂entropy-corrected holographic dark energy＂ （ECHDE） in the present work. We consider many aspects of ECHDE and tlnd some interesting results. In addition, we briefly consider the so-called ＂entropy-eorreeted agegraphic dark energy＂ （ECADE）.展开更多
AIM:To describe a modified technique for placement of a tracheobronchial self-expanding plastic stent(SEPS) in patients with benign refractory hypopharyngeal strictures in order to improve dysphagia and allow strictur...AIM:To describe a modified technique for placement of a tracheobronchial self-expanding plastic stent(SEPS) in patients with benign refractory hypopharyngeal strictures in order to improve dysphagia and allow stricture remodeling.METHODS:A case series of four consecutive patients with complex hypopharyngeal strictures after combinedtherapy for laryngeal cancer,previously submitted to multiple sessions of dilation without lasting improvement,is presented.All patients underwent placement of a small diameter and unflared tracheobronchial SEPS.Main outcome measurements were improvement of dysphagia and avoiding of repeated dilation.RESULTS:The modified introducer system allowed an easy and technically successful deployment of the tracheobronchial Polyflex stent through the stricture.All four patients developed complications related to stent placement.Two patients had stent migration(one proximal and one distal),two patients developed phanryngocutaneous fistulas and all patients with stents in situ for more than 8 wk had hyperplastic tissue growth at the upper end of the stent.Stricture recurrence was observed at 4 wk follow-up after stent removal in all patients CONCLUSION:Although technically feasible,placement of a tracheobronchial SEPS is associated with a high risk of complications.Small diameter stents must be kept in place for longer than 3 mo to allow adequate time for stricture remodeling.展开更多
Objective To design a technique of esophagus-stomach-abdominal wall drainage for the de-layed intrathoracic esophageal perforation and to improve the therapeutic results. Methods Four patients were treated by this sim...Objective To design a technique of esophagus-stomach-abdominal wall drainage for the de-layed intrathoracic esophageal perforation and to improve the therapeutic results. Methods Four patients were treated by this simplified technique. There were 1 case of lower intrathoracic esophageal perforation to the left thorax , 1 high and 2 middle perforation to the right. This technique used two plastic tubes (chest tube) in a diameter about 1 .2cm . One tube served as an intercostal drainage tube to drain purulent effusion , the other was inserted abdominally through stomach to the esophagus about 10cm above the esophageal perforation. Results The four patients were treated successfully by the esophagus-stomach-abdominal wall drainage. There was no mortality or severe morbidity or complication. Hospitalizations were shortened. Conclusion This technique is simple, safe and effective. It may provide a more promising alternative method of treatment for delayed esophageal perforation, especially in the critically ill patients. The procedure can also be extended to deal with esophagus-stomach anastomotic leak.展开更多
China＇s economic growth has been driven by the dual-engine of domestic demand and external demand. Among which, domestic demand is the main engine originated from China＇s huge domestic market. Due to the advantage o...China＇s economic growth has been driven by the dual-engine of domestic demand and external demand. Among which, domestic demand is the main engine originated from China＇s huge domestic market. Due to the advantage of openness, external demand has a growing contribution to economic growth. For a long term, China will remain in the stage of rapid industrialization, urbanization, marketization and internationalization, therefore China will further expand its domestic consumption and external demand. The undergoing institutional reform will also contribute to the coordinated development of domestic demand and external demand.展开更多
基金Supported by the National High Technology Research and Development Program of China(863Program)(2006AA03Z451)Shanghai Basic Research Key Program(10JC1400300)+1 种基金National Natural Science Foundation of China(31070698)the Fundamental Research Funds for the Central Universities(12D10520)~~
文摘[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlapped and served as the template to the other DNA strand in amplification. Then, several rounds of over- lap PCR reaction were conducted to mutate the four cysteines in the HP intein into serines, and introduce a linker sequence containing a tag for product detection and purification. [Result] DNA sequencing analysis proved that the four cysteines in the HP intein were successfully mutated into serines; the PCR precursor fragments were also rejoined into an intact HP gene fragment, without introducing any other unex- pected mutation; the DNA linker of 46 bp was successfully inserted into the de- signed HP gene sequences, without any mutation and base pair mismatch. [Conclu- sion] The HP intein was rapidly reconstructed by overlap-extension PCR in this study, which not only expands the application of overlap PCR in protein engineering, but also provides a simple, efficient and highly accurate tool for the reconstruction and modification of intein.
基金Project supported by the Knowledge Innovation Program Foundation of the Chinese Academy of Sciences (No. ISSASIP0108) the National Natural Science Foundation of China (No. 40271062).
文摘Low-molecular-weight (LMW) organic acids exist widely in soils and have beenimplicated in many soil processes. The objective of the present paper was to evaluate effect of twoLMW organic acids, citric acid and oxalic acid, on Cl^-adsorption by three variable charge soils, alatosol, a lateritic red soil and a red soil, using a batch method. The results showed that thepresence of citric acid and oxalic acid led to a decrease in Cl^- adsorption with larger decreasesfor citric acid. Among the different soils Cl^- adsorption in the lateritic red soil and the redsoil was more affected by both the LMW organic acids than that in the latosol.
文摘As the new government of Myanmar has implemented a series of investment-attracting economic reforms since 2011, this paper, using SWOT method, makes an attempt to analyze Yunnan＇s small and medium-sized enterprises （SMEs） investing in Myanmar in order to put forward the countermeasures for its disadvantages and external challenges.
基金Supported by the Excellent Young Scholars Research Fund of Beijing Institute of Technology
文摘The holographic dark energy （HDE） is now an studied extensively in the literature. In the derivation of HDE, interesting candidate of dark energy, which has been the black hole entropy plays an important role. In fact, the entropy-area relation can be modified due to loop quantum gravity or other reasons. With the modified entropyarea relation, we propose the so-called ＂entropy-corrected holographic dark energy＂ （ECHDE） in the present work. We consider many aspects of ECHDE and tlnd some interesting results. In addition, we briefly consider the so-called ＂entropy-eorreeted agegraphic dark energy＂ （ECADE）.
文摘AIM:To describe a modified technique for placement of a tracheobronchial self-expanding plastic stent(SEPS) in patients with benign refractory hypopharyngeal strictures in order to improve dysphagia and allow stricture remodeling.METHODS:A case series of four consecutive patients with complex hypopharyngeal strictures after combinedtherapy for laryngeal cancer,previously submitted to multiple sessions of dilation without lasting improvement,is presented.All patients underwent placement of a small diameter and unflared tracheobronchial SEPS.Main outcome measurements were improvement of dysphagia and avoiding of repeated dilation.RESULTS:The modified introducer system allowed an easy and technically successful deployment of the tracheobronchial Polyflex stent through the stricture.All four patients developed complications related to stent placement.Two patients had stent migration(one proximal and one distal),two patients developed phanryngocutaneous fistulas and all patients with stents in situ for more than 8 wk had hyperplastic tissue growth at the upper end of the stent.Stricture recurrence was observed at 4 wk follow-up after stent removal in all patients CONCLUSION:Although technically feasible,placement of a tracheobronchial SEPS is associated with a high risk of complications.Small diameter stents must be kept in place for longer than 3 mo to allow adequate time for stricture remodeling.
文摘Objective To design a technique of esophagus-stomach-abdominal wall drainage for the de-layed intrathoracic esophageal perforation and to improve the therapeutic results. Methods Four patients were treated by this simplified technique. There were 1 case of lower intrathoracic esophageal perforation to the left thorax , 1 high and 2 middle perforation to the right. This technique used two plastic tubes (chest tube) in a diameter about 1 .2cm . One tube served as an intercostal drainage tube to drain purulent effusion , the other was inserted abdominally through stomach to the esophagus about 10cm above the esophageal perforation. Results The four patients were treated successfully by the esophagus-stomach-abdominal wall drainage. There was no mortality or severe morbidity or complication. Hospitalizations were shortened. Conclusion This technique is simple, safe and effective. It may provide a more promising alternative method of treatment for delayed esophageal perforation, especially in the critically ill patients. The procedure can also be extended to deal with esophagus-stomach anastomotic leak.
文摘China＇s economic growth has been driven by the dual-engine of domestic demand and external demand. Among which, domestic demand is the main engine originated from China＇s huge domestic market. Due to the advantage of openness, external demand has a growing contribution to economic growth. For a long term, China will remain in the stage of rapid industrialization, urbanization, marketization and internationalization, therefore China will further expand its domestic consumption and external demand. The undergoing institutional reform will also contribute to the coordinated development of domestic demand and external demand.
